Dragonflies Feeding on Amphipods; Size Does Matter Linda White, Kenny Danner, Shane Murnan, Craig Henson, and Lori Espieg ...
Introducing…our study subject  <ul><li>We will be studying the  Hylella  amphipod. </li></ul>
Introducing…our prey <ul><li>Dragonfly nymph </li></ul>
  Step 1   <ul><li>We added 400 mL of non-chlorinated well water to fifteen individual sandwich sized 900 mL “quart” servi...
Step 2 <ul><li>We placed four four inch segments of ambulia plastic aquarium plants.  </li></ul>
Step 3 <ul><li>All of the containers were left overnight to reach ambient temperature and oxygenate the water.  </li></ul>
Step 4 <ul><li>Dragonfly larvae were separated into individual plastic beverage containers overnight to rest.  Dragonfly l...
Step 5 <ul><li>We separated all of the amphipods into four categories; small, medium, large, and females with broods. The ...
Step 6 <ul><li>We placed eight small, eight medium, and eight large amphipods into each container. </li></ul>
Step 7   <ul><li>After allowing the amphipods to rest in the containers, we placed one dragonfly larvae in each container....
Step 8 <ul><li>After allowing nature to take its course, we returned to examine our results. </li></ul>
 
Step 9 <ul><li>The dragonfly larvae had a bigger appetite than expected.  We found no amphipods.  All of our test subjects...
Step 10 <ul><li>We conducted our experiment a second time using eight of each size amphipod in each arena.  We placed the ...
Step 11 <ul><li>Our dragonfly larvae were left in the arenas for twelve hours.  After twelve hours the dragonfly larvae we...
Step 12 <ul><li>We placed the remaining amphipods and dragonfly larvae in a vial with ethanol solution “killing juice” to ...
Step 13 <ul><li>We sorted the amphipods based on size remaining on Petri dishes for each arena. </li></ul>
Step 14 <ul><li>Using a microscope, we measured the head length of each of the remaining amphipods. </li></ul><ul><li>We m...
Step 15 <ul><li>All of our data was entered into a spreadsheet so we could draw conclusions based on our data. </li></ul>
Results <ul><li>We determined that the dragonfly larvae preferred the medium size amphipods to the small or large amphipod...
 
Conclusions <ul><li>Through the analysis of our data, we determined that the dragonfly larvae prefer amphipods that are me...
Ideas for Future Study <ul><li>Comparing females with and without broods to males of the same size. </li></ul><ul><li>Comp...
More Ideas for Future Study <ul><li>Painting black dots on males to determine if it increases visibility or attractiveness...
Potential Predators <ul><li>Toe Biters </li></ul><ul><li>Crawfish </li></ul><ul><li>Tadpoles </li></ul><ul><li>Fish </li><...
Disclaimer
<ul><li>Questions? </li></ul>
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Final Presentation: PowerPoint File

  1. 1. Dragonflies Feeding on Amphipods; Size Does Matter Linda White, Kenny Danner, Shane Murnan, Craig Henson, and Lori Espieg Research Scientist: John White
  2. 2. Introducing…our study subject <ul><li>We will be studying the Hylella amphipod. </li></ul>
  3. 3. Introducing…our prey <ul><li>Dragonfly nymph </li></ul>
  4. 4. Step 1 <ul><li>We added 400 mL of non-chlorinated well water to fifteen individual sandwich sized 900 mL “quart” serving containers . </li></ul>
  5. 5. Step 2 <ul><li>We placed four four inch segments of ambulia plastic aquarium plants. </li></ul>
  6. 6. Step 3 <ul><li>All of the containers were left overnight to reach ambient temperature and oxygenate the water. </li></ul>
  7. 7. Step 4 <ul><li>Dragonfly larvae were separated into individual plastic beverage containers overnight to rest. Dragonfly larvae were not fed to ensure that each dragonfly had an appetite. </li></ul>
  8. 8. Step 5 <ul><li>We separated all of the amphipods into four categories; small, medium, large, and females with broods. The females with broods were placed in a separate container. </li></ul>
  9. 9. Step 6 <ul><li>We placed eight small, eight medium, and eight large amphipods into each container. </li></ul>
  10. 10. Step 7 <ul><li>After allowing the amphipods to rest in the containers, we placed one dragonfly larvae in each container. </li></ul>
  11. 11. Step 8 <ul><li>After allowing nature to take its course, we returned to examine our results. </li></ul>
  12. 13. Step 9 <ul><li>The dragonfly larvae had a bigger appetite than expected. We found no amphipods. All of our test subjects had been consumed. </li></ul>
  13. 14. Step 10 <ul><li>We conducted our experiment a second time using eight of each size amphipod in each arena. We placed the females with broods in a separate container. </li></ul>
  14. 15. Step 11 <ul><li>Our dragonfly larvae were left in the arenas for twelve hours. After twelve hours the dragonfly larvae were separated from the amphipods. </li></ul>
  15. 16. Step 12 <ul><li>We placed the remaining amphipods and dragonfly larvae in a vial with ethanol solution “killing juice” to preserve them for measuring. </li></ul>
  16. 17. Step 13 <ul><li>We sorted the amphipods based on size remaining on Petri dishes for each arena. </li></ul>
  17. 18. Step 14 <ul><li>Using a microscope, we measured the head length of each of the remaining amphipods. </li></ul><ul><li>We measured the head width of each dragonfly larvae. </li></ul>
  18. 19. Step 15 <ul><li>All of our data was entered into a spreadsheet so we could draw conclusions based on our data. </li></ul>
  19. 20. Results <ul><li>We determined that the dragonfly larvae preferred the medium size amphipods to the small or large amphipods. </li></ul>
  20. 22. Conclusions <ul><li>Through the analysis of our data, we determined that the dragonfly larvae prefer amphipods that are medium sized. </li></ul><ul><li>Reasons for this could be the energy used to catch the amphipod in relation to the energy gained by consuming the amphipod. </li></ul>
  21. 23. Ideas for Future Study <ul><li>Comparing females with and without broods to males of the same size. </li></ul><ul><li>Comparing females with and without broods. </li></ul><ul><li>Increase the variation of dragonfly larvae size. </li></ul>
  22. 24. More Ideas for Future Study <ul><li>Painting black dots on males to determine if it increases visibility or attractiveness. </li></ul><ul><li>Analyze energy content to determine if females with broods provide a higher energy return for dragonfly larvae. </li></ul><ul><li>Introduce other predators to determine which size amphipod they prefer. </li></ul>
  23. 25. Potential Predators <ul><li>Toe Biters </li></ul><ul><li>Crawfish </li></ul><ul><li>Tadpoles </li></ul><ul><li>Fish </li></ul>
  24. 26. Disclaimer
  25. 27. <ul><li>Questions? </li></ul>

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