comparison of light and electron microscopes

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  • 1. Comparing Light and Electron MicroscopesAdvantages of Electron Disadvantages of Light Microscopes Microscope• Greater magnification up • Magnification only up to to 1,000,000 x 1000-1,500 x• Greater depth of field • Less depth of field (With SEMs, 3-D ) • May involve complex• No complex lighting lighting requirements• Greater resolution -1 nm • Less resolution - 1 micron
  • 2. Light Microscopes
  • 3. Light microscope images QuickTime™ and a QuickTime™ and aTIFF (Uncompressed) decompressor TIFF (Uncompressed) decompressor are needed to see this picture. are needed to see this picture. QuickTime™ and a QuickTime™ and a TIFF (Uncompressed) decompressor TIFF (Uncompressed) decompressor are needed to see this picture. are needed to see this picture.
  • 4. Microscopes ContinuedDisadvantages of Advantages of Light Electron Microscope Microscope• Expensive (1 million) • Cheaper (100)• Expensive to Run • Cheaper to Run• Image Black and • Color image White • Live or Dead• Dead Specimen Specimen• Requires Vacuum • No Vacuum Required• Expert Preparation • Simple Preparation• Special Large Room • Small and Portable• Affected by Magnetic • Unaffected by Fields Magnetic Fields
  • 5. Electron Microscope images QuickTime™ and a TIFF (Uncompressed) decompressor QuickTime™ and a are needed to see this picture. TIFF (Uncompressed) decompressor are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture.
  • 6. SEMs TEMs QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. QuickTime™ and aTIFF (Uncompressed) decompressor are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture.
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  • 9. QuickTime™ and aTIFF (Uncompressed) decompressor are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. 40 x (SEM) 435x (SEM) QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture.
  • 10. Eye of a Mosquito QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture.
  • 11. QuickTime™ and aTIFF (Uncompressed) decompressor are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. 1760 x