Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative  Bacteria Escherichia coli
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Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli

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Since plants are used as therapeutic agents, the study was conducted to
evaluate the second metabolites of Momordica charantia Linn’s extract and it’s
antibacterial activities.

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Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative  Bacteria Escherichia coli Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli Presentation Transcript

  • Proponents John Rovic Ngo,Trisha Reyes,Lester Almarez August2013 Momordica charantia L. Extract as a Prophylactic Medicine for Gram- Negative Bacteria Escherichia coli A Science Research Project Presented to The Faculty of Science and Technology
  • Abstract • Since plants are used as therapeutic agents, the study was conducted to evaluate the second metabolites of Momordica charantia Linn’s extract and it’s antibacterial activities. Studies on the plant’s extract itself, 75% ethyl alcohol and sample free-disk as the control setups using Disc Agar Diffusion Method.
  • • The microorganism.used is Gram Negative bacteria Escherichia coli. The maximum activity was observed against the said bacteria. The minimum zone of inhibitory concentration ranged at 10mm on microorganism. As conducted, Momordica charantia L. was observed to have antibacterial activity and can be used as medicinal purposes.
  • Introduction • Bacteria are living things that are ubiquitous. Most of them won’t hurt you. Many are helpful for digestion, destroy disease-causing cells and give the body needed vitamins. But infectious bacteria can cause severe illness. They produce themselves quickly inside the body. Many emit chemicals such as toxins, which can damage tissues.One of the common bacteria that infect us is the gram-negative Escherichia coli. • So, we, the proponents tried to find a resourceful way of medication for Antimicrobial diseases using Momordica charantia Linn extract.
  • Some Terms Used • Momordica Charantia • Escherichia coli • Evaporation • Inoculation
  • Methodology • Sterilization Of Materials • Extraction of Plant Material • Test Organism • Control Set-Up(s) • Inoculum Preparation • Inoculation • Application of Paper Discs With Extracts • Gathering Of Results
  • Gathering of Materials
  • Sterilization of Materials
  • Extraction Of Plant Material
  • Test Organisms
  • Research Paradigm
  • Results
  • Legends Disc: 10 mm diameter Reactivity Rating: 0 – None (No detectable zone around or under specimen) 1 – Slight (Some malformed or degenerated cells under the specimen) 2 – Mild (Zone limited under the specimen) 3 – Moderate (Zone extends 5 to 10 mm beyond specimen) 4 – Severe (Zone extends greater than 10 mm beyond specimen) Inhibitory Activity Rating: (+++) complete, (++) partial, (+) slight and (-) negative
  • Results and Interpretation • The sample, “Ampalaya Extract”, produced complete inhibitory activity (+++) with mild reactivity (2) against test organism, Escherichia coli; The sample “Alcohol”, which served as control, had negative inhibitory activity (-) and no reactivity (0) against the test organism, E. coli. The sample free disc, which served as negative control, produced negative inhibitory activity (-) and no reactivity (0) against the test organism.
  • Discussion • In this experiment, 3 set-ups were used to determine on which will be the most effective prophylactic medicine for gram –ve bacteria Escherichia coli diseases. • From the tabulation, the “Ampalaya Extract” produced the positive result of reactivity.This shows the capabilities of Momordica charantia L.’s extract can be a new medicine for gram –ve bacteria Escherichia coli.
  • Conclusion • The proponents’ research proved that the application of Momordica charantia L.As significance to be the prophylactic medicine for E. coli. • This concludes to the fact that the pure extract of the said plant is a good Medicine for diseases of the said bacteria. • Therefore, the use of the extract of ampalaya can be an alternative agent for E.coli.
  • Bibliography • Seenivasan Prabuseenivasan, Manickkam Jayakumar, Savarimuthu Ignacimuthu: In vitro antibacterial activity of some plant essential oils. BMC Complementary and Alternative Medicine., 2006, 6:39 • Mukesh Chandra Sharma, Smita Sharma: Preliminary Phytochemical and Antimicrobial Investigations of the Aqueous Extract of Ixora coccinea Linn and Commelina benghalensis L. on Gram-Positive and Gram-Positive Microorganisms. Middle-East Journal of Scientific Research., 6(5): 436-439, 2010 • V. Ravi Kumar, K. Ravi Shankar, P. Priyabhandhavi: In vitro evaluation of Antioxidant activity of Ethanolic leaf extract of Ixora coccinea. International Journal of Pharmaceutical, Chemical and Biologica Sciences., 3(2): 380- 287 • P.G. Latha, T.K. Abraham, K.R. Panikkar: Antimicrobial Properties of Ixora coccinea L. (Rubiaceae). Ancient Science of Life., Vol. 14: 286-291; April 4, 1995 • Nagaraj Selvaraj, Baskar Lakshmanan, Papiya Mitra Mazumder, Muthukumar Karuppasamy, Simon Santosh Jena, Ashok Kumar Pattnaik: Evaluation of wound healing and antimicrobial potentials of Ixora coccinea root extract. Asian Pacific Journal of Tropical Medicine., 2011, 959-963 • Sowjanya Pulipati, Sushma P. V, Jhansi Lakshmi, P. Srinivasa Babu: A Comparative Antibacterial Study of Ixora coccinea L. Plants with Red, Orange, Pink and White Flowers. AJPRHC., Vol. 4: Issue 1: 7-10 • S. Nejad Ebrahimi, J. Hadian, M.H. Mirjalili, A. Sonboli, M. Yousefzadi: Essential oil composition and antibacterial activity of Thymus caramanicus at different phonological stages. Food Chemistry., 2008, 927-931 • Imael Henri Nesto Bassole, H. Rodolfo Juliani: Essential Oils in Combination and their Antimicrobial Properties. Molecules 2012., 17, 3989-4006 • J.L. Rios, M.C. Recio: Medicinal Plants and Antimicrobial Activity. Journal of Ethnopharmacology 100., 2005, 80- 85 • K.A. Hammer, C.F. Carson, T.V. Riley: Antimicrobial Activity of Essential Oils and Other Plant Extracts. Journey of Applied Microbiology., 1990, 86 985-990