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Extracting DNA from strawberries
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Extracting DNA from strawberries

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Extracting DNA from strawberries

Extracting DNA from strawberries

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Extracting DNA from strawberries Extracting DNA from strawberries Presentation Transcript

  • Extracting DNA from Strawberries
    Tania Ramos
    Rossini Batino
    Vivian Pham
    Anthony Mendoza
  • PURPOSE
    To see if we can extract DNA from strawberries at different temperature levels.
  • HYPOTHESIS
    We predicted that at lower alcohol temperature the DNA from the strawberries will be extracted most efficiently.
    View slide
  • Materials
    Ziplocs
    coffee filters
    Toothpicks
    Camera
    Laptop
    View slide
  • Procedures
    Step 1.3 beakers with 3 diff. alcohol temp.
    Put strawberries in the bag Crush strawberries
    Step 2 mix salt, tap water and soap . And add it to the crushed strawberries
    Step 3 filter with coffee filter
    Step 4 Take the filter off and add the ice cold alcohol to the mixture in the glass.
    Step 5 After about 5 minutes, you should see a clumpy white substance beginning to form. This is the DNA. The alcohol allows the DNA to float. Use the toothpick or the craft stick to pick up the DNA so you can look at its strands more closely.
  • Procedure
    Step 1
    +
    Step 2
    Cold Regular Hot
    Step 3
  • REGULAR
    HOT
    COLD
  • Data
  • Conclusions
    We concluded that our hypothesis was incorrect: DNA CANbe extracted from all alcohol temperatures.
  • Work Cited
    Cziurczack, Ellen. "How to Extract DNA from strawberries". ehow. June 3,2010 <http://www.ehow.com/how_5031799_extract-dna-strawberry.html>.
    "Alcohol boiling points". ToolBox. June 3,2010 <http://www.engineeringtoolbox.com/boiling-points-fluids-gases-d_155.html>.
    "Dangers to boiling rubbing alcohol". The Straight Dope. June 1, 2010 <http://boards.straightdope.com/sdmb/showthread.php?t=284931>.
    This project is from the "Understanding Genetics: Human Health and the Genome" exhibit at The Tech Museum of Innovation in San Jose, CA: The Tech, 2004. "Making Medicine: Do-It-Yourself Strawberry DNA," The Tech Museum of Innovation, San Jose, CA. [accessed March 6, 2007]http://www.thetech.org/genetics/medicine.php
    Another similar project can be found from the Santa Clara College Biotechnology Education Program (SCCBEP) at San Jose State University: SCCBEP, 2002. "DNA Spooling from Strawberries," Santa Clara College Biotechnology Education Program (SCCBEP), San Jose State University, CA. [accessed March 6, 2007] http://www.science.sjsu.edu/sccbep/resources/curriculum/dna_spooling_strawberry.pdf
    Visit the online "Understanding Genetics: Human Health and the Genome" exhibit at the Tech Museum of Innovation and click on the "Zooming Into DNA" online exhibit. This is a great way to understand and visualize where the DNA in your body is: Rosa, C. et. al., 2007. "Zooming Into DNA," The Tech Museum of Innovation, San Jose, CA. [accessed March 6, 2007]http://www.thetech.org/genetics/index.php
    Want to try a DNA Extraction the way it is done in a real laboratory? Try the DNA Extraction Virtual Laboratory from the University of Utah: Univ. of Utah, 2006. "DNA Extraction Virtual Lab," The Genetic Science Learning Center, University of Utah, Salt Lake City, UT. [accessed March 6, 2007] http://learn.genetics.utah.edu/units/biotech/extraction/