Lab'InSight Toxicological Risk Assessment présentation ULB 24.10.13

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Laboratory of Cancerology
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Lab'InSight Toxicological Risk Assessment présentation ULB 24.10.13

  1. 1. Toxicological Risk Assessment An initiative of 24 October 2013 - UCL - Brussels (Woluwe) 1
  2. 2. Laboratory of Cancerology and Experimental Toxicology - Faculty of Pharmacy Université Libre de Bruxelles Pr Véronique Mathieu MD, Ph.D. Associate Professor 2
  3. 3. Fields of expertise and industrial targets Fields of expertise • • • • Natural (derived) compounds Apoptosis-resistance migration- invasion Anti-cancer activities • • • • Galectin-1 Actin Gene silencing: siRNA Videomiscroscopy Industrial applications fields • Discovery of new chemical entities to combat apoptotic resistant cancers like glioma, melanoma, non-small cell lung carcinoma…. 3
  4. 4. Applied research projects: example of galectin-1 Pub. No.: WO/2006/108474 Publication Date: 19.10.2006 Chapter 2 Demand Filed: 09.02.2007 International Application No.: PCT/EP2006/00217 0 International Filing Date: 09.03.2006 IPC: C12N 15/11 (2006.01), A61K 48/00 (2006.01) Applicants: UNIVERSITE LIBRE DE BRUXELLES [BE/BE]; Avenue F.D. Roosevelt 50, CP 161, B-1050 Brussels (BE) (All Excep UNIVERSITE CATHOLIQUE DE LOUVAIN [BE/BE]; Place De L'université 1, B-1348 Louvain-la-Neuve (BE) (All Exce 4 CAMBY, Isabelle [BE/BE]; Rue Ferrer 22, B-1480 Tubize (BE) (US Only). HENRIET, Patrick [BE/BE]; Rue De La Briqueterie 17, B-1400 Nivelles (BE) (US Only).
  5. 5. Special equipments Examples • • • • • • • Culture rooms Flow cytometry (3 colors) Fluorescent microscope ELISA (RT)-PCR, qPCR WB … 16 videomicoscopic devices Quantitative videomicroscopy > 20 years of expertise • • Animal facilities and cancer aggressive models 5
  6. 6. Computer-Assisted Phase-Contrast Microscopy (Quantitative Videomicroscopy) SKMEL28 Melanoma Cells / Control Debeir O et al. IEEE Trans Med Imaging 2005 6
  7. 7. The cells are cultured for 3 days in presence of the compound x 250 Dumont P et al., Neoplasia 2007 Anti-adhesive ? Computer assisted videomicroscopy Increasing differentiation Soft agar test Cytostatic ? MTT colorimetric test Increasing proliferation potentiel Cytotoxic ?
  8. 8. Services provided to companies Examples • MTT assay on normal and cancer cells • Quantitative videomicroscopy experiment • Cell cycle and / or apoptosis analysis • ROS production analysis • Migration assays (boyden chambers, quantitative scratch wound assay) • Actin or tubulin cytoskeleton analysis (fluorescence) • Mechanism of action deciphering (adapted to your project) • Maximal tolerated dose in vivo in conventional mice • Anti-cancer activity on cancer bearing mice • … 8
  9. 9. Training proposals to companies • PhD training • Post-Doctoral fellowship 9
  10. 10. Contacts Université Libre de Bruxelles - Faculty of Pharmacy Laboratory of Cancerology and Experimental Toxicology Boulevard du Triomphe Campus de la Plaine, CP205/1 Building BC 1050 Brussels http://www.ulb.ac.be/rech/inventaire/unites/ULB646.html Pr Véronique Mathieu Associate Professor vemathie@ulb.ac.be  +32 478 317 388 Pr Jean-Michel Kauffmann Head of the Laboratory jmkauf@ulb.ac.be  +32 2 650 52 15 10

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