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ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic
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ICSI Equipment, Practitioner & Technique: Do these factors affect embryo development? By Dr Cristina Hickman, Head of Embryology at Boston Place Clinic

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  • 1. ICSI Equipment, Practitioner & Technique: cristina.hickman@bostonplaceclinic.co.uk 02079930870 Dr Cristina Hickman Head of Embryology at Boston Place Clinic Do these factors affect embryo development?
  • 2. Disclosure: No commercial or financial relationship with cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 3. cristina.hickman@bostonplaceclinic.co.uk 02079930870 ICSI  Embryo Development
  • 4. ICSI: Equipment cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 5. ICSI: Equipment cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 6. ICSI: Equipment cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 7. Practitioner A Practitioner B cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 8. Practitioner vs Technique - Method cristina.hickman@bostonplaceclinic.co.uk 02079930870 Day 0 embryos blindly selected for transfer Performance Indicators Categorical data assessed using Chi-squared test Morphokinetics Continuous data analysed using a generalised linear model (patient as random factor)
  • 9. cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 10. cristina.hickman@bostonplaceclinic.co.uk 02079930870 Total number of oocytes injected 82 78 Practitioner A/Practitioner B 44/38 42/36 NS
  • 11. Results cristina.hickman@bostonplaceclinic.co.uk 02079930870 0 50 100 150 t2 t3 t4 t5 t6 t7 t8 t9 tsB tB teB tHB Hourspostinsemination
  • 12. Results cristina.hickman@bostonplaceclinic.co.uk 02079930870 0 5 10 15 CC2 S2 I3 IntegraTI P=0.004 P=0.06.
  • 13. Results cristina.hickman@bostonplaceclinic.co.uk 02079930870 0 50 100 Fertilisation Degeneration 3PN appearance Even first cell division Selected for transfer Day 5 utilisation I3 IntegraTI * power of 0.8 for detecting 12% difference in fertilisation rate
  • 14. EXPERIMENT 2: ICSI Technique cristina.hickman@bostonplaceclinic.co.uk 02079930870 • Which ICSI events need to be controlled to maximise embryo viability? • Two embryologists independently assessed the videos of 108 individual ICSIs from 17 patients • Average age = 39.4 years cycle number =2.8
  • 15. cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 16. cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 17. cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 18. cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 19. cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 20. cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 21. cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 22. cristina.hickman@bostonplaceclinic.co.uk 02079930870
  • 23. Conclusion cristina.hickman@bostonplaceclinic.co.uk 02079930870 1. This preliminary assessment suggests that the ICSI injection events observed do not affect fertilisation or embryo morphokinetics 2. Equipment / Practitioner did not affect time to reach cell stages from two cells to hatching blastocyst 3. ICSI equipment can have an effect on i. EMBRYO DEVELOPMENT: I3 embryos two times more likely to be selected for transfer than IntegraTI i. MORPHOKINETICS: I3 longer CC2 than IntegraTI 4. This study not only validates the I3 as performing at least equal to the established IntegraTI system, but also demonstrates that ICSI equipment can have an effect on embryo morphokinetic development
  • 24. Thank you

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