Your SlideShare is downloading. ×
Faculty of Bioengineering Institute of Condensed Matter and Nanosciences Center for Research and Engineering on Biomateria...
Life expectancy increases continuously                     100 survival rate (%)                     80                   ...
More and more spare parts are needed
Shortage of donors andsecondary effects restrictthe use of transplantation
Artificial deviceshave a limited lifetime  and do not adaptto the host organism
Tissue engineering relies on the use of living cells to replace, repair or regenerate damaged tissues
Cells can be seededin a biodegradable scaffold
Self-standing cell sheets are an alternative to scaffolds
Cell growth isanchorage-dependent
Trypsin-induced cell release           disrupts cell-cell junctionstrypsin   and the extracellular matrix
Can we keep cell sheets intact ??
The properties of smart systemschange when applying a stimulus
PNIPAM is a thermoresponsive polymer   showing a transition temperature   close to physiological conditions               ...
PNIPAM can be immobilised at interfaces                ~30°C
Intact cell sheets                  grown on PNIPAM                  can be harvested              by decreasing temperatu...
Cell sheets can potentially be used       for tissue engineering                                      Yang et al., 2005
Cornea can be regrown from    oral mucosal cells seeded           on PNIPAMSutureless transplantation is possibleowing to ...
100survival rate (%)                    80                    60                                                          ...
ResearchTalks Vol. 2 - From the design of smart surfaces to tissue engineeri…
ResearchTalks Vol. 2 - From the design of smart surfaces to tissue engineeri…
Upcoming SlideShare
Loading in...5
×

ResearchTalks Vol. 2 - From the design of smart surfaces to tissue engineeri…

2,430

Published on

Tissue engineering is a promising approach to repair damaged or diseased tissues.

Published in: Health & Medicine, Technology
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
2,430
On Slideshare
0
From Embeds
0
Number of Embeds
3
Actions
Shares
0
Downloads
0
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Transcript of "ResearchTalks Vol. 2 - From the design of smart surfaces to tissue engineeri…"

  1. 1. Faculty of Bioengineering Institute of Condensed Matter and Nanosciences Center for Research and Engineering on Biomaterials UCLouvainFrom the design of smart surfaces to tissue engineering Christine Dupont-Gillain
  2. 2. Life expectancy increases continuously 100 survival rate (%) 80 1940 2006 60 1901 40 20 0 0 10 20 30 40 50 60 70 80 90 100 age (years)data for USA, http://www.cdc.gov
  3. 3. More and more spare parts are needed
  4. 4. Shortage of donors andsecondary effects restrictthe use of transplantation
  5. 5. Artificial deviceshave a limited lifetime and do not adaptto the host organism
  6. 6. Tissue engineering relies on the use of living cells to replace, repair or regenerate damaged tissues
  7. 7. Cells can be seededin a biodegradable scaffold
  8. 8. Self-standing cell sheets are an alternative to scaffolds
  9. 9. Cell growth isanchorage-dependent
  10. 10. Trypsin-induced cell release disrupts cell-cell junctionstrypsin and the extracellular matrix
  11. 11. Can we keep cell sheets intact ??
  12. 12. The properties of smart systemschange when applying a stimulus
  13. 13. PNIPAM is a thermoresponsive polymer showing a transition temperature close to physiological conditions ~30°C
  14. 14. PNIPAM can be immobilised at interfaces ~30°C
  15. 15. Intact cell sheets grown on PNIPAM can be harvested by decreasing temperature37°C 20°C
  16. 16. Cell sheets can potentially be used for tissue engineering Yang et al., 2005
  17. 17. Cornea can be regrown from oral mucosal cells seeded on PNIPAMSutureless transplantation is possibleowing to intact extracellular matrixacting as a glue Yang et al., 2007
  18. 18. 100survival rate (%) 80 60 We need spare parts 40 20 0 0 10 20 30 40 50 60 70 80 90 100 age (years) Tissue engineering relies on the use of cells to produce functional tissues Smart surfaces can be designed to allow intact cell sheets to be harvested, opening perspectives in tissue engineering

×