Topic 4: Chapter 5
EnzymesEnzymes –– Part 1Part 1
Biological
Biological
Catalysts
Catalysts
What are Catalysts?
• Substances which can alter or
speed up a chemical reaction,
• without itself being chemically
change...
CatalystsCatalysts
(A) Inorganic
• Iron – Haber process
• Vanadium (v) Oxide –
production of sulphuric
acid
• Manganese (I...
• Enzymes are biological catalysts
• which are mainly proteins that are produced
by living cells.
• Enzymes can be DENATUR...
The function of enzymes
• How is energy released from sugar (glucose)?
• But how can our cells provide such high
temperatu...
The function of enzymes
Lower Activation EnergyLower Activation Energy
• Activation Energy: Energy required to start a
che...
Enzyme-Catalyzed ReactionsEnzyme-Catalyzed Reactions
(A) Synthesis of
complex substances
Anabolic Reactions
• Protein synt...
Enzyme-Catalyzed Anabolic ReactionsEnzyme-Catalyzed Anabolic Reactions
Protein SynthesisProtein Synthesis
• Synthesis of p...
Enzyme-Catalyzed Catabolic ReactionsEnzyme-Catalyzed Catabolic Reactions
DigestionDigestion
• Breakdown of complex food
su...
Enzyme-Catalyzed Catabolic ReactionsEnzyme-Catalyzed Catabolic Reactions
Cellular RespirationCellular Respiration
• Oxidat...
Enzyme-Catalyzed Catabolic ReactionsEnzyme-Catalyzed Catabolic Reactions
Detoxification in the liver and bloodDetoxificati...
Classification of enzymes
HydrolasesHydrolases
• Enzymes that catalyzes hydrolytic
reactions which involves the
addition o...
Classification of enzymes
HydrolasesHydrolases
Types of
Hydrolases
Example(s) Substrate
Carbohydrases Salivary and pancrea...
Classification of enzymes
Oxidation-Reduction EnzymesOxidation-Reduction Enzymes
• Enzymes that
are involved in
cellular
r...
15
Test yourself
With questions in
Textbook Page 75
16
Test yourself – pg 75 - answers
1
An enzyme is a biological
catalyst made of protein. It
alters the rate of a chemical
...
17
Test yourself – pg 75 - answers
2
Yes, the statement is valid. Large,
insoluble molecules such as
carbohydrates, fats a...
18
Test yourself – pg 75 - answers
3
Meat contains mostly proteins,
hence we can infer that this
enzyme acts on proteins. ...
19
Test yourself – pg 75 - answers
4
A very rapid, violent chemical
reaction would take place, with
a thick layer of foam ...
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Topic 4 chapter 5 part 1 enzymes - biological catalysts

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Topic 4 chapter 5 part 1 enzymes - biological catalysts

  1. 1. Topic 4: Chapter 5 EnzymesEnzymes –– Part 1Part 1 Biological Biological Catalysts Catalysts
  2. 2. What are Catalysts? • Substances which can alter or speed up a chemical reaction, • without itself being chemically changed at the end of the reaction. • Substances which can alter or speed up a chemical reaction, • without itself being chemically changed at the end of the reaction.
  3. 3. CatalystsCatalysts (A) Inorganic • Iron – Haber process • Vanadium (v) Oxide – production of sulphuric acid • Manganese (IV) Oxide (A) Inorganic • Iron – Haber process • Vanadium (v) Oxide – production of sulphuric acid • Manganese (IV) Oxide (B) Organic • Digestive enzymes • Metabolic enzymes (B) Organic • Digestive enzymes • Metabolic enzymes
  4. 4. • Enzymes are biological catalysts • which are mainly proteins that are produced by living cells. • Enzymes can be DENATURED by heating or by changes in the pH of the solution • E.g. Digestive enzymes, Metabolic enzymes (B) Organic Catalysts(B) Organic Catalysts
  5. 5. The function of enzymes • How is energy released from sugar (glucose)? • But how can our cells provide such high temperatures during cellular respiration to release energy from glucose?? Glucose + Oxygen  Carbon dioxide + Water + Energy Strong Heat
  6. 6. The function of enzymes Lower Activation EnergyLower Activation Energy • Activation Energy: Energy required to start a chemical reaction. Activation Energy with enzyme Activation Energy without enzyme Reactants (Substrate): (Glucose + Oxygen) Products: (Carbon dioxide + water) Energy Time
  7. 7. Enzyme-Catalyzed ReactionsEnzyme-Catalyzed Reactions (A) Synthesis of complex substances Anabolic Reactions • Protein synthesis • Photosynthesis • Conversion of glucose to glycogen • etc… (A) Synthesis of complex substances Anabolic Reactions • Protein synthesis • Photosynthesis • Conversion of glucose to glycogen • etc… (B) Breaking down of complex substances Catabolic Reactions • Digestion • Cellular Respiration • Detoxification in the liver and blood by catalase • etc… (B) Breaking down of complex substances Catabolic Reactions • Digestion • Cellular Respiration • Detoxification in the liver and blood by catalase • etc…
  8. 8. Enzyme-Catalyzed Anabolic ReactionsEnzyme-Catalyzed Anabolic Reactions Protein SynthesisProtein Synthesis • Synthesis of proteins from amino acids in the cytoplasm PhotosynthesisPhotosynthesis • Synthesis of sugar from carbon dioxide and water Glycogen formationGlycogen formation • Conversion of glucose to glycogen for storage.
  9. 9. Enzyme-Catalyzed Catabolic ReactionsEnzyme-Catalyzed Catabolic Reactions DigestionDigestion • Breakdown of complex food substances to smaller, simpler, more soluble and diffusible substances. • So that food can pass through the cell membrane and be absorbed into the bloodstream. • Enzymes involved – Hydrolases (e.g Carbohydrases, Proteases and Lipases)
  10. 10. Enzyme-Catalyzed Catabolic ReactionsEnzyme-Catalyzed Catabolic Reactions Cellular RespirationCellular Respiration • Oxidation of glucose by cells to release energy. • Many enzymes involved
  11. 11. Enzyme-Catalyzed Catabolic ReactionsEnzyme-Catalyzed Catabolic Reactions Detoxification in the liver and bloodDetoxification in the liver and blood • Conversion of toxic to non-toxic substances • Example: Conversion of toxic Hydrogen peroxide, H2O2 (a by-product of cellular respiration) to non- toxic water and oxygen by enzyme CATALASE
  12. 12. Classification of enzymes HydrolasesHydrolases • Enzymes that catalyzes hydrolytic reactions which involves the addition of water molecules to breakdown a complex molecule • Examples of hydrolytic reactions:  Digestion of carbohydrates, proteins & fats
  13. 13. Classification of enzymes HydrolasesHydrolases Types of Hydrolases Example(s) Substrate Carbohydrases Salivary and pancreatic amylase Starch Cellulase produced by some bacteria Cellulose Proteases Pepsin produced in the stomach Proteins Trypsin produced as an inactive form (trypsinogen) by the pancreas Proteins Erepsin produced by intestinal glands Polypeptides Lipases Pancreatic and Intestinal lipases Fats
  14. 14. Classification of enzymes Oxidation-Reduction EnzymesOxidation-Reduction Enzymes • Enzymes that are involved in cellular respiration. • Breaks down glucose to release energy, carbon dioxide and water
  15. 15. 15 Test yourself With questions in Textbook Page 75
  16. 16. 16 Test yourself – pg 75 - answers 1 An enzyme is a biological catalyst made of protein. It alters the rate of a chemical reaction without itself being chemically changed at the end of the reaction.
  17. 17. 17 Test yourself – pg 75 - answers 2 Yes, the statement is valid. Large, insoluble molecules such as carbohydrates, fats and proteins cannot diffuse through the cell surface membrane. They must first be converted into simpler, smaller substances, which are soluble and diffusible.
  18. 18. 18 Test yourself – pg 75 - answers 3 Meat contains mostly proteins, hence we can infer that this enzyme acts on proteins. The enzyme could soften meat by breaking down the proteins into peptides or amino acids.
  19. 19. 19 Test yourself – pg 75 - answers 4 A very rapid, violent chemical reaction would take place, with a thick layer of foam produced. Water and oxygen are produced when catalase in the red blood cells breaks down hydrogen peroxide.

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