Department of Pharmacology Faculty of Pharmacy Faculty of Biology (Experimental Biology Laboratory) Bioinformatics and Biostatistics
Genomics Center Real-time quantitative PCR Microarray experiments full range of genomics technologies including automatic DNA sequencing, gene expression array technologies, profiling, genotyping Bioinformatics Proteomics Center Metabolomics Center Other facilities provided for EIP purposes: HPLC and full analitical range of Mass Spectrometers Cell culture facilities Flow cytometry Confocal and deconvolution microscopy Imaging (PET, MRI, SPECT) scanning and transmission EM, atomic force microscopy Agricultural biotechnology - rural clusters Manufacturing - Biomanufacturing/ bioprocessing Informatics - life science applications linked to software/ IT clusters Research & Development – adjacent to universities with biotechnology / biomedical research focus
The earliest biotechnologists were probably the Sumerians who brewed their beer with yeast, thereby using living organisms. This use of biotechnology was not intentional. Today biotechnology is used in our every day lives, e.g. in the kitchen (food and dish liquid), in the bathroom (cosmetics and detergent) or whenever we are ill. In general you can say that biotechnology is the use of biological processes, organisms, or systems to manufacture products intended to improve the quality of human life. In recent years, biotechnology has expanded in sophistication, scope, and applicability. The science of biotechnology can be broken down into sub disciplines known as red, green, grey, white, and blue. Red biotechnology involves medical processes, for instance using organisms to produce new drugs, or using stem cells to regenerate damaged human tissues and perhaps re-grow entire organs. Green biotechnology applies to agriculture and involves such processes as the development of pest-resistant grains or the accelerated evolution of disease-resistant animals. Grey biotechnology is mainly focused on processes that digest existing toxic substances found in our environment. White biotechnology involves industrial processes such as the production of new chemicals or the development of new fuels for vehicles. Blue biotechnology encompasses processes in marine and aquatic environments, for example - controlling the proliferation of toxic water-borne organisms. LIS clearly focuses on red biotechnology - especially on cancer, inflammatory diseases, diseases of the central nervous system, and proteomics. This specialization is part of the overall concept and responds to the specific resources the Rhine-Main-area has to offer.
1- Manufacturing of the microarray: clone collection acquisition (+ sequencing), PCR amplification and insert verification, spotting, QC. 2- Experimental design and choice of reference: what to compare to what? 3- Target preparation (labeling) and hybridization 4- Image acquisition (scanning) and quantification (signal intensity to numbers) 5- Database building, filtering and normalization 6- Statistical analysis and data mining
PROF. DR DORU PAMFIL [email_address] HEAD of ILS Calea Manastur 3-5, 400372 Cluj-Napoca www.usamvcluj.ro INSTITUTE OF LIFE SCIENCE ILS
General Founded in 1869 Faculty of Agriculture Faculty of Animal Science & Biotechnology Faculty of Horticulture Faculty of Veterinary Medicine INSTITUTE OF LIFE SCIENCE (ILS) - 2007
Universities at Cluj-Napoca USAMV - ILS Grove Center University of Medicine and Phramacy “Iuliu Hatieganu” Technical University of Cluj-Napoca Faculty of Veterinary Medicine Institute of Cancer Research “dr. I. Chiricuta” Faculty of Horticulture Faculty of Animal Science & Biotechnology University of Babes Bolyai Faculty of Agriculture
Located in the heart of Transylvania
Full-time undergraduate programe, distance learning, e-learning and postgraduate program (master courses, joint doctoral)
Interdisciplinary Collaboration is a Hallmark of ILS Teaching & Research
Concept and Expansion of ILS INSTITUTE OF LIFE SCIENCE CLUJ-NAPOCA ILS – Innovation ILS – Research & Co-operation ILS – Facility ILS – Factors of Success ILS – Concept
Demand-Driven Process Approximately 90% Integration into the Workforce opportunity implementation results sustainability needs
Partnerships are Essential College Workforce Development Industry
Educational Development (PhD, MSc., Cert., Awareness)
Infrastructure for Universities Encourage multidisciplinary collaborative research Support faculty recruitment Provide venues for intellectual exchange Provide core facilities & equipment Move of IP from lab to commercialization Enhance high performance computing capabilities
POLICY MAKERS ACADEMIC PARTNERS ILS University of Medicine and Pharmacy University of Agricultural Sciences and Veterinary Medicine University of Babes Bolyai Technical University County Hospital of Cluj Institute of Oncology Institute of Public Health Institute of Public Health Veterinary State Direction EC NASR CNCSIS Start-up company Spin-off company PUBLIC PARTNERS
SAM assigns a score to each genes on the basis of the change in gene expression relative to the standard deviation of repeated measurements.
For genes with scores above a certain threshold (set by the user), SAM uses permutations of the repeated measurements to estimate the % of genes identified by chance = the false discovery rate (FDR).
Every cell in the body has the same genetic constitution. In cancer cells there is usually acquired aberrant DNA Development of “cancer genomics” to better understand the molecular basis of acquired genetic change Breast Ovary Prostate
TEAM WORK in prostate cancer at the IOCN-UMF-USAMV
Model of cell type specific regulation of target gene expression GLI X Y (tissue specific TFs) GLI GLI Ubiquitously expressed TF transcription transcription Common targets (e.g. Patched): Cell type specific targets (e.g. N-myc):
Doru PAMFIL, PhD — an accomplished professor of genetics, he has expertise in the management of many national and international projects and veteran of large, public research institutions.
Throughout his career he has focused on expanding undergraduate research opportunities and improving the education and professional experience of graduate students, with a focus on underrepresented groups.
Pamfil’s academic work focuses on Biotechnology (marker assisted selection, detection of GMOs, genetic transformation, micropropagation, microbiology). The GMOs Laboratory – CERTOMG - was included in EUROPEAN NETWORK OF GMO LABORATORIES (ENGL) in 2009
"The problem solving, systems thinking, and teamwork aspects of biotechnology can benefit all students, whether or not they ever pursue an scientific career," said Doru PAMFIL, Rector of the University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, head of INSTITUTE OF LIFE SCIENCES (LIS). "An biotechnology education and science curricula that does not include at least some exposure to biotechnology is a lost opportunity for students and for the nation."