Astec - Penguin AQ (APC/APM) Incubator User Manual - Supplied by RI UK and Ireland

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Astec - Penguin AQ (APC/APM) Incubator User Manual - Supplied by RI UK and Ireland.

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Astec - Penguin AQ (APC/APM) Incubator User Manual - Supplied by RI UK and Ireland

  1. 1. ASTEC CO., LTD User Manual - Penguin AQ APC-30D / APM -30D APC–30DR / APM-30DR APC-50D / APM -50D APC–50DR / APM-50DR Caution Please read this Manual / Instructions carefully prior to using the product for your own safety and proper use of the product. If not followed the manual, it may result in an accident or malfunctioning of the product.
  2. 2. INDEX of Contents 1. General Description 4 1-1 About this manual 4 1-2 Warranty and Liability 4 1-3 Warnings 4 1-4 Warning Labels 5 1-5 Earth Connection 8 1-6 CE Mark 8 1-6-1 Electromagnetic Compatibility 8 2. Bio Hazard 12 3. Important Notice 13 4. Copy Rights 13 5. Product overview 14 6. Specifications 15 7. Accessories 17 8. Dimensions 17 9. Diagram 18 10. Control Panel 19 11. Set up the Incubator 21 11-1 Location 21 11-2 Power 21 11-3 Power Code 21 11-4 Two Stacked Chambers 22 11-5 Earth Connection 23 11-6 Solenoid Valve 23 12. Shelves and Supports assembling 24 13. Water Jacket 26 14. Set Up Mode 27 15. Humidity 28 16. Gas Connections 29 17. Operations 30 17-1 Basic Settings 30 17-1-1 Temperature Setting 30 17-1-2 Over Temp Setting 30 17-1-3 30 CO2 Concentration Setting 17-1-4 Gas Selection Setting 30 17-1-5 31 O2 Concentration Setting 2
  3. 3. 17-2 Settings for Suspending Gas Controls and Alarms 31 17-2-1 CO2 ON/OFF Setting 31 17-2-2 O2 ON/OFF Setting 31 17-2-3 CO2 / O2 Alarm ON/OFF Setting 31 18. Alarms 32 18-1 ADD WATER Alarm 32 18-2 OVER TEMP. Alarm 32 18-3 CO2 Alarm 32 18-4 O2 Alarm 32 19. CO2 Concentration Check 33 19-1 CO2 Check prior to supplying CO2 gas to Incubator (CO2 ZERO) 33 19-2 CO2 Check 33 20. Sampling Mode Flow Chart of Setting Operations 21. Adjustment and Calibration 33 34 35 21-1 Temperature Adjustment (In-Chamber Temperature) 35 21-2 Displayed Temperature Adjustment 35 21-3 Door Temperature Adjustment 36 21-4 CO2 Zero Adjustment for AP series with T/C sensor 36 21-5 O2 Zero Adjustment 36 21-6 CO2 Adjustment 36 21-7 CO2 Adjustment Procedure 36 22. O2 Zero Adjustment and Calibration 38 22-1 Automatic CO2 Calibration 38 22-2 Manual CO2 Calibration 38 22-3 38 O2 Sensor failure Flow Chart of Adjustment and Settings 23. Calibration Settings 39 40 23-1 All format 40 23-2 40 CO2 span format 23-3 Cleaning and Disinfections 41 24-3-1 Cleaning 41 24-3-2 Disinfections 41 24. Trouble Shooting 42 25. 45 Regulator 26. Air Dust Filter Replacement Instruction 47 Factory Settings 48 3
  4. 4. 1. General Description 1-1 About This manual Thank you for purchasing ASTEC AP series incubator (Penguin AQ). The following instructions are for your own protection and safety use. Please read carefully before start using the instrument. Failure to observe these precautions can result in property damage or personal injuries. Make sure to keep this manual handy so that you can read this whenever you may need it. 1-2 Warranty and Liability We warrant the product you have purchased for one calendar year after the date of purchase. In case of any malfunctions caused by the manufacturer during this period of time, ASTEC will be responsible for repair or replacement of failed parts or even the instrument itself as circumstance allows for free of charge. However, this warranty excludes the following conditions. ♦ Any damages due to fire, earthquake, thunderstorm, and other catastrophic events and pollution and abnormal electrical supply. ♦ Any damages due to unduly repair, adjustment, calibration and modification. ♦ Any damages due to unduly usage or mishandling. ♦ Any damages caused by moving, dropping, transportation of the instrument. ♦ Replacement and repair of expendables and consumables. For service, please contact our agent that you have purchased the instrument from. Warranty is Valid only if the installation is done by people trained, in conformity to instructions provided in this manual. 1-3 Warnings 1. 2. 3. 4. 5. 6. Connect the instrument to a line plug according to local or county standards and to the specifications. Do not use the instrument for different purposes from those shown by the manufacturer. (See Chapter 1 for General Descriptions) Do not switch on the instrument without having waited at least 20 seconds after switching off. Do not open the instrument before reading the servicing specific conditions described in the manual. Follow instruction contained in the manual in case of changing any parts To move the instrument, at least two persons are required. We suggest to contact the distributor or directly to the manufacturer for servicing. 4
  5. 5. 1-4 Warning Labels Water Jacketed CO2/Multigas Incubator has an intrinsic danger due to the structure designed for the purpose. For the safe use of the instrument, the following mark is labeled on the instrument. Please use this instrument under a caution indicated on the labels. Warning Make sure of ground wire of power cord properly earthed. Power inlet on the back of the incubator is labeled with the Warning sticker. The following symbols are used in this manual. Warning This may cause serious or lethal injury. Caution This may cause serious injury or damage Warning, Caution Prohibited Important Note Warning Ventilate the room in which you use carbon dioxide with the instrument. It can harm your body without adequate air ventilation. Do not breath carbon dioxide directly. Do not place flammable objects in the incubator, such as alcohol, benzene, ethanol or propane gas. Especially when you use oxygen, do not use matches or lighters or cigarettes into the laboratory. Do not use this instrument outdoors. It could cause an electrical short or a lethal shock. Ask the manufacturer or your sales agent for the setting up. This will prevent from the events such as gas leaks, water leaks or any other unexpected problems caused by mis-handling. 5
  6. 6. The location where the instrument will be placed must be steady, firm and leveled. Do not leave this instrument in humid or wet conditions. It could cause an electrical short. When double-stack the incubators, use the optional brackets. The top incubator may fall by earthquake or other unexpected cause or accidents. Connect only to a suitable power source specifically assigned for the instrument(s) Using the instrument outside of Japan may require a transformer. Please ask the manufacturer or your sales agent. The power plug should be inserted all the way into the power outlet. Make sure that the power plug is clean and dust-free when used. Make sure that the instrument is securely earth-grounded. Do not earth the instrument to the gas pipe, water pipe and lighting rod. This will cause an electrical shock. Never use the flammable objects with the instrument. Make sure the type of gas and appropriate gas pressure are used with the instrument. All the gas connections with gas tube(s) must be firmly connected and tightened with cable-tie. Do not plug any wire or pin into gas ports, sampling port. Toxic, pathogenic and radioactive substances should be kept away from the instrument. During maintenance and cleaning, make sure that power off the instrument and unplug the power code. Do not take apart or modify the instrument without first-hand supervising. In case of malfunctions, turn off the power switch in the back of the instrument, and then, unplug the power cord. When the incubator is disposed, please be concerned for environment safety. 6
  7. 7. Cautions Pedestal, bench, desk or table where the instrument will be set up MUST be solid and leveled even to prevent leakage of the water in the Jacket. Using the correct power source specifically assigned for the instrument is extremely required. Do not unplug the power cable with soaked hands. In case any cuts or worn-out was found on the power cable of the instrument, stop using the cable and replace it with brand-new power cable. Do not operate the instrument with soaked hands. Do not put heavy objects or bottles that contain liquid. Do not get on the instrument. When close the inner and outer doors please hold the knob and handle in appropriate manner to avoid pinching your hands or fingers. Do not intentionally step on, bend or twist the power cable. Do not lean against the instrument. Do not lean against the door of the instrument, especially when it is open. Use appropriate gloves when cleaning the instrument, especially for cleaning the chamber. Do not inhale any chemicals, organic compounds, dust or moisture when cleaning. Power off and unplug the power cable when moving the instrument to different location. Remove the water from the water pan when moving the incubator to the different location. In case the instrument will be unused for a long period of time, remove the water in the water pan and dry out the chamber completely. Do not splash the water to the outside of the instrument especially on the top of it. The receiver of O2 sensor should be kept away from condensation. Used O2 sensor should be properly discarded in order to avoid contamination of the natural environment. 7
  8. 8. 1-5 Earth Connection This symbol shows at earth protection connections. 1-6 CE Mark Representative for CE: CGA Strumenti Scientifici Via Luca Giordano 7b 50132 Firenze – FI Tel. +39055571476 Fax +390555000889 E-Mail – support@cgastrumenti.com 1-6-1 Electromagnetic Compatibility 1-6-1-1 Requirements relating to electromagnetic compatibility The instrument must be installed and put in service according to the EMC information contained in this manual and any accompanying documents. You need to pay attention at the fact that other devices (particularly those portable radio and mobilei) can affect its comportment. The incubator should not be used near or above other apparatus. 8
  9. 9. 1-6-1-2 Electromagnetic Emissions The incubator is designed to work in the electromagnetic ambient here below specified. The incubator user must warranty the instrument to work in that ambient. Emission test Conformity Electromagnetic ambient - guide Emissions RF CISPR 11 Group 1 uses RF energy only for its internal functioning. Class B For this its RF emissions are very low and probably do not cause any interference in apparatus located near it. Armonic Emissions Class A IEC 61000-3-2 The incubator is suitable to be used in every ambient, including domestic and those connected Voltage fluctuations/flicker Conform directly to a public net supply with low voltage intended to supply buildings used for domestic IEC 61000-3-3 scopes. 1-6-1-3 Electromagnetic Immunity The incubator is designed to work in the electromagnetic ambient here below described. The user must warrantee to use the incubator in such ambient. Immunity test Test level IEC 60601 Conformity level Electromagnetic ambient – guide Electrostatic discharge ±6kV in contact ± 2kV in contact Floors must be in wood, concrete (ESD) IEC 61000-4-2 ± 8kV in air (± 2; ±4; ± 8)kV in air or ceramics. If floors are covered with synthetic material, relative humidity should be at least 30% A possible electrostatic discharge can cause only a display error, no significant error is generated. Transitory/fast electrical ± 2 kV for power supply lines ± 2 kV for power supply lines The quality of the line voltage subsequent ± 1 kV for I/O lines ± 1 kV for I/O lines should be that typical of a spikes commercial or hospital ambient. IEC 61000-4-4 Overvoltages ± 1 kV differential mode ± 1 kV differential mode The quality of the line voltage IEC 61000-4-5 ± 2 kV common mode ± 2 kV common mode should be that typical of a commercial or hospital ambient 9
  10. 10. Immunity test Test level IEC 60601 Conformity level Electromagnetic ambient – guide Voltage holes, short <5% UT <5% UT The quality of the line voltage voltage breaks on input (>95% hole in UT ) for 0,5 (>95% hole in UT ) for 0,5 should be that typical o fan supplì lines. cycles cycles commercial or hospital ambient. If IEC 61000-4-11 40% UT 40% UT (60% hole in UV) for 5 cycles (60% hole in UV) for 5 cycles 70% UT 70% UT (30% hole in UT) (30% hole in UT) for 25 cycles for 25 cycles <5% UT <5% UT (>95% hole in UT ) for 5 sec (>95% hole in UT ) for 5 sec 3 A/m 3 A/m Magnetic field at line the incubator user require a continuous functioning also during the line voltage break, we recommend to supply the incubator through a continuity group (UPS) or with batteries. Magnetic fields at line frequency frequency (50/60 Hz) should have levels characteristic of IEC 61000-4-8 a typical place of a commercial or hospital ambient 10
  11. 11. Immunity test Test level IEC 60601 Conformity level Electromagnetic ambient – guide Conducted RF 3 Veff 3 Veff The RF communication equipment IEC 61000-4-6 from 150 kHz to 80 MHz out of from 150 kHz to 80 MHz out of ISM (b) bands ISM (b) bands 10 Veff 10 Veff from 150 kHz to 80 MHz from 150 kHz to 80 MHz inside inside ISM(b) bands ISM (b) bands and mobile handsets should not be used longer anywhere near the incubator, cables, the distance separating recommended calculated using equation applicable to the frequency of the transmitter. Recommended separating distance d = 1,2 P from 80 MHz to 800 MHz d = 2,3 P from 800 MHz to 2,5 GHz where P is the maximum transmitter output power in Watt (W) as the transmitter manufacturer and d is the recommended distance in meters (m). The fixed RF transmitters field intensity, as determined by electromagnetic survey of the site could be less than the level of compliance in each frequency range. You can check interference in the vicinity of equipment marked with the following symbol: NOTE: Recommended separation distances between APM/APC incubators and radio communications equipment and mobile handsets The incubator is expected to operate in an electromagnetic environment in which irradiated RF noises are under control. The User of the incubator can help prevent electromagnetic 11
  12. 12. interference ensuring a minimum distance between appliances and mobile communications portable RF (transmitters) and the incubator as below recommended, in connection with a maximum power output of radio communication equipment. Distance Separation at the transmitter frequency (m) Maximum power output of from 80 MHz to 800 MHz from 800 MHz to 2.5 GHz transmitter specified (W) d = 1 , 2 P d = 2 , 3 0,01 0,12 0,23 0,1 0,38 0,73 1 1,20 2,30 10 3,79 7,27 100 12,00 P 23,00 NOTE: • At 80 MHz and 800 MHz the higher frequency interval is applied. • These guidelines may not apply to all situations. The electromagnetic propagation is affected dall'assorbimento and reflection on the part of structures, objects and people. The data reported in this chapter apply for the equipment used in the manner and with accessories specified in these instructions. The use of accessories other than those specified may result in increased emissions, or a decrease of immunity. 2. Bio Hazard This instrument may be used with radioactive products or pathogenic organism. If so, please be careful not to be exposed or infected by these substances. If the instrument have been used with radioactive products or pathogenic organism or any other substances which may be hazardous to human health, and it needs to be sent back for maintenance, it must be completely and thoroughly decontaminated before being sent back. Also, in case the instrument will be used with radioactive products or pathogenic organism or any other hazardous substances, please make sure to label the instrument as Biohazard. 12
  13. 13. 3. Important Notice *Read this section to the end prior to use ★ The AP series incubator requires separate CO2 and N2 (for Multi-Gas type - APM) cylinder. Premixed gas (ex. CO2: 5% O2: 5% N2: 90%) is prohibited. ★ A power outlet should be solely assigned to the incubator. Use of power strip or same kind of connections is not recommended. ★ Acid, benzene, soap, toothpaste are not recommended to use for cleaning. ★ Use of brush to any part of the incubator for cleaning is not recommended. ★ Do not wipe the plastic and rubber area with volatile liquid s uch as benzene. Use neutral detergent and wipe off completely with water. ★ Do not clean the incubator by poring water (especially on top panel) and wiping it off. ★ Pure distilled or even cleaner water is recommended for the humidification in the chamber in order to avoid any chance of contamination. Check the volume of remaining water at least once in a week. ★ Water for the humidification must be replaced or refilled as needed. The water should be pre-warmed to the same temperature as the set temperature of the incubator. ★ Pedestal, bench, desk or table where the incubator will be set up MUST be solid and leveled even to prevent leakage of the water in the Jacket. ★ Do not place flammable substances and volatile compounds in the incubator, such as alcohol, benzene, ethanol, adhesive or propane gas. Especially when you use oxygen, do not use matches or lighters and/or smoke cigarettes into the laboratory. ★ CO2 cylinder, which is equipped with siphon inside, cannot be used with the incubator. ★ Gas pressure to supply into the incubator such as CO2/N2/O2 should be maintained at 0.03~0.05MPa (4.35~7.25PSI). ★ In case the incubator will be kept powered off, remove the water for the humidification and dry out the chamber completely. ★ The incubator MUST be properly earthed to ground. ★ The AP series incubator is double structured with stainless-steal water tank jacket inside. Never attempt to make a hole or any kind of modification to the stainless tank. ★ Used O2 sensor should be properly discarded in order to avoid contamination of the natural environment. 4. Copy Rights All rights are reserved by ASTEC Co., Ltd. Revision, modification of the contents of this manual prior to permission is strictly prohibited. 13
  14. 14. 5. Product overview Design Having display and control panel on the front door, ASTEC AP series incubator has enables the small footprint while having water-jacket. Chamber Structure Main chamber is made out of Stainless steel, and is double insulated with water jacket and fiberglass insulation for secure temperature stability. Temperature Control PID control independently works for the water jacket main heater located on the bottom of the water jacket and door heater. Door Heater Ambient temperature significantly affects to the temperature distribution inside the incubator. Heater on the front door is adjustable to cope with the fluctuation of ambient temperature. Reducing the risk of Contamination The air circulation in the chamber is vertical flow, which is believed to be more anti-contamination. Stainless steel shelves, brackets and supports are removable without tools for easy cleaning. Stainless steel chamber with completely coved corners is easy to clean which enables the incubator to reduce the risk of contamination. Optional built-in HEPA filter, which can be replaced without tools, optimizes the air environment in the incubator and be preventive to contamination. Humidifier AP series utilizes Natural evaporation. CO2 Sensor IR sensor or TC sensor can be selected. Automatic and manual calibrations are available with AP series as standard. O2 Sensor (Multi-Gas: APM only) O2 sensor operates on galvanic battery. Automatic and manual calibrations are available with AP series as standard. Safety Functions Thermostat for temperature cut-off in case of deviation of water jacket temperature, Add water alarm for decreased water in the water jacket, and alarm functions for deviation of CO2, O2 are available to minimize the product loss. 14
  15. 15. 6. Specifications / CO2 Incubator APC-30D APC-30DR APC-50D APC-50DR Temperature Controller PID Independent electronic control Temperature Range 5C above ambient to 50C (122F) Control Accuracy ±0.1C Uniformity ±0.1C @37.0C (98.6F) Humidity Method Natural evaporation with humidity pan (Standard) RH Ambient to 95% @37.0C (98.6F) CO2 Control CO2 Sensor T/C Infra Red T/C CO2 Range 0~19.9% Readability 0.1% CO2 Control Infra Red ±0.1% O2 Control O2 Sensor N/A O2 Range N/A Readability N/A O2 Control N/A Constructions and Electrical Insulation 17.7” x 18.6” x 23.8” (inch) W320 x D300 x H330 (mm) W360 x D370 x H430 (mm) 12.6” x 11.8” x 19.0” (inch) 28kg / 61.7lbs 46kg / 101.4lbs (No water in the jacket) Weight W450 x D472 x H605 (mm) 12.6” x 11.8” x 19.0” (inch) Interior Dimensions W410 x D400 x H500 (mm) 16.1” x 15.7” x 19.7” (inch) Exterior Dimensions (No water in the jacket) Water jacket and fiberglass insulation Interior Volume 32 Liters/1.13 ft3 57 Liters/2.01 ft3 Water Jacket Volume 11 Liters 14 Liters Exterior Construction Cold- Rolled, Bonderized Steel, Powder coated Interior Construction SUS304, 100% coved, round corners Inner Door Tempered glass (5mm Thickness) SUS304, W280 x D265 x H7 (mm), SUS304, W338 x D332 x H7 (mm), 3 shelves standard, 7 maximum Shelves 3 shelves standard, 10 maximum Air Flow Direct airflow by Plastic wheel fan Operational Temp 5C~30C / 41F~86F Operational Humidity 30~80% RH Storage Temp -20C~50C / -3.9F~122F Power Voltage 110V 60Hz /230V 50Hz 15
  16. 16. 6. Specifications / Triple Gas (Multi Gas) Incubator APM-30D APM-30DR APM-50D APM-50DR Temperature Controller PID Independent electronic control Temperature Range 5C above ambient to 50C (122F) Control Accuracy ±0.1C Uniformity ±0.1C @37.0C (98.6F) Humidity Method Natural evaporation with humidity pan (Standard) RH Ambient to 95% @37.0C (98.6F) CO2 Control CO2 Sensor T/C Infra Red T/C CO2 Range 0~19.9% Readability 0.1% CO2 Control Infra Red ±0.1% O2 Control O2 Sensor Galvanic Battery O2 Range 1.0~89.0% Readability 0.1% O2 Control ±0.1% Constructions and Electrical Insulation 17.7” x 18.6” x 23.8” (inch) W320 x D300 x H330 (mm) W360 x D370 x H430 (mm) 12.6” x 11.8” x 19.0” (inch) 28kg / 61.7lbs 46kg / 101.4lbs (No water in water jacket) Weight W450 x D472 x H605 (mm) 12.6” x 11.8” x 19.0” (inch) Interior Dimensions W410 x D400 x H500 (mm) 16.1” x 15.7” x 19.7” (inch) Exterior Dimensions (No water in water jacket) Water jacket and fiberglass insulation Interior Volume 32 Liters/1.13 ft3 50 Liters/2.01 ft3 Water Jacket Volume 11 Liters 14 Liters Exterior Construction Cold- Rolled, Bonderized Steel, Powder coated Interior Construction SUS304, 100% coved, round corners Inner Door Tempered glass (5mm Thickness) SUS304, W280 x D265 x H7 (mm), SUS304, W338 x D332 x H7 (mm), 3 shelves standard, 7 maximum Shelves 3 shelves standard, 10 maximum Air Flow Direct airflow by Plastic wheel fan Operational Temp 5C~30C / 41F~86F Operational Humidity 30~80% RH Storage Temp -20C~50C / -3.9F~122F Power Voltage 110V 60Hz(US)/230V 50Hz (EU) 16
  17. 17. 7. Accessories List of standard accessories associated with AP series. antiseptic Side panel (Shelf support for right) 1 Manual 1 Side panel (Shelf support for left) 1 Plastic gas tube (3m) 1 Shelf brackets 6 Water filling silicon tube 1 Humidity Pan 1 Funnel 1 Ventilation ceiling 1 Anticorrosive solution 1 Shelves 3 Hexagonal wrench 1 3P adapter 1 Fill port 1 Knobbed screws for Ventilation Ceiling 2 8. Dimensions Figure 1 Dimensions 17
  18. 18. 9. Diagram Figure 2 Names of Components 1. Control Panel Displays the conditions of incubator and used for settings. 2. Adjustable Feet Adjust the physical level of incubator. 3. Outer Door Heated and insulated with fiberglass. Magnetic gasket. 4. Inner Door Inner door with sealed door trim. 5. 110V 60Hz(US)/230V 50Hz (EU) Power inlet 6. Circuit Breaker Protection for excess currency. 7. Recessed Power Switch 8. N /O2 Gas Port 2 9. CO2 Gas Port 10. Gas Sample Port 11. Front Fill Port Used to fill the water jacket. 12. Air Inlet Port Used to calibrate IR CO2 (DR) and O2 (APM) sensor with ambient air. 13. Access Port *Optional chamber thru-wall access port with rubber plug for 30L type Standard feature for 50L type Figure 3 Components in Chamber CO2 Sensor Sampling Port Screw Fan O2 Sensor* O2 Cal Tube* Monitor Temp Sensor N2 Gas Port* CO2 Gas Port * Multi-gas type only 18
  19. 19. 10. Control Panel APC-30DR/50DR (CO2 incubator) (1) (5) (2) (6) (3) (7) (8) (9) (10) (11) (4) (12)(13)(14) Figure 4 Control panel for APC 1. Temperature Display 2. CO2 Display 3. SET key 4. Alarm Silence key 5. Heating indicator Blinks when main heater being activated. 6. CO2 Inject indicator Blinks when CO2 being injected. 7. CO2 Setpoint Lights on when CO2 setting being modified. 8. Over Temp Setpoint Lights on when Over Temp setting being modified. 9. Temp Setpoint Lights on when Temperature setting being modified. 10. Programming Button Down cursor used when set parameter needs to be decreased. 11. Programming Button Up cursor used when set parameter needs to be increased. 12. Add Water Indicator Visual Alarm. Lights on when water in the water jacket needs to be refilled. 13. Over Temp Indicator Visual Alarm. Lights on when Over Temp alarm is activated. 14. CO2 Alarm Indicator Visual Alarm. Lights on when CO2 alarm is activated. Mode select, Set 19
  20. 20. 10. Control Panel -Continued- APM-30DR/50DR (1) (2) (6) (3) (7) (4) (8) (9) (10) (11) (12) (13) (14) (15) (5) (16)(17)(18)(19) 1. Temperature Display 2. CO2 Display 3. O2 Display 4. SET key 5. Alarm Silence key 6. Heating indicator Blinks when main heater being activated. 7. N2/O2 Inject indicator Blinds when CO2 being injected. 8. CO2 Inject indicator Blinds when CO2 being injected. 9. CO2 Setpoint Lights on when CO2 setting being modified. 10. Over Temp Setpoint Lights on when Over Temp setting being modified. 11. Temp Setpoint Lights on when Temperature setting being modified. 12. O2 Setpoint Lights on when O2 setting being modified. 13. N2/O2 indicator Selectable Gas indicator. Lights on when N2/ O2 selectable. 14. Programming Button Down Key used when set parameter needs to be decreased. 15. Programming Button Up key used when set parameter needs to be increased. 16. Add Water Indicator Visual Alarm. Lights on when water in the water jacket needs to Mode select, Set be refilled. 17. Over Temp Indicator Visual Alarm. Lights on when Over Temp alarm is activated. 18. CO2 Alarm Indicator Visual Alarm. Lights on when CO2 alarm is activated. 19. O2 Alarm Indicator Visual Alarm. Lights on when O2 alarm is activated. Figure 5 Control Panel for APM 20
  21. 21. 11. Set up the Incubator 11-1 Location l The place (bench, table or stand) where the incubator will be set up MUST be steady, firm and level. Make sure the surface that incubator will be placed by using adjustable feet as needed. To move the instrument, two or more persons are needed. l Please keep 25cm or more of clearance in the back and both side of the incubator. l Avoid placing an incubator where cold air drifts directly to the incubator such as air conditioning or air vent. It may cause condensation in the chamber. l All the selves, brackets, supports and surface of the chamber are thoroughly cleaned and sterilized with ethanol or autoclaves to make sure there is no contaminant on all these accessories. The laboratory itself introduces common source of contamination, incubator is designed to promote growth, through and constant cleaning are recommended. l Choose the place in the laboratory where there is no drastic change in temperature is expected to avoid condensation in the chamber. Preferably, incubator should be placed in a clean room environment such as biological clean room. 11-2 Power This incubator is designed to be operated with 110V 60Hz(US)/230V 50Hz (EU). Insert the supply cable in a plug with correct 110V 60Hz(US)/230V 50Hz (EU). The power plug connection must be independent, and provided with adequate earth connection. 11-3 Power Code If power outlet has 2 poles, avoid multiple connections using power strips. 21
  22. 22. 11-4 Two Stacked Chambers For space saving configuration, an incubator could be piled on the other. The optional hardware (a set of 2 brackets ③ and ④ in Figure 6) is needed to safely and steadily stack 2 incubators. ASTEC does not recommend stacking more than 3 incubators for safety reason. How to stack two incubators A. Remove the top panel [②] of the incubator which will be placed at the bottom by unscrewing the screw [①] in Figure 1. B. Securely mount the stacking hardware [③ and ④ in Figure 6] to the top frame of the bottom incubator with optional four of flat headed screws [⑤]. C. Remove the adjustable feet [⑥] from the incubator that will be placed on the top of the other. D. Slowly stack the top incubator onto the bottom incubator, and securely fix the incubator with optional doom headed screws [⑦] as shown in Figure 6. Figure 6 Stacking Incubators ⑤ Optional Flat headed screw ⑦ Optional Doom headed screw 22
  23. 23. 11-5 Earth Connection The incubator must be earthed to the ground in order to prevent electrical shock in case of deviation in electronic insulation. Do not earth the incubator to a water pipe or plastic make pipe. Do not earth the incubator to a gas pipe, telephone line or lighting conductor. 11-6 Solenoid valve The solenoid valve (s) will not start functioning for the first one minute after powering the incubator on in order to stabilize and initialize itself. 23
  24. 24. 12. Shelves and Supports assembling Firmly built stainless steel shelves and supports can be assembled and removed without using any tools for easy cleaning and adjustment. Clean the shelves, humidity pan and supports with 70% ethanol or inverted soap. All the interiors (shelves, supports, brackets) should be built in BEFORE the water jacket is filled with appropriate water. The water jacket expands when it is filled up. Figure 7 Figure 8 Figure 9 1. Figure 10 Mount the shelve support inner walls to both right and left side as indicated in Figure 7 and 8. 2. Mount the top part of the shelve system (ceiling) to the top part in the chamber, and hold it up with your hand as indicated in Figure 9. 3. Screw the ceiling onto the top inside of the chamber with knobbed screws as shown in Figure 10. Any part of the edge of the hole in the ceiling for the Fan must NOT touch the Fan. 24
  25. 25. 12. Shelves and Supports assembling -Continued- Mount the Brackets for the shelves to the holes on the support inner wall with the shelf assignment you need for the incubator. 3 shelves are come as standard. Figure 11 Figure 12 Figure 13 1. A bracket comes with two hooks on each. Mount the bracket to the support inner wall to the place you wish to mount a shelf as shown in Figure 11. 2. Slide the bracket into the slits that are made to the further holes as shown in Figure 12. 3. Hook the bracket down to the corresponding hole as shown in Figure 13. 25
  26. 26. 13. Water Jacket All the interiors (shelves, supports, brackets) should be built in BEFORE the water jacket is filled with appropriate water. The water jacket expands when it is filled up. 1. Take the plug out of the water port with supplied hexagon wrench as shown in Figure 14, and plug in the supplied connector to the water port as shown in Figure 15. Figure 14 2. Figure 15 Turn the power on and make sure the “ADD Water” indicator blinks with beeps. Wait for about 5 seconds until the incubator detects “No water in the jacket” to activate the indicator and beeps. 3. Press and hold the SET key for about 10 seconds, the incubator will be in “Set Up mode”. ? 4. Please see the page 26 for Set Up Mode Supply water into the water jacket from the water port with supplied hose or any other alternative hose until the beeping stops. 5. Supplied Anticorrosive solution must be supplied to the water jacket before the water jacket is full. Use RO water or cleaner water for the water jacket, however, water with high resistance such as ultra pure water would cause malfunction of the water level sensor in the jacket. If “Set Up mode” is not used when water being supplied, CO2 /O2 alarm would be activated. Appropriate resistance is needed for the water level alarm to work properly, make sure to provide Anticorrosive solution to the water jacket to gain resistance in the water. 26
  27. 27. 14. Set Up Mode The Set Up Mode is recommended for setting up the incubator for the first time, or setting it up again after long time unused period. Set Up Mode: Press and hold the SET key on the control panel for about 10 seconds. During the Set Up Mode, the Temp display shows the actual temperature and “SET” by turn. The Set Up mode enables the incubator to properly set itself up automatically. It automatically sets the pre-fixed parameters shown below after 12 hours from the time that Set Up mode is activated. During the 12 hours, incubator will shut CO2/O2 solenoid valves but warm up the water jacket in order to bring the temperature in the chamber to the pre-set temperature (37C). After 12 hours, it starts controlling the CO2/O2 to achieve the pre-set concentrations. Set Up Mode / Pre-fixed parameters Temperature 37 Celsius Over Temperature Set 38 Celsius CO2 Concentration Gas used with CO2 (APM only) O2 Concentration (APM only) 5.0 % N2 5.0 % * If you wish to change these settings, change them after Set Up mode is done after 12 hours. Before using Set Up mode, make sure there is NO CO2 gas in the chamber. The air in the chamber must be completely replaced with the ambient air. Humidity Water MUST be placed in the chamber when Set Up mode is in effective. ? See page 22. 27
  28. 28. 15. Humidity Water in the humidity water pan should be distilled or pure water and supplied in the pan to 1~2 cm from the bottom of the pan. Humidity water, because it is stagnant, can be common source of contamination within the incubator. So we strongly recommend that the humidity water should be replaced weekly or more often, and the water pan should be autoclaved or wipe down with a 70% of isopropyl solution or 70% ethanol. It is also recommended to warm up the humidity water to about 37C before it is pored into the pan for faster evaporation regain. When incubator is powered off more than 30 minutes, make sure to remove the water from the chamber. When incubator is powered back up, make sure there is NO humidity in the chamber. Humidity can harm CO2 and O sensor when 2 incubator is powered off. 28
  29. 29. 16. Gas Connections Gas port(s) is located on the back of the incubator as shown in Figure 16. Connect plastic hose(s) (supplied with the incubator) and firmly tie them up with cable ties. The other ends of the hose(s) needs to be connected to gas regulator of gas supply tank(s). Figure 16 Main Power Switch Gas Sampling port Circuit Breaker CO2 gas port * N2 gas port Power cord *APM series only 16-1 Gas Pressure The optimal gas pressure is 0.03~0.05 Mpa (0.3~0.5 bar / 4.35~7.25 psi) APC series (CO2 incubator) can only be connected with CO2 gas, and APM series (Multi-Gas type) can be connected with N2 or O2 gas of the user ’s choice. In case O2 gas is connected to the incubator, make sure to be cautious of the usage of fire as high concentration of O2 gas increases the combustibility of flammable substances. Excess gas pressure may cause the malfunction of the incubator. Be sure to select the correct gas type to your application of the incubator. ? See page 24 for gas selection for N2 and O2. Humidity and partial pressure of CO2 may cause the unstable control of high concentration O2 gas at the approximate range of 18~23%. 29
  30. 30. 17. Operations 17-1 Basic Settings Every operation and parameter can be changed by using <SET> key on the front control panel. Using <UP> and <Down> cursors allows changing the value for the settings or changing the parameters. After changing the value or parameter, if the operation is left unchanged about 7 seconds, it goes back to the normal display which indicates the current condition of the incubator. ? For detailed descriptions of the control panel, see the Control panel section on Page 12 and 13. 17-1-1 Temperature setting Press <SET> key once, the TEMP SET indicator lights on. Use <UP> and <DOWN> cursors to set the temperature to the value you wish to set. Press <SET> key once more, the control panel brings you to Over Temp Setting. If the control is left unchanged for about 7 seconds, it returns to normal display. 17-1-2 Over Temp Setting Press <SET> key until the Over temp set point lights on. Use <UP> and <DOWN> cursors to set the temperature to the value you wish to set. In case the incubator indicates the temperature in the chamber reaches the temperature you set here, it activates the Over Temp alarm. ? For detailed descriptions for Over Temp Alarm, see the Alarms section on Page 26. Press <SET> key once more, the control panel brings you to CO2 concentration Setting. If the control is left unchanged for about 7 seconds, it returns to normal display. 17-1-3 CO2 Concentration Setting Press <SET> key until the CO2 set point lights on. Use <UP> and <DOWN> cursors to set the CO2 concentration you wish to set. For APM series: Press <SET> key once more, the control panel brings you to the Gas selection (N2 or O2) Setting. For APC series: Press <SET> key once more, the control panel brings you back to the Temperature Setting. There is no Gas selection (N2 or O2) Setting on APC series. If the control is left unchanged for about 7 seconds, it returns to normal display. 30
  31. 31. 17. O p e r a t i o n s -Continued- 17-1-4 Gas Selection Setting For APM series only Press <SET> key until the N2/O2 indicator lights on. Use <UP> cursor to choose N2 and <DOWN> cursors to choose O2. Pressing <SET> key once more leads you to the next setting O2 concentration setting (APM series only). If the control is left unchanged for about 7 seconds, it returns to normal display. 17-1-5 O2 Concentration Setting Press <SET> key until the O2 set point lights on. Use <UP> and <DOWN> cursors to set the O2 concentration you wish to set. Pressing <SET> key once more leads you back to the Temperature setting. If the control is left unchanged for about 7 seconds, it returns to normal display. 17-2 Settings for Suspending Gas controls and Alarms By pressing <SET> key with <SILENCE> key pressed, the incubator enters the setting where Gas controls and Alarm function can be suspended by selecting ON or OFF. Function Suspending: 17-2-1 SILENCE + SET CO2 ON/OFF Setting In Function Suspending mode, Temperature display shows co2 and CO2 display show ON or OFF. The setting can be changed to ON or OFF by using <UP> and <DOWN> key. When the setting is set on OFF, the solenoid valve completely closes itself, and the incubator stops supplying CO2 gas. 17-2-2 O2 ON/OFF Setting For APM series only Pressing <SET> key once more when you are on O2 ON/OFF setting leads you to the O2 ON/OFF setting for APM series. Temperature display shows co2 and O2 display show ON or OFF. The setting can be changed to ON or OFF by using <UP> and <DOWN> key. When the setting is set on OFF, the solenoid valve completely closes itself, and the incubator stops supplying O2 gas. 17-2-3 CO2 / O2 Alarm ON/OFF Setting Press <SET> key in Function suspending mode until Temperature display shows ALA. Then, CO2 display shows ON or OFF. Use <UP> and <DOWN> key to change the setting. This function only suspends CO2 and O2 alarm but the ADD Water alarm for water jacket and Over Temp alarm are kept activated. 31
  32. 32. 18. A l a r m s 18-1 ADD WATER Alarm ADD WATER Alarm activates when the water in the water jacket decreased. The alarm gives out beeping sound that can be shut off by pressing <SILENCE> key. However, ADD WATER indicator keeps blinking until the water is actually refilled. ? For refilling water in the water jacket, see P20 Section Water Jacket 18-2 OVER TEMP. Alarm OVER TEMP. Alarm activates when the temperature inside the chamber reaches to the temperature that is set on OVER TEMP. Setting. The alarm gives out beeping sound that can be shut off by pressing <SILENCE> key. However, OVER TEMP setpoint indicator keeps blinking until the temperature is actually decreases. While the alarm is being activated, incubator partially shuts down the power to all the heaters for safety reason. 18-3 CO2 Alarm CO2 Alarm activates when CO2 concentration is off ±1.0% or more from the concentration that is set on CO2 concentration setting for 5 consecutive minutes. The alarm gives out beeping sound and CO2 Alarm indicator blinks. By pressing <SILENCE> key, beeping shuts down and the CO2 indicator turns off. The time delay for the alarm to activate is 5 minutes as a default configuration, however it can be modified at Adjustment B mode. ? See 18-3 O2 Alarm For APM series only O2 Alarm activates when O2 concentration is off ±5.0% more or less from the concentration that is set for O2 concentration setting for 60 minutes. The alarm gives out beeping sound and O2 Alarm indicator blinks. By pressing <SILENCE> key, beeping shuts down and the O2 indicator turns off. 32
  33. 33. 19. CO2 Concentration Check 19-1 CO2 Check prior to supplying CO2 gas to Incubator (CO2 Zero) Temperature and Humidity must be adequately prepared prior to supplying CO2 gas to incubator. 19-2 CO2 concentration must be 0.0% (ambient air). CO2 Check CO2 gas concentration must be checked frequently by CO2 analyzer for optimized use. If the displayed CO2 concentration differs from the concentration measured with calibrated CO2 analyzer, modify the concentration on the display of the incubator to the concentration measured with the analyzer. Before the Gas in the incubator is sampled out for concentration check, set the incubator to “Sampling Mode”. The Sampling Mode prevent CO2, N2 or O2 gas from coming into the chamber from the gas tanks in order to measure more precise concentration in the chamber. ? See the section 22 for Sampling Mode. ? See the section 23 for Adjustment and Calibration of Gas concentration. When you samples out some amount of Gas in the incubator, it sometimes activates false ADD WATER alarm. This is provoked by the gas sample port is capped with the Gas analyzer, and the analyzer pumps some gas out of the incubator and this causes negative pressure in the chamber. The false ADD WATER alarm will be deactivated when the pressure in the chamber regains positive pressure when gas sample port is open after sampling gas. 20. Sampling Mode For more accurate measurement of CO2 and O2 concentration, there should be none of such gas supplied during the measurement. The sampling mode allows the user to shut down the supply of gas for 3 minutes for reason above. Sampling Mode : <UP> key pressed for 5 seconds During the Sampling Mode, Temperature display shows SP and the current temperature by turns for 3 minutes. Concentration check must be done during this period. After 3 minutes, the Sampling Mode automatically cancels itself and goes back to normal function. 33
  34. 34. Flow Chart of Setting Operations Settings Settings for Suspending Gas controls and Alarm Normal Function Normal Function Silence + SET Pressed for 0.5 sec Temperature Setting SET OVER TEMP Setting SET CO2 Concentration Setting SET Pressed for 0.5 sec CO2 ON/OFF Setting SET O2 ON/OFF Setting SET Alarm ON/OFF Setting SET For APC series For APM series only Gas Selection Setting SET O2 Concentration Setting SET SET 34
  35. 35. 21. Adjustment and Calibration Adjustment / Calibration Mode is utilized for modifications to Temperature, CO2 and O2 concentrations by changing factory pre-set parameters as needed. The modifications should be made accordingly to adjust the environment in which the incubator is used. Some of the pre-set parameters should never be modified, as they are critical to overall performance of the incubator. These parameters are also indicated below here in this section. By pressing <SET> key for about 0.5 sec with <UP> and <DOWN> keys pressed and held, Adjustment / Calibration Mode will be activated. Adjustment / Calibration Mode: <UP> + <DOWN> Pressed and Held + SET pressed for 0.5 sec In Adjustment / Calibration Mode, the temperature display shows ST1 ~ST6 respectively as <SILENCE> key is pressed. However, ST2 , ST3 , ST4 and ST5 are all set by the factory and should not be changed or modified at all, except for C-5 under ST2 and E-4 under ST5 . ? See the flow chart of Adjustment / Calibration Mode on page 37 for further details. 21-1 Temperature Adjustment (In-chamber temperature) When Temperature display shows ST1 in Adjustment / Calibration Mode, press SET key to go to section A-1. You will see a value in this section. Increasing or decreasing the value here allows you to adjust the actual temperature in the chamber adjusted to the actual temperature. For example, when you need to increase the actual temperature by 0.2 C, increase the value shown by 0.2 using <UP> and <DOWN> keys. To achieve a precise adjustment of temperature, you need to use a calibrated thermometer to measure the actual temperature correctly. 21-2 Displayed Temperature Adjustment (In-chamber temperature) When Temperature display shows ST1 in Adjustment / Calibration Mode, press SET key until you see section A-2. Increasing or decreasing the value here allows you to adjust the displayed temperature on the control panel. For example, when you need to increase the displayed temperature by 0.2 C, increase the value shown by 0.2 using <UP> and <DOWN> keys. 35
  36. 36. 21-3 Door Temperature Adjustment When Temperature display shows ST1 in Adjustment / Calibration Mode, press SET key until you see section A-3. Increasing or decreasing the value here allows you to adjust the For example, when you need to increase the displayed temperature by 0.2 C, increase the value shown by 0.2 using <UP> and <DOWN> keys. 21-4 CO2 Zero Adjustment for AP series with T/C sensor This section is used for AP series incubator equipped with T/C sensor. Unused for APC/M-30DR series equipped with IR CO2 sensor. 21-5 O2 Zero Adjustment APM only When Temperature display shows ST1 in Adjustment / Calibration Mode, press SET key until you see section A-5 on the control panel. Increasing or decreasing the value here allows you to adjust the O2 ZERO on the control panel. APM incubator has a function called automatic O2 sensor ZERO calibration, so that this section will also be usually unused. However, the O2 sensor used for AP series is operated by a galvanic battery and it does have a life span of several years. When the battery deteriorates its out-voltage, this O2 Zero Adjustment can help up the voltage by gaining a few percentages (by increasing the value here). 21-6 CO2 Adjustment When Temperature display shows ST1 in Adjustment / Calibration Mode, press SILENCE key 5 times until you see section ST6 on the control panel. Pressing SET key allows you to go to section F1 ~ F8, however, F4 ~ F8 are used only for factory settings and should not be changed or modified by the users. 21-6-1 CO2 SPAN Adjustment F-1 When measured CO2 concentration is different from pre-set CO2 concentration (see page 24, Section 19-1-3 for CO2 Concentration setting), and it needs to be adjusted, change the value on F-1 to actual CO2 concentration by using <UP> and <DOWN> cursors. It will allow the incubator to automatically adjust the value to the measured value. 21-6-2 CO2 ZERO Manual Calibration F-2 This function allows the incubator to carry out manual ZERO Calibration. As a default, F-2 shows ON on the display. Change this to OFF with <UP> and <DOWN> cursors and wait for 10 second until the incubator starts manual calibration. 36
  37. 37. 21-6-3 CO2 ZERO Auto Calibration Interval F-3 The interval (days) for Auto Calibration can be changed here by modifying the number pre-set on F-3. Use <UP> and <DOWN> cursors to change the number (days). The Auto Calibration interval is set 10 days. Do not change the F-4 ~ F-8 as these setting are only used by the manufacturer. 21-7 CO2 Adjustment Procedure In case there is variation between displayed CO2 concentration and actually measured value, go through the below adjustment procedure to calibrate for the concentrations to match. When Temperature display shows ST1 in Adjustment / Calibration Mode, press <SILENCE> key 5 times until you see section ST6 on the control panel. On ST6, Press SET key until F-2 shows up on the Temperature display. On CO2 display, change displayed OFF to ON, Manual CO2 calibration will starts. When Manual CO2 calibration is finished, Press <UP> key for 5 second, Sampling mode will start and SP will show on the temperature display. Analyze the actual CO2 concentration with CO2 analyzer. Go to ST6 again and press SET key once to show F-1 on the temperature display. Change the value on F-1 to the actual CO2 concentration you have just measured. Leave the incubator for appropriate period until the CO2 concentration and humidity are 37
  38. 38. 22. O2 Adjustment and Calibration stabilized. And check the CO2 concentration again for double check. -APM only- As described in the section 22-5, the O2 sensor used on APM operates on galvanic battery and it has life span. It needs to be adjusted the voltage that effect the O2 concentration control. In case the battery is extremely run out, it must be replaced. There are two ways to calibrate the O2 sensor on APM series as described below. 22-1 Automatic O2 Calibration Automatic calibration works when the incubator is powered on and O2 setting is ON (see Page 29 for section 18-2-2: O2 ON/OFF setting). The automatic calibration performs no later than 12 hours after the incubator is first turned on. And it will perform once in every week. During the calibration, the O2 display shows the actual concentration of O2 and CAL by turn. 22-2 Manual O2 Calibration Manual O2 calibration should be carried out when O2 sensor is replaced, or the incubator has been not used for long period of time. 22-2-1 Manual Calibration Go to ST5 by pressing SET, <UP> and <DOWN> keys at the same time, go to E-4. You will see OFF on the display. Change it to ON by press <UP> cursor and leave it for a few seconds. The incubator starts manual calibration on O2 sensor. 22-3 O2 Sensor failure When the battery in O2 sensor wears out, O2 display shows the actual concentration and “---“ (bars) by turn, which indicates O2 sensor failure. The O2 sensor still controls the concentration even if the sensor failure is shown, but it is recommended to replace the sensor with new one at earliest possible. After replacing the sensor, make sure to do the Manual Calibration of the sensor. ? Please refer to the section 23-2-1 for Manual O2 sensor calibration. 38
  39. 39. Flow Chart of Adjustment and setting Normal Function Press <UP> + <Down> + SET all at once for few seconds SILENCE SILENCE SILENCE SILENCE SILENCE SILENCE Adjustment Mode Calibration Mode Calibration Mode Parameter Mode Calibration Mode Calibration Mode ST1 ST2 ST3 ST4 ST5 ST6 SET SET A-1 B-1 C-1 D-1 E-1 F-1 Chamber Temp Adjustment Water Jacket Temp CO2 Span Water Temp O2 Solenoid valve CO2 Span Adjustment Adjustment Control Setting Control (Open) Adjustment SET SET SET A-2 B-2 C-2 D-2 E-2 F-2 Displayed Temp Adjustment Displayed Temp Adjustment CO2 Solenoid valve Water Temp O2 Solenoid valve CO2 Manual Control (Open) Control Setting Control (Close) Zero CAL SET SET SET A-3 B-3 C-3 D-3 E-3 F-3 Door Temp Adjustment Door Temp Sensor CO2 Solenoid valve Water Temp O2 Solenoid valve CO2 Auto Adjustment Control (Close) Control Setting Control ON/OFF Zero Interval SET SET SET SET SET SET SET SET SET SET SET SET SET SET SET SET A-4 C-4 D-4 E-4 F-4 CO2 Zero Adjustment CO2 Solenoid valve Water Temp O2 Sensor Manual Control ON/OFF Anti-Reset Setting CAL Setting CO2 Solenoid Proportional Cycle Setting SET SET SET SET SET A-5 C-5 D-5 E-5 F-5 O2 Zero Adjustment CO2 Alarm Time Door Temp Off-Set Voltage CO2 Solenoid Setting Control Setting Adjustment Start Cycle Setting SET SET SET SET SET A-6 D-6 F-6 Ambient Temp Adjustment Door Temp CO2 Solenoid Control Setting Filter HIGH Setting SET SET SET D-7 F-7 Door Temp CO2 Solenoid Control Setting Filter LOW Setting SET SET D-8 F-8 Door Temp CO2 Zero Control Setting CAL Value 39 SET
  40. 40. 23. Calibration Settings SET Calibration setting ST 1 ST 2 ST 3 3 ST 4 ST 5 * A-1 0.00 B-1 XX.X C-1 X.X D-1 90.0 E-1 0.00 A-2 0.00 B-2 XX.X C-2 0.00 D-2 0.3 E-2 0.00 A-3 1.00 B-3 XX.X C-3 0.00 D-3 20.0 E-3 ON A-4 0.00 C-4 ON D-4 0.5 E-4 OFF A-5 -0.04 D-5 15.0 E-5* A-6 0.00 D-6 1.00 D-7 4.0 D-8 0.5 4 OFF ST 6 F-1 XX.X F-2 OFF F-3 10 F-4 5 F-5 -1.0 F-6 15 F-7 4 F-8 0.0 XXX represents that the v alues are unique to each individual incubator. 23-1 All format (Do not change it for daily operation) Power on while pressing UP/DOWN keys till it beeps 3 times for all formats. It will clear all setting of temperature, Overtemp, CO2 concentration, O2/N2 gas, O2 concentration, each mode setting. All the values will be displayed as shown the “Calibration setting” table above. XX shown in the table will be displayed 00. 23-2 CO2 span format (Do not change it for daily operation) Power on while pressing UP/SCIRENCE keys till it beeps 3 times for format CO2 Span. Once you set CO2 span adjustment value, no need to reset even on replacement of the CO2 sensor. 40
  41. 41. 23-3 Cleaning and Disinfections 24-3-1 Cleaning The outside of the incubator should be kept clean through with a soft cloth, water and soap. 24-3-2 Disinfections The product recommended for disinfecting is the Biocidal that can be used with the state in which we find ourselves following the instructions provided in the package. NOTE: You must use gloves as a routine precaution. Do not forget to turn off the incubator and disconnected the plug outlet from the network. Remove all shelves, media and the container of water to maintain humidity. Rub with a soft cloth soaked with Biocidal and dry carefully all surfaces. Be careful that no liquid comes in contact with the electrical parts. Reassemble in reverse order before turning on the incubator. Under no circumstances use following substances to clean The parts of stainless steel and others may be damaged: Sodium Nitride Acqua Regia Iodine Iron Chloride Sulphuric Acid 41
  42. 42. 24. Trouble Shooting Problem Check point Cannot power on. Is the power plug surely connected to the inlet? • Connect the plug into the inlet. Is the inlet supplied with electric power? • Check the breaker in your room or outside of the building. Is the breaker pulled out from the body? • Put the breaker in place and power on again. Warning! There are two fuses restorable: One is in the box on the back, and a second on the back next the main switch. Check both. Is the power switch on? • Breaker of the device is turned off even Check the switch and press ON of it. Is any liquid inside the device? • though power is on. Pull the power plug out immediately in the case of water splashing or pouring on the body, Contact Astec or any other distributors as soon as possible. If you keep using it, it may result in fire. Is the incubator placed in a rusty place? • Rust may stick to the power line to make it short circuit. Pull out the power plug immediately and contact Astec or distributors. It may cause fire if you keep using it. Breaker on the side of building goes down. Is the same power line divided into many devices? • Move each device to single lines. It may cause fire if you continue using without taking measure. Is the incubator connected into outlet with other machine that needs much power? • Connect the line of incubator to the single power inlet. The temperature inside the chamber cannot be stabilized. Is the fan inside the chamber moving properly? • Check if the fan is not touched to the duct or the sidewall of the chamber. 42
  43. 43. Is the glass window closed well? • Inside temperature cannot reach the set Close the window properly. Is the set temperature low? • temperature. Confirm the set temperature and set it to be [room temp + 5℃] if it is low. Is the room temperature too high? • The set temperature can be effective only it is not lower than [room temp + 5℃ ]. If the room temperature is too high, adjust it lower. CO2 concentration Is the fan inside the chamber moving properly? cannot be stabilized. • Check if the fan is not touched to the duct or the sidewall of the chamber. Are shelves positioned where they should be? • Disposition of the shelves causes bad circuit of the air inside and CO2 concentration cannot be stabilized. Put shelves in position. See p.10. Is the humidifying water enough? • Pour water into a tray immediately if there is not enough water. CO2 concentration Is the set CO2 concentration value low? does not become the • Confirm the set value. See p. 13. set value. Is the rest of gas enough? • Exchange with a new gas cylinder immediately if there is no gas in the cylinder. Is the regulator of CO2 gas cylinder adjusted to be 0.03 to 0.05 MPa? • Adjust the second pressure of the regulator if the pressure is too low. See p. 10. Is the exit valve of the regulator full open? • Open the exit valve fully on use. Is the CO2 gas line tube connected to the provided CO2 gas supply? • Confirm the tube connected to CO2 gas supply on use. 43
  44. 44. Is the gas line tube bended or squashed? • Repair the bended or squashed parts on use. Contact our distributor or us if the repair seems to be too hard or the tube is torn. CO2 alarm is beeping. Is the rest of gas enough? • Exchange with a new gas cylinder immediately if there is no gas in the cylinder. Is the regulator of CO2 gas cylinder adjusted to be 0.03 to 0.05 MPa? • Adjust the second pressure of the regulator if the pressure is too low. See p. 10. Is the exit valve of the regulator full open? • Open the exit valve fully on use. Is the CO2 gas line tube connected to the provided CO2 gas supply? • Confirm the tube connected to CO2 gas supply on use. Is the gas line tube bended or squashed? Repair the bended or squashed parts on use. Contact our distributor or us if the repair seems to be too hard or the tube is torn. ADD WATER alarm is The water level of the water jacket is low. Supply water from the beeping. water supply port at the front of the body. Refer the “Supplying water” on p. 11. ADD WATER alarm Is the water supplied Super pure water or Ion exchange water? does not stop during supplying water. • The water level us detected by electrical conduction. The sensor cannot response the water with high resist value (14MΩ) such as super pure water or ion exchange water. Supply antirust solution attached before the water full because it decreases resist value. 44
  45. 45. 25. Regulator General information This device adjusts high-pressure gas to low pressure to release the gas with regular pressured quantity. There is a high-pressure gas inside the regulator. Use the pressure-resistant device and inspect it regularly to keep normal condition. Preparation 1. The rust in regulator will cause troubles (gas leak, closure of gas path, etc). When installing to gas cylinder, clean the cap of the cylinder completely. On new plumbing, remove all of the rust, water, and lotion first. 2. When connecting regulator and gas cylinder, check that the gasket is inserted in position. If there is no gasket or the gasket is damaged, use preparatory one. 3. On installing regulator to gas cylinder, use right sized spanner and tighten firmly. Check gas leak after the installation. Operation 1. Turn the pressure-adjusting handle to “CLOSE” or “SHUT” (counterclockwise) before gas supply. Shut the valve on the exhaust to stop gas flow. Do not turn handle to “OPEN” by force when there is no gas in the chamber or the cylinder is not connected. 2. Open the valve of gas cylinder slowly and keep it to small amount until the gas fills with the chamber. After the gas full, open the valve fully. Avoid standing right in front of the pressure meter when opening or closing valve. 3. The regulator with high lateral pressure meter indicates the pressure at intake. 4. On the use of exhaustive pressure changeable regulator, the second pressure will be increased if the from pressure adjusting handle or screw turned to the direction of “OPEN”. The range of adjustable pressure is 60 to 70% of the maximum value of constant and lateral meter. 5. Open the valve of the exhaust to release gas. 45
  46. 46. Attention 1. Safety valve is preset at the safe pressure. 2. Check gas leak with soapy water or leak detective liquid. On the case of exhaust or damage of assembly, request our distributor or us for repair. 3. Our warranty excludes the following conditions even during the warranty period. l Damage due to fire, earthquake, thunderstorm, and other catastrophic events, and pollution and abnormal electricity supply. l Damage due to unduly repair, adjustment, and alteration. l Damage due to unduly usage or handling. l Damage due to the operation not mentioned in this manual. 46
  47. 47. Air Filter Replacement Instruction Please read through this instruction prior to replacing air dust filter. Air dust filters should be replaced at least once in every year. However, every laboratory must define and optimize the replacement interval for its own risk management according to the environment of laboratories. ♦ Air dust filters are NOT autoclavable. ♦ Sterilization with ethanol or method is not recommended since such methods do not remove dusts trapped in the filter. ♦ Incubators must be kept clean regularly even when air dust filter is in place. ♦ Do not touch air dust filters with bare fingers/hands. Aseptic gloves should be used. ♦ Air dust filters must be handled with care since it is somewhat fragile. ♦ Air dust filters must be stored protected from high temperature, humidity and dust. ♦ Do not immerse air dust filter in any kind of liquid. ♦ Air dust filters are disposable products, not reusable. [How to install Air dust filter] 1. Hook on the right side of the Air dust filters to the right steel clip as shown in Figure 1. 2. Gently push up the filter until the other side of the filter clips on the left steel clip as shown in Figure 1. 3. Make sure the filter is firmly placed and centered. Figure 1 [How to remove Air dust filter] Unclip the left side of the filter by gently pushing up the left steel clip. After replacing Air dust filter, you may need CO2 Figure 2 calibration due to the slight airflow change. 47
  48. 48. Factory Settings ST 1 ST 2 ST 3 A-1 0.00 B-1 C-1 A-2 0.00 B-2 C-2 A-3 1.00 B-3 A-4 0.00 A-5 A-6 3 ST 4 ST 5 * D-1 90.0 E-1 0.00 0.00 D-2 0.3 E-2 0.00 C-3 0.00 D-3 20.0 E-3 ON C-4 ON D-4 0.5 E-4 OFF -0.04 D-5 15.0 E-5 OFF 0.00 D-6 1.00 D-7 4.0 D-8 0.5 ST 6 F-1 F-2 OFF F-3 10 F-4 5 F-5 -1.0 F-6 35 F-7 12 F-8 0.0 4-6-15, Minamizato Shime Kasuya-gun Fukuoka 811-2207, Japan Tel: +81-92-935-5585 Fax: +81-92-936-6613 Email: info@astec-bio.com 48

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