Research Instruments - Pipette Challenge Competition Protocol

310 views
182 views

Published on

Research Instruments - Pipette Challenge Instructions. This documents explains the protocols of the pipette challenge competition. Good Luck!

Published in: Education
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
310
On SlideShare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
2
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Research Instruments - Pipette Challenge Competition Protocol

  1. 1. Pipette Challenge You can use either an Integra 3™ or a Integra Ti™ Micromanipulator for this challenge Starting position • Place RI pipettes (1 x Injection and 1 x holding) in their closed Twista-Pak™ on the relative side of the Integra™ stage • Micropipette holder’s (MPH) are to be placed in their holders in the secure position. They should be in the “up” location and clicked in the detent. • Place an empty Petri dish with a scratch in field of view but a little bit out of focus. • Have a 4x objective without spacer in place. Aim To have the holding and injection pipettes centred in position on the screen and with the tips in focus. Procedure 1. Start the stopwatch 2. Remove holding pipette from Twista-Pak™ 3. Remove MPH by winding it back with the axial drive until the “bald” part allows it to be withdrawn easily. Toolholder can either be swung forwards or can remain in position 4. Loosen MPH tip and carefully put pipette in 5. Secure and replace MPH securely in the toolholder 6. Focus the microscope on the scratch 7. Change to the 4x objective WITH spacer 8. Drive the MPHs forward with the black axial drive until they are on the screen 9. Rotate the MPH until pipette tip is horizontal 10. Focus the pipette tip using the red Fine Control Levers 11. Change back to the 4x objective WITHOUT spacer 12. Lower the pipettes carefully and centre using the silver coarse control levers 13. Fine-tune the focus on the pipette tips using the red fine control levers 14. Stop the clock

×