Big Brother Watching
Embryoscope is used for ALL PGD cases.
“Time lapse system can monitor any effects
(if any) on embryos biopsied via Laser or
acid and practioners”
Advances in PGD programme
Guy’s shifting towards TE Bx
Laser replacing acid tyrodes
Invested in the new Saturn 5 laser system
Hands free, programmable system
RI Saturn (3) laser system
Manual calibration and alignment challenging
Move embryo into target position physically
Laser using single shots via mouse/foot pedal
Difficult to keep steady
RI Saturn (5) laser system
• Calibration is quicker and simpler
• Automatic alignment using PC (simple
• Move the target into position for biopsy
• Mouse delivers pulse
• Multiple functions for biopsy
– Single, multiple or biopsy mode
• Easier to manipulate embryo
Embryo Biopsy by Laser
Single Cell Bx
D5 Biopsy (Trophectoderm)
TE Bx BC
Post TE Bx
Comparing laser with acid
Culture media use
minimise the effect
Calibration and QC Difficult
• Trophectoderm cells can be thermally damaged
and may not provide results (DNA damage).
• Laser is powerful and potentially embryo lethal.
Proper training and competency assessments
need to be in place (Guy’s data)
• Calibration and preventative maintenance
• EQAS – individual practitioners to send ‘typical’
biopsy videos for external assessment.
• Time-lapse analysis of all PGD cases to monitor
any effects of biopsy (ongoing)
Cost of Change
• Laser is a significant financial investment
• Previously centres use to make their own acid,
now commercially available
• PGD consortium data (shift from acid to laser)
• Long term picture – cost of laser offset against
– Reduced procedure times
– Reduced consumable costs
– Improved outcomes?
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