Nanostructured materials controlling biological processes

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This a presentation that was developed alongside the summer research program, Summer Institute in Math and Science (SIMS) at UCSB. The program duration was two weeks and also included students taking rigorous mock classes. SIMS was designed for incoming science students to help them succeed in the difficult path of science and engineering.

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Nanostructured materials controlling biological processes

  1. 1. Nanostructured Materials Controlling Biological Processes Team: Adrian Gusland, Basil Perkins, Diego Herrera, and Namgyal Sherpa Mentor: Dr. Won Hyuk Suh Advisor: Prof. Galen Stucky
  2. 2. What’s the Big Deal? <ul><li>Composition of microspheres makes them very biocompatible </li></ul><ul><li>Potential targeted drug delivery to individual cells </li></ul><ul><li>Current progress in the field of stem cell research </li></ul>
  3. 3. Project Goals <ul><li>Create porous microspheres for cargo transportation </li></ul><ul><li>Research delivery into cells </li></ul><ul><li>Investigate cell viability after delivery </li></ul>
  4. 4. Ultrasonic Spray Pyrolysis vacuum humidifier temp. regulator furnace cooling system bubbler flow meter airflow filter Ultrasonic fountain Suh, W. H.; Suslick, K. S. J. Am. Chem. Soc. 2005, 127 , 12007-12010.
  5. 5. The Etching Process <ul><li>Etch out SiO 2 with NaOH </li></ul><ul><li>Left with pores for cargo space </li></ul>SiO 2 TiO 2 pores NaOH
  6. 6. The Cleaning Process <ul><li>Remaining NaOH can not be present in finished product </li></ul><ul><li>Repeated wash cycles using: </li></ul><ul><ul><li>Water </li></ul></ul><ul><ul><li>Ethanol </li></ul></ul><ul><ul><li>Centrifuge </li></ul></ul>
  7. 7. Scanning Electron Microscopy 25 m 2 /g 53 m 2 /g Before etching After Etching SiO 2 pores
  8. 8. Average diameter of microspheres = 1.1µm 2 µm
  9. 9. Transmission Electron Microscopy <ul><li>T.E.M. shows density of particles </li></ul>Before etching After etching
  10. 10. Particle Internalization Optical Microscopy Analysis 20 x 100 x Microglia cells incubated with porous TiO 2 microspheres
  11. 11. Particle Internalization SEM analysis almost internalized internalization in progress Microglia cell grown with porous TiO 2 for 12h
  12. 12. Particle Internalization Fluorescent Microscopy Analysis arrow : cell undergoing mitosis (doubling) BV2 cell grown with porous TiO 2 for 12 hours Fixed using formaldehyde (HCHO) and DAPI nucleus stained
  13. 13. <ul><li>Creating porous microspheres increases surface area </li></ul><ul><li>Cytotoxicity of the spheres is low enough for cells to survive and replicate </li></ul>Conclusions
  14. 14. <ul><li>Tissue engineering </li></ul><ul><li>Targeted drug delivery </li></ul><ul><li>Chemical filtering </li></ul>Future Directions
  15. 15. Acknowledgments <ul><li>Dr. Won Hyuk Suh </li></ul><ul><li>Professor Galen Stucky </li></ul><ul><li>Mr. Se Yun Kim </li></ul><ul><li>Mr. Daniel Hazen </li></ul><ul><li>SIMS 2007 and faculty </li></ul><ul><li>National Science Foundation </li></ul><ul><li>California Nano Systems Institute </li></ul>

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