CYST
IODINE STAIN
Trophozoites, unstained Not characterictic
Trophozoites, stained Diagnostic: nucleus with large
central karyosome surround...
Iron Hematoxylin
stain
I.bütschlii trophozoite showing large blotchy
karyosome withing nucleus. Nucleus outline visible
sh...
Iron Hematoxylin
stain
I.bütschlii trophozoite - cell shape is more irregular
that Endolimax nana which is usually rounder.
Iron Hematoxylin
stain
I.bütschlii trophozoite - Binucleated cell
CYST
Nuclear membrane all but
invisible. Large glycogen vacuole seen
on right side of cell.
CYST
Large glycogen vacuole takes up most of
the cell's interior
CYST
binucleated cell
CYST
Concentrate (unstained)
CYST
BASKET OF FLOWERS, ESTER
BASKET
 The most common of the smaller
intestinal amebae
 usually encountered with about the same
frequency as in E. coli
 Siz...
Trophozoites, unstained Not charateristic
Trophozoites, stained Diagnostic: a nucleus with large
karyosome, generally with...
Trichrome stain
The nuclei in this mature, quadrinucleated cyst
have a large karyosome surrounded by a clear
halo; periphe...
Wet mount
examination of SAF-
fixed specimen
The nuclear characteristics of this trophozoite
are clearly visible
VIDEO PRESENTATION
THANK YOU FOR LISTENING! 
Idamoeba butschlii and e. nana
Idamoeba butschlii and e. nana
Idamoeba butschlii and e. nana
Idamoeba butschlii and e. nana
Idamoeba butschlii and e. nana
Idamoeba butschlii and e. nana
Idamoeba butschlii and e. nana
Idamoeba butschlii and e. nana
Idamoeba butschlii and e. nana
Idamoeba butschlii and e. nana
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Idamoeba butschlii and e. nana

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  • Trophozoites may be very difficult to recognize in an unstained concentrate as the nucleus is all but invisible.  The cyst is more obvious because of its large vacuole which, as above, appears as a void within the cell.  Iodine  can be added to stain the glycogen, making it more visible with the production of a dark brown colour.
  • Idamoeba butschlii and e. nana

    1. 1. CYST IODINE STAIN
    2. 2. Trophozoites, unstained Not characterictic Trophozoites, stained Diagnostic: nucleus with large central karyosome surrounded by a ring of small chromatin granules; or nucleus structure as in cyst Cysts, unstained Suggestive: large refractile body in cytoplasm; single nucleus Cysts, stained Diagnostic: basket nuclei or nuclei as in trophozoite; large glycogen vacuole
    3. 3. Iron Hematoxylin stain I.bütschlii trophozoite showing large blotchy karyosome withing nucleus. Nucleus outline visible showing no peripheral chromatin.
    4. 4. Iron Hematoxylin stain I.bütschlii trophozoite - cell shape is more irregular that Endolimax nana which is usually rounder.
    5. 5. Iron Hematoxylin stain I.bütschlii trophozoite - Binucleated cell
    6. 6. CYST Nuclear membrane all but invisible. Large glycogen vacuole seen on right side of cell.
    7. 7. CYST Large glycogen vacuole takes up most of the cell's interior
    8. 8. CYST binucleated cell
    9. 9. CYST Concentrate (unstained)
    10. 10. CYST BASKET OF FLOWERS, ESTER BASKET
    11. 11.  The most common of the smaller intestinal amebae  usually encountered with about the same frequency as in E. coli  Size range for both trophozoites and cyst is similar to that of E. hartmanni [(usual size, 4-12 µm) and cyst forms (usual size, 5-10 µm)], thus makes confusion in unstained preparations  Epidemiology is cosmopolitan  Colonizes the colon
    12. 12. Trophozoites, unstained Not charateristic Trophozoites, stained Diagnostic: a nucleus with large karyosome, generally with little or no peripheral chromatin Suggestive: Ovoid Cysts, unstained Suggestive: Ovoid Cysts, stained Diagnostic: 4 nuclei with large karyosome and little or no peripheral chromatin
    13. 13. Trichrome stain The nuclei in this mature, quadrinucleated cyst have a large karyosome surrounded by a clear halo; peripheral chromatin is absent.
    14. 14. Wet mount examination of SAF- fixed specimen The nuclear characteristics of this trophozoite are clearly visible
    15. 15. VIDEO PRESENTATION THANK YOU FOR LISTENING! 

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