Loading…

Flash Player 9 (or above) is needed to view presentations.
We have detected that you do not have it on your computer. To install it, go here.

Like this presentation? Why not share!

What is new on HHV 6,7,8 infections?, Henry J.C. de Vries

on

  • 918 views

 

Statistics

Views

Total Views
918
Views on SlideShare
918
Embed Views
0

Actions

Likes
0
Downloads
3
Comments
0

0 Embeds 0

No embeds

Accessibility

Upload Details

Uploaded via as Microsoft PowerPoint

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

What is new on HHV 6,7,8 infections?, Henry J.C. de Vries What is new on HHV 6,7,8 infections?, Henry J.C. de Vries Presentation Transcript

  • Henry J.C. de Vries Dermatology Academic Medical Centre University of Amsterdam The Netherlands
    • Until 1986, 5 herpes viruses
    • new human herpes viruses (HHV)
    • HHV-6 and -7
      • both members of the Roseolovirus genus of the β-herpesviruses.
      • T-lymphotropic but can infect other cell types
      • primary infections are associated with roseola infantum (a.k.a. exanthem subitum or 6th disease)
    • HHV lifetime infection
    • ubiquitous
    • reactivation
    • HHV-7 and HHV-6 reactivation associated with pityriasis rosea (Drago, 1997 and Yasukawa, 1999)
    • Debated
      • Innocent bystander?
      • Multiple agents?
    • Drug Reaction Eosinophilia and Systemic side effect Syndrome (DRESS)
    • HHV 6 reactivation (Deschamps 2001)
    • exanthema,hepatitis, colitis lymphadenopathy, eosinophilia, fever
    • EBV and amoxicillin associated drug rash in mononucleosis infectiosa
    C Goldberg, UCSD and Ascend Media Healthcare
  • Ascend Media Healthcare
    • Highest prevalence in over 50 year olds
    • Self limiting
    • Normally one episode
    • Association with HCV (Mokni 1991)
      • The epidemiological association is not strong (Imhof, 1997)
  • Electron microscopy of lichen planus lesional skin lichen planus lichen planus lichen planus reference herpes virus
    • Objective :
      • To find candidate herpes viruses associated with lichen planus.
    • Methods :
      • Lichen planus patients (pathologically confirmed, n=18)
      • Intra patient comparison of skin biopsies:
        • lesional vs. non-lesional
        • before vs. after remission
      • Inter patient comparison of skin biopsies:
        • psoriasis patients (lesional, n=11, and non-lesional, n=3)
        • normal skin (redundant after breast reduction, n=4)
      • DNA of HSV1 and 2, VZV, CMV, EBV (commercial PCR )
      • DNA of HHV 6, -7 and -8 (“in house” nested PCR)
  • * p=0,06, # p=0,05 p values calculated with McNemar test
    • All samples were free of HSV-1, HSV-2, VZV, CMV and HHV-8 DNA.
    • EBV DNA was detected in 2/15 lichen planus lesional samples.
    HHV7 HHV6 Lichen planus lesional non-lesional PBMC 11/18 (61%)* ,# 1/11 (9%)* 5/13 (38%) 0/18 (0%) 0/11 (0%) 2/13 (15%) Psoriasis lesional non-lesional 2/11 (18%) # 0/3 (0%) 1/11 (9%) 0/3 (0%) Normal skin 0/4 (0%) 0/4 (0%)
    • Immunohistochemical detection viral protein (HHV-7)
    • tegument protein pp85 (Advanced Biotechnologies)
    • positive cells/mm 2
    • (non) lesional lichen planus, psoriasis, normal skin
    lesional skin non-lesional skin de Vries et al. Br J Dermatol 154: 361, 2006
  • psoriasis lesional lichen planus normal skin non lesional lichen planus de Vries et al. Br J Dermatol 154: 361, 2006 -
  • lesional skin non-lesional skin CD123 positive cells(red), endothelial cells (blue) de Vries et al. Br J Dermatol 154: 361, 2006
  • HHV-7/BDCA-2 double staining HHV-7/CD-3 double staining lesional lichen planus lesional lichen planus de Vries et al. Arch Dermatol Res 299: 213, 2007
  • before treatment after remission de Vries et al. Arch Dermatol Res 299: 213, 2007
    • herpes virus like particles reside in lesional lichen planus skin
    • not HSV1, HSV2, CMV, VZV, HHV6 or HHV8 DNA
    • HHV-7 replicates in lesional lichen planus,
    • not in non-lesional lichen planus, psoriatic or normal skin
    • HHV-7 replicates in plasmacytoid dendritic cells
    • HHV-7 replication in lichen planus stops after remission
    • HHV-7 (subclinical) primo infection during childhood
    • HHV-7 reactivation in adult life
    • replication in basal keratinocytes/dermal lymphocytes
    • presentation (plasmacytoid) dendritic cells
    • inflammatory T lymphocytic response
    • destruction of the basal layer
    Skin Immune System, Bos JD ed. 3rd edition, 2005
    • viral “innocent bystander”
    • Koch’s postulates
    • g eographic variation in viral distribution
    • differences in laboratory protocols
    • virus-virus interactions
    • association with skin diseases?
    • or candidates in search of a disease?
    • Jan van Marle
      • electronmicroscopy
    • Jan Weel
      • virology
    • Fokla Zorgdrager and Marion Cornelissen
      • molecular biology
    • Daisy Picavet and Marcel Teunissen
      • immunohistochemistry