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Avian Influenza Survey in Migratory Birds

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This presentation was presented in front of 'Ornithology Course' Conducted By Abasaheb Garaware College, Pune and Ela Foundation in Year 2008. This Presentation gives Ornithological perspective of the …

This presentation was presented in front of 'Ornithology Course' Conducted By Abasaheb Garaware College, Pune and Ela Foundation in Year 2008. This Presentation gives Ornithological perspective of the Surveillance work, and field experiences during this work. Aditya Ponkshe and I jointly presented this lecture, as we were invited as Faculty at the Course.

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  • 1. Surveillance of Avian Influenza in Wild Birds 2007-2008 ELA FOUNDATION PRESENTATION BY PRANAV PANDIT ADITYA PONKSHE
  • 2. AI : Causes ?
  • 3. Hazards and Impacts of AI
    • 1. Crossing of Species Barrier
    • 2. Economical Impact
    • 3. Pandemics
  • 4. H5N1 in poultry & wild birds since 2003
  • 5. Recent Outbreaks of Avian Influenza H5N1 In India.
    • 1. Nandurbar & Dhule dists. Maharashtra (2005)
    • 2. West Bengal (2007)
    • 3. Meghalaya (2007)
    • 4. Assam and West Bengal (2008)
  • 6. How to Sample? Different Sampling Strategies
    • Monitoring wild bird deaths and sample collection of carcass tissue
    • Tracheal swabs
    • Cloacal Swabs
    • Fecal Samples
    • Environmental samples: Water, Mud etc.
  • 7.  
  • 8.  
  • 9. Materials
    • *Virus Transport Medium : VTM
    • Consists Buffers, antibiotics, pH indicator
    • *Maintaining cold chain
    • *Bio-safety Measures:
    • wearing personnel protective equipments: mask, gloves.
  • 10. Where to Sample?
    • 1200-1500 community size
    • Wetlands bearing congregation
    • Areas adjacent to poultry farms
  • 11. Fecal Sampling
    • Components of a bird dropping
    • Virus if present is seen in ‘pellet’ (GI excretion)
    Uric acid : (Renal excretion), ‘splash’ GI excretion, ‘Pellet’
  • 12. Collection
    • Collection of fresh feces from the dropping
    • 5 droppings from same species were pooled
    • Collection of other samples like pellets
    • Proper numbering and labeling
    • Transport on ice
  • 13. Sentinel Species
  • 14. Hirundinidae Columbidae Sturnidae Passeridae Bridge Species
  • 15. Characterization of Fecal matter of Different Species
    • For correct identification of dropping
    • Find Isolated flock of a single species and collect the fresh dropping from that particular place
    • Or observe a bird
  • 16. Parameters of Characterization A =Length of Pellet B= Length of splash C= Diameter of pellet a b c
    • Consistency
    • Colour
    • pH
    • Food remains
  • 17. Birds and their dropping characterization
  • 18.  
  • 19.  
  • 20. LARGER BIRD : LARGER DROPPING GULL SIZE 60 CM
  • 21. pH ranges from 4 – 6.5 towards acidic side ES’ s of migratory avian coastal species SANDWICH TERN LARGE CRESTED TERN LESSER CRESTED TERN RUDDY TURNSTONE GR. SAND PLOVER LS. SAND PL. CURLEW SANDPIPER LITTLE STINT
  • 22. Results (2007-08)
    • Total no. of samples collected : 3070
    • No. of species: 71
    • Resident species: 38
    • Migratory species: 32
    • Migratory and Resident: 1
    • Including, 26 Families
    • 51 Genera
    • NO H5N1
    • REO-VIRUSES IN SOME SAMPLES
    • OTHER VIRUSES : LPAI IN SOME SAMPLES
    • WORK GOING ON
  • 23. SEA Positive Elsewhere Positive Grey Heron Little Cormorant Little Egret Glossy Ibis Asian Openbill Eurasian Spoonbill Bar Headed Goose Northern Shoveller Common Teal Puple Swamphen Ruddy Shelduck Common Coot Mallard (Domestic) Common Redshank Eurasian Wigeon Green Sandpiper Spotbill Duck Rudy Turnstone Northern Pintail Black Winged Stilt Dunlin Barn Swallow Brown-headed Gull Yellow Wagtail Black-headed Gull White Wagtail Black Drongo Elsewhere 13 sp 7 families House Crow SEASia 15 sp 6 families
  • 24. Table : showing pH of some species Table: Localities under study with community size Locality Community Size Guhagar 3500-4000 Harne, Anjarale 1000 Yeralwadi 900 Shirwal 500 Kurkumbh, Kumbharga, Madanwadi 8000-9000 RuiChAtrapati, Visapur 800 Undri, Pilanwadi 500 Anand Nagar, Osade Wadki Kalamba Lake 900 Atigre Bawada 150 Krushna River 1200 Shirwal 200 Revas, Akshi 1500 Vantmuri 100 Hidkal, Yamkanmaradi 2000 Karambolim Lake 1000 Birds Scientific Names pH of Droppings Bar Headed Goose Anser indicus 6.5 Black Stork Ciconia nigra 6 White Backed Vulture Gyps bengalensis 6 Black Tailed Godwit Limosa limosa 4.5 Hoopoe Upupa epops 5.5
  • 25. Reference
    • 1. Guberti V. and Newman S. H.; Guidelines on Wild Bird Surveillance for Highly Pathogenic Avian Influenza H5N1 Virus1 , Journal of Wildlife Diseases, 43(3) Supplement 2007, pp. S29–S34 # Wildlife Disease Association 2007
  • 26. Acknowledgement
    • FOR AI WORK
    • National Institute of Virology, Pune.
    • Ela Foundation, Pune
    • FOR THE PRESENT TALK
    • Zoological Survey of India, Akurdi.
    • CERTIFICATE COURSE IN BASIC ORNITHOLOGY
  • 27. THANK YOU A Presentation by Aditya Ponkshe Pranav Pandit