Morphological Characterization of Rhinacanthus species in Sri Lanka presentation
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Morphological Characterization of Rhinacanthus species in Sri Lanka presentation

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Undergraduate final exam presentation, Department of Agricultural Biology, Faculty of Agriculture,

Undergraduate final exam presentation, Department of Agricultural Biology, Faculty of Agriculture,
University of Peradeniya

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Morphological Characterization of Rhinacanthus species in Sri Lanka presentation Morphological Characterization of Rhinacanthus species in Sri Lanka presentation Presentation Transcript

  • MORPHOLOGICALCHARACTERIZATION OF Rhinacanthus spp. IN SRI LANKA A.P.P.R AMARASINGHEDEPARTMENT OF AGRICULTURAL BIOLOGY FACULTY OF AGRICULTURE UNIVERSITY OF PERADENIYA
  • OutlineIntroductionObjectiveMethodologyConclusionFuture studiesReferences
  •  Family: Acanthaceae Rhinacanthus : 30 spp. (world) Two recorded spp. (Sri Lanka): Rhinacanthus nasutus (L.) Kurz Rhinacanthus polonnaruwensis Cramer A medicinal plant treats: -hypertension -tuberculosis (early stage) -snake venom -skin diseases
  • Composition: R. nasutus- Napthoquinones: rhinacanthin Lignans Flavonoids and phytosterols Effects: Cytotoxic, detoxicant, antifungal, antiviral, anti- bacterial, anti–cancer, anti-pyretic R. polonnaruwensis - not reported
  • TaxonomyKingdom: PlantaeDivision: MagnoliophytaClass: MagnoliopsidaSub class: AsteridaeOrder: LamialesFamily: AcanthaceaeSubfamily: AcanthoideaeGenus: RhinacanthusSpecies: Rhinacanthus nasutus (L.) Kurz
  • DistributionR. nasutus:  Sri Lanka  India  Indo- China  Introduced to: Madagascar, East Africa, Thailand, MalaysiaR. polonnaruwensis: Endemic
  • Examined SpecimensR. nasutus Anuradapura Polonnaruwa Matale Kandy Hambantota
  • Examined SpecimensR. polonnaruwensis Matale Polonnaruwa
  • Different morphologyR. nasutus collected from Ritigala - at BMARI (Bandaranaike Memorial Ayurveda Research Institute) shows a different floral morphology
  • Find out morphological variationswithin genus Rhinacanthus usingphenetic analysis
  •  Literature survey and studyherbarium specimens-familiarize with Rhinacanthus spp.-identify recorded variations
  • Field exploration Revised Hand Book to the Flora of CeylonInformation Herbarium samples Indigenous physicians
  • Field visits to:Polonnaruwa - Horiwila Matale - Nalanda Talakiriyagama Popham arboretum
  • Anuradapura - Habarana Gampaha -Yakkala Colombo-Maharagama
  • BMARI CollectionRitigalaKekirawaKumarakandaGirandurukotteIndigenousphysicianKataragama
  • Natural Medicinalforests gardens Plant Habitats Homegardens Roadsides
  •  Sample Rhinacanthus spp.Collection- live complete plantsPlant preservation- methanolFlower preservation- 70% ethanolLabelling - code number Prepare field notes Collection information, characters not in dry specimens Prepare dry specimens Dryers in National Herbarium
  •  Plant identificationCompare herbarium specimens Select and code morphological characters Quantitative characters: 68 Vegetative morphology: ruler, Vernier calliper, protractor Floral morphology: dissecting and stereo microscopes Qualitative characters: 20 Both categories: As binary variables
  • stempetioleveinsleaf bladeinflorescencecorollaandroeciumgynoecium
  •  Cluster analysis-Basic data matrix (MS Excel spreadsheet) - 88 characters (58 continuous,10 ratios, 20 discrete variables) - 123 OTUs (Operational Taxonomic Units)-Select continuous variables-Eliminate 8 characters-Second data matrix - 50 characters - 111 OTUs-Cluster analysis (SAS 6.12)
  • RESULTS AND DISCUSSION  Literature survey  Study herbarium specimens  Field exploration  Vegetative morphology  Cluster analysis  Floral characters
  • CONCLUSIONConfused plant by BMARI- R. polonnaruwensis4 significant variations within genus Rhinacanthus
  •  Include missing characters Another analysis method-discrete variables Other taxonomic information -biochemical -cytological -anatomical -molecular markers
  •  Cramer, L.H., 1993. A forest Arboretum in Dry Zone, Institute of fundamental studies, Kandy, Sri Lanka: Dassanayake, M.D. and Clayton W.D., 1987. A Revised Hand book to the Flora of Ceylon, Vol. 12 Oxford and IBH publishing Co. Pvt. Ltd, New-Delhi, India : Jayaweera, D.M.A. ,1981. Medicinal plants (Indigenous and Exotic) used in Ceylon, Part I, The National Science Council of Sri Lanka. Colombo: Judd, W.S., Campbell, C.S., Kellogg, E.A. and Donoghue, M.J., 2002. Plant systematics. phylogenetic approach, Sinauer Associates, Ind. Publishers, Massachusetts, U.S.A
  •  Padua, L.S.D., Bunyapraphastsara, N. and Lemmens , R.H.M.J. (ed) 1999, Plant Resources of South - East Asia ; Medical and poisonous plants 1.Backhuys, Leiden, vol1, no 12 : 431-433 Trimen, H.(1974). A Handbook to the Flora of Ceylon, III, Bishen Singh Mahendra Pal Singh, Deheradun, India. Periodical:286-287, 339-340. Vlas, J.D. and Vlas, J.(2008). Illustrated field guide to flowers of Sri Lanka, Mark booksellers and distributors (pvt) Ltd.(1):26.
  • ACKNOWLEDGEMENT Dr. D.S.A. Wijesundara, Director General, National Botanical garden, Peradeniya Dr. R. Karunagoda, Senior Lecturer, Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya Prof. A.P.G Amarasinghe, Professor, Institute of Indigenous Medicine, University of Colombo Bandaranayke Ayurveda Research Institute National Herbarium, National Botanical garden, Peradeniya
  • THANK YOU!
  • Key to the spp. (Cramer,1998):Leaves oblong- elliptic, flowers discreteat ends of secondary axes; corolla tubemarkedly curved from the middle to thethroat………….1. R. nasutusLeaves linear; flowers clustered at theends of secondary axes; corolla tubefaintly curved towards thethroat………….2. R. polonnaruwensis
  • (b.) (a) Herbarium specimens(a.) R. nasutus(b.) Unknown (c.)(c.) R. polonnaruwensis
  • Revised Hand Book to the Flora of Ceylon cited locations R. nasutus - Katharagama R. polonnaruwensis - Horiwila Popham arboretumLocations of herbarium specimens R. nasutus - Ritigala R. polonnaruwensis - NalandaOther locations - different
  • RNR.nasutus UN RPR.polonnaruwensis RP
  • (b.) (a.)Plant habits of:(a.) R. nasutus(b.) Unknown (c.)(c.) R. polonnaruwensis
  • (a.) (b.) (c.)Leaf morphology of:(a.) R. nasutus(b.) Unknown(c.) R. polonnaruwensis
  • R. nasutus UnknownR.polonnaruwensis R.polonnaruwensis
  • Unknown R. nasutusR. polonnaruwensis R. polonnaruwensis