Passaic County Community College
                                         BS 211 Genetics
Week                                     Genetics Lecture Topics

Section   Genes, Chromosomes and Heredity
   1     ...
BS 211-Genetics
                       Laboratory Topics

Week 1      Laboratory Topics

1           Lab Safety & Orientat...
                               ACADEMIC INTEGRITY POLICY

The College’s Academic Integrity Pol...
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Passaic County Community College


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Passaic County Community College

  1. 1. Passaic County Community College BS 211 Genetics Master Syllabus Course Credit Equivalents: Credit Hours – Lecture: 3 Credit Hours Laboratory: 1 Credit Hour Contact Hours – Lecture: 3 Contact hours Laboratory: 3 Contact hours Course Coordinator: Dr. Louis A. Scala Office Location: Room E503A @ Main Campus Office Phone #: (973) 684-6123 E-mail: Prerequisite: BS 101 Course Description: BS 211 is a lecture and laboratory course designed to discuss the structure & functions of genes, in both prokaryotic and eukaryotic cells, with more emphasis on eukaryotic systems. The overlying focus of this course is to discuss the key aspects of the “Central Dogma of Biology”. The expression of a gene into RNA and then a protein may be a very complex process that requires many steps. We will discuss many of the steps required to generate a protein from DNA sequences of genes, as well as the regulation of many of these steps. Topics that will be discussed include: a) chromosome structure and function, genomic regulation of gene expression, DNA replication and repair the control, b) the control of gene expression at the level of transcription, processing, and post-transcription. Additional learning objectives for the course will be to discuss the isolation of specific DNA sequences using recombinant DNA technology. A practical and systematic approach will be used to discuss the cloning of cDNA and gene segments. Student Learning Outcomes: Upon completion of this course, the student will be able to: 1. Identify the fundamental principles of chromatin structure, replication, repair and its role in heredity 2. Identify the different stages in which the expression of genes may be regulated and be able to explain the respective mechanisms of regulation 3. Demonstrate reading comprehension, critical thinking, and writing skills, by reading literature and writing reports that demonstrate awareness on how molecular genetics as science progresses due to on- going research 4. Perform laboratory experiments that will enhance knowledge of, develop hands-on skills and appreciation of methods in recombinant DNA technology 5. Explain the successes-as well as the dilemmas and difficulties in solving science problems through the use of the scientific method, in performing experiments and writing laboratory reports
  2. 2. Week Genetics Lecture Topics Section Genes, Chromosomes and Heredity I 1 Introduction to Genetics The Age of Genetics 2 Cell Cycle, Mitosis and Meiosis Chromosomal Basis of Inheritance 3 Mendelian Genetics Law of Segregation Law of Independent Assortment Section DNA: Structure, Replication & Variation II 4 Chromatin Structure & its Role in Gene Expression Modification of Histones & Nucleosomes DNA Replication & Repair 5 Semiconservative Replication Survey of DNA Damage & Mechanisms of Repair 6 Genetic Recombination, Mutation & Transposition Mechanisms for Varying DNA Sequence Section Expression & Regulation of Genetic Information III 7 Fundamentals of Transcription & Translation Following the Genetic Code 8 Transcriptional Regulation in Prokaryotes Organization & Regulation of Operons 9 Transcriptional Regulation in Eukaryotes Regulatory Sites & Transcription Factors 10 Post-Transcriptional Controls in Eukaryotes Processing & Transport Translational & Post-translational Controls Section Genomic Analysis IV 11 Recombinant DNA Technology Techniques of DNA Isolation, Manipulation, Cloning & Characterization 12 Fundamentals of Genomics & Proteonomics Identification & Gene Mapping Identification & Protein Analysis 13 Applications of Ethics of Biotechnology Revolutions in Agriculture, Medicine, DNA Fingerprinting
  3. 3. BS 211-Genetics Laboratory Topics Week 1 Laboratory Topics 1 Lab Safety & Orientation to the Course Measurements, Micropipetting, & Sterile Techniques 2 Experimental Meiosis & Recombination 3 Experiments in Mendelian Genetics using Fruit Fly 4 Bacterial Culture Part A & B 5 DNA Restriction & Electrophoresis 6 Recombination of Antibiotic Resistance Genes 7 Transformation of E. coli w/Recombinant DNA 8 Purification & Identification of Recombinant Plasmid DNA 9 Restriction Mapping of Lambda Chromosome 10 Polymerase Chain Reaction Amplification & Purification of Lambda DNA Fragment: Part A 11 Enzyme-Linked Immunosorbent Assay (ELISA) 12 Human DNA Fingerprinting: Detection of a Variable # Tandem Repeat (VNTR) Polymorphism by PCR Gel Purification of DNA Fragments 13 Southern Hybridization of Lambda Chromosome 14 DNA Sequencing
  4. 4. SCIENCE DEPARTMENT ADDENDUM ACADEMIC INTEGRITY POLICY The College’s Academic Integrity Policy is strictly enforced by the Science Department. All students are expected to familiarize themselves with the Academic Integrity Policy printed on page 25 of the 2009/2011 Academic Bulletin. The Policy clearly states that: “All members of the academic community at Passaic County Community College must maintain a constant commitment to academic integrity. Academic integrity is central to the pursuit of education. For students at PCCC, this means maintaining the highest ethical standards in completing their academic work. By completing their academic goals with integrity and honesty, students can reflect on their efforts with pride in their accomplishments.” The Academic Integrity Policy goes on to list examples of actions that violate the principle of academic integrity. These actions fall into one of two major categories: cheating and plagiarism. All students are expected to familiarize themselves with the definitions of cheating and plagiarism in the Student Code of Conduct printed on page 38 of the 2009/2011 Academic Bulletin. Please be advised that not being aware of College policies in no way excuses violations. In addition to the aforementioned policies, the unique activities, conditions, and potential dangers in science laboratories require that supplementary rules, regulations, and protocols are strictly followed. Therefore: 1. Students are expected to be familiar with and strictly abide by all laboratory safety procedures 2. No food or drinks are allowed in the laboratory 3. Backpacks, purses, and other personals must be placed underneath lab tables 4. All laboratory tables and counters must be cleaned upon arriving and again before leaving the lab 5. All dissection remains and chemicals must be discarded in appropriately labeled containers 6. All dissection tools must be washed, dried, and returned to designated areas 7. Prepared slides must be removed from microscopes and returned to correct trays 8. Microscopes must be returned to the proper shelf with cord wound and number facing out 9. All equipment and models must be returned to designated areas with parts properly attached 10. Unless otherwise informed by the instructor, students are not permitted to use and/or share electronic devices of any kind during examinations and classroom activities. 11. Unless otherwise informed by the instructor, students will complete and submit individual lab reports and assignments even if activities are conducted and data is collected in groups Please be advised that in accordance with the College’s Academic Integrity Policy, sanctions for violations “may be an academic sanction (reduced grade, a grade of “F” for the assignment, a grade of “F” for the course), or referral to the Student Affairs Office for a recommended disciplinary sanction (e.g., probation, suspension, or expulsion), or both.