Molecular Targets Development Program, CCR, NCI
Upcoming SlideShare
Loading in...5

Molecular Targets Development Program, CCR, NCI






Total Views
Views on SlideShare
Embed Views



0 Embeds 0

No embeds



Upload Details

Uploaded via as Microsoft PowerPoint

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
Post Comment
Edit your comment

Molecular Targets Development Program, CCR, NCI Molecular Targets Development Program, CCR, NCI Presentation Transcript

  • Molecular Targets Development Program, CCR, NCI
  • Advanced Biomedical Technologies CCR Clinical Program Molecular Targets & Molecular Oncology Genetics & Genomics Imaging & Biomarkers Integrated Systems Biology Immunology, Immunotherapy & Immunoprophylaxis HIV/AIDS Intramural/Extramural Translational Research at the CCR
  • Center for Cancer Research
    • 15 clinical branches
    • 38 basic sciences laboratories
    • > 330 principal investigators
  • A549 SNB19 MTDP HTS Data MTDP Mission Lab Bench Data
  • MTDP Mission MTDP
    • Collaborate with CCR investigators to develop and apply molecular target-specific assays
    • Screen the NCI natural products repository and other sources of chemical diversity
    • Identify and characterize target-specific compounds
    • Provide active compounds to collaborating labs for use as bioprobes
  • Target Consortium Molecular Target/Pathway Staff Scientists Lab Chiefs Postdoctoral Fellows PIs Proposal
  • Initial Feasibility
    • Quality control of reagents and cell lines
      • Reagent stability
      • Reagent specificity and binding characteristics
      • Cellular growth characteristics
    • Time frame of assay
      • Stable end point
    • Signal to noise ratio
    • Availability of appropriate controls
    • Availability of required equipment
    • Availability of sufficient reagents
  • Priority Areas
    • Screening Platforms
    • Natural Products
    • Protein Chemistry and Molecular Biology
    • Libraries
    • Imaging
    • Information Technology
  • Screening Platforms Enzymatic - FRET Protein-Protein Interactions - Time-Resolved Fluorrescence - Electrochemiluminescence Cell-based - Colorimetric - Fluorometric - Luminescent Cell-Imaging - GFP Translocation - GFP Expression
  • MTDP Ongoing Projects apoptotic synergy chemo-prevention breast cancer hypoxia response epigenetic control signal transduction multi-drug transport HIV inhibition p53 induced apoptosis Rationale cell adhesion Cripto 1 cKIT TRAIL cytoskeletal reorganization Ezrin AP1 signal transduction cMet ER signal transduction Smad 3 HIF-2  cell cycle control Polo-like kinase 1 HDAC/DNMT resistance to apoptosis RNAi/IGFR ABCG2 aberrant gene expression E2A/SCL RNaseH ER stress response gp78 MDM2/ ubiquitination Rationale Project Project
  • Current Libraries for Screening
  • NCI’s Natural Products Collections and Extract Repository Terrestrial Plants -Tropical and Subtropical Marine Invertebrates and Algae -Indopacific -Caribbean Selected Microbial Isolates -Cyanobacteria -Fungi > 65,000 Organisms > 170,000 Individual Extracts -Organic Solvent Extracts -Aqueous Extracts Collections Extract Repository