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  • 1. Pamela A. Padilla, Ph.D. Curriculum Vita Page 1 of 7 Pamela A. Padilla, Ph.D. Assistant Professor University of North Texas Departmental of Biological Sciences Denton TX 76203-5220 Tel: Office (940) 565-3614 Lab: (940) 891-6744 Email: PROFESSIONAL PREPARATION: • University of New Mexico Biology, With a Minor in Chemistry B.S. 1993 • University of New Mexico Biology (Molecular Genetics) Ph.D. 1998 • Fred Hutchinson Cancer Cell Biology, Genetics 1999-2002 Research Center, Seattle WA APPOINTMENTS: University of North Texas, Department of Biological Sciences Assistant Faculty (2002-present) • The research goal of my laboratory is to use genetic model systems (Caenorhabditis elegans and Zebrafish Danio rerio) to identify the molecular, developmental, genetic, and cellular responses to environmental stresses. We are particularly interested in: 1. Cell cycle changes in developing embryos exposed to oxygen deprivation, 2. Synergy between starvation, temperature and oxygen deprivation survival, and 3. Cellular structure responses to environmental changes. We conduct forward genetic and RNA interference screens to identify genes important for oxygen deprivation survival. The functional genetic approach (RNA interference) is used to identify genes required for oxygen deprivation survival. To date we have completed the RNAi screen for four of the six chromosomes and identified over 200 genes from, several gene classes, that are required for anoxia survival. We have also identified genes that are required for normal embryo development. Fred Hutchinson Cancer Research Center, Division of Basic Sciences • NSF Post-Doctoral Research Fellow in the Laboratory of Dr. Mark B. Roth (1999-2001) • Research led to the discovery that severe oxygen deprivation causes the zebrafish (Danio rerio) embryo and nematodes (C. elegans) to reversibly-arrest development and cell cycle progression, which we termed suspended animation. University of New Mexico, Biology Department • Graduate Student in the Laboratory of Dr. Margaret Werner-Washburne (1994-Dec. 1998) • Dissertation Title: Characterization of the SNZ and SNO gene families in Saccharomyces cerevisiae
  • 2. Pamela A. Padilla, Ph.D. Curriculum Vita Page 2 of 7 PEER REVIEWED PUBLICATIONS: T.Nystul, J. Goldmark, P. Padilla, M. Roth, 2003 "Suspended Animation in Caenorhabditis elegans Requires the Spindle Checkpoint" Science, 2003, Vol 302, p1038-1041. P.A. Padilla, T. Nystul, R. Zager, A. Johnson, M.B. Roth, 2002 “Dephosphorylation of cell cycle- regulated proteins correlates with anoxia-induced suspended animation in Caenorhabditis elegans”, Mol Biol Cell, May;13(5):1473-83. P.A. Padilla and M.B. Roth, 2001 “Oxygen deprivation causes suspended animation in the zebrafish embryo” Proc Natl Acad Sci U S A. 98(13): 7331-5. This article was highlighted in the "Editor's Choice" section of Science, Vol, 292. 29 June 2001, p2399. P.A. Padilla, E.K. Fuge, M. E. Crawford, A. Errett, M. Werner-Washburne, 1998. “The highly conserved, coregulated SNO and SNZ gene families in Saccharomyces cerevisiae respond to nutrient limitation,” J. Bacteriology, 180: 5718-5726. V.M. Peck, E.K. Fuge, P.A. Padilla, M.A. Gomez, M. Werner-Washburne, 1997. “Yeast bcy1 mutants with stationary phase-specific defects,” Current Genetics, 32:83-92. E.L. Braun, E.K. Fuge, P.A. Padilla, M. Werner-Washburne, 1996. “A stationary-phase gene in Saccharomyces cerevisiae is a member of a novel, highly conserved gene family,” J. Bacteriology, 178:6865-6872. PUBLICATIONS IN PROGRESS OF SUBMISSION FOR PUBLICATION: A. Mendenhall, B. LaRue, P. Padilla, “Identification of genes in the daf-2 pathway that mediate long-term anoxia and anoxia high temperature survival in Caenorhabditis elegans, In progress of submission to Genetics, 2005. V. Prabhu, J. Trejo, P. Padilla, “Temporal Cell Biological Changes Associated with Anoxia- Induced Suspended Animation in Caenorhabditis elegans” In progress of submission to Journal of Cell Biology, 2005. GRANTS AND FELLOWSHIPS: Current: • National Institutes of Health (RO1 GM069419-01A1) “Genetic and Cellular Analysis of C. elegans Exposed to Anoxia”, $625,000 direct cost. 2004-2009. Completed: • National Science Foundation Research Starter Grant, $50,000, 6/1/03 to 5/31/04, Analysis of ODS-1 in C. elegans Exposed to Anoxia (Project Summary Attached) • UNT Health Science Center, American Cancer Society Institutional Research Grant, $10,000, 01/01/03-12/31/03, Investigate the role the spindle checkpoint has in arresting C. elegans embryos exposed to anoxia
  • 3. Pamela A. Padilla, Ph.D. Curriculum Vita Page 3 of 7 • UNT Faculty Research Grant, $4,000, Genetic and Cellular Analysis of C. elegans Exposed to Anoxia Pending: • National Science Foundation, Acquisition of a Multi-user Confocal Microscopy Workstation and Development of Digital Pinhole Technology (PI: D. Root, Co-PI: H. Schwark, R. Dickstein and W. Burggren, B. Ayre, E. Dzialowski, P. Padilla) Grants and fellowships received prior to appointment at UNT: • National Science Foundation Minority Post-Doctoral Research Fellowship, $150,000 total 1999-2002 • National Institute of Health Minority Biomedical Research Support Stipend, 1997-98 • National Institute of Health P. Roberts-Harris Graduate Student Research Fellowship, 1993-96 • National Science Foundation RIMI Graduate Student Research Fellowship, 1993 • Howard Hughes Undergraduate Research Fellowship, 1992 SELECTION OF INVITED TALKS AND PRESENTATIONS: Talks and presentations: • National Academy of Sciences, Invitation to Seventh Annual Japanese-American Beckman Frontiers of Science Symposium, Poster Presentation: “Genetic and Cellular Approaches for Studying Oxygen Deprivation in C. elegans” P. Padilla, Irvine CA, 2004 (invited) • University of Texas at San Antonio, Department of Biological Sciences MBRS Seminar Speaker, “Suspended Animation: Anoxia Induced Cell Cycle and Developmental Arrest in C. elegans”, November 2003. • Texas Women’s University, Biology Department Seminar Speaker, “Anoxia Induced Cell Cycle and Developmental Arrest in C. elegans”, October 2003 • C. elegans International Meeting, UCLA, Oral Presentation, “Anoxia-Induced Suspended Animation in C. elegans”, July 2003 • C. elegans International Meeting, UCLA, Poster Presentation, “Spindle Checkpoint is Required for Suspended Animation”, July 2003 • UNT Health Science Center, Ft. Worth, Invited talk by Graduate Students, “Oxygen Deprivation in C. elegans and Zebrafish”, 2003. • American Society for Cell Biology Meeting, San Francisco, Poster Presentation, “Oxygen Deprivation in C. elegans- Is the Spindle Checkpoint Involved?” December 2002. • University of California Los Angeles, Invited talk by MBRS/MARC Undergraduate Students, “Oxygen Deprivation in C. elegans and Zebrafish” November 2002. Talks and presentations by graduate students in my laboratory: • American Society for Cell Biology, Washington DC, “Investigating the Signaling Pathway between Anoxia, Spindle Checkpoint Activation and Cell Cycle Arrest in C. elegans”, Vinita Prabhu, Jesus Trejo, Pamela A. Padilla
  • 4. Pamela A. Padilla, Ph.D. Curriculum Vita Page 4 of 7 • University of Houston Undergraduate Research Competition, “Investigating the effect of anoxia on chromosomes and microtubules in the C. elegans embryo”, Jesus Trejo, Vinita Prabhu, Pamela A. Padilla • University of Houston Undergraduate Research Competition, “Identification and characterization of a gene required for chromosome condensation”, Nicole Parker, Jeremy Holman, Pamela Padilla • Midwest C. elegans Meeting , “Analysis of C. elegans embryos exposed to anoxia”, Vinita Prabhu and Pamela Padilla, , 2004. • FASEB, “Metabolic Rate in Wild-type and hif-1 mutant Caenorhabditis elegans”, Tammy Chan, Warren Burggren, Pamela Padilla, 2004 Selection of talks and presentations prior to UNT appointment: • University of Washington, Guest Speaker for Scientific Journalism Class, Seattle WA, 2001. • Society for Developmental Biology 60th Annual Meeting, “Oxygen Deprivation Causes Suspended Animation in the Zebrafish Embryo,” P.A. Padilla, M.B. Roth, UW, Seattle WA, 2001. • 13th International C. elegans Conference, UCLA, Los Angeles CA “Oxygen Deprivation in C. elegans,” P.A. Padilla, T. Nystul, M.B. Roth, , 2001. • Yeast Genetics Conference, Collage Park, MD, “The Function of Snz1 and Sno1 Proteins in Yeast,” P.A. Padilla, M. Werner-Washburne, 1998. • United States Senate, Washington D.C., “The Need For the United States Senate to Continue Support of Basic Scientific Research,” P.A. Padilla (one of three graduate student speakers on behalf of the Science Coalition), 1996. TEACHING AND MENTORING EXPERIENCE: STUDENT RESEARCH TRAINING: • Ph.D. Students: o Alex Mendenhall, M.S. (Fall 2003 – Present) o Vinita Prabhu (Spring 2003 - Present) o Bobby LaRue (Spring 2004-present) o Michael Krebs (2005-present) • M.S. Students: o Michael Villarreal (Fall 2004-present) • Undergraduate Research Students: Nicole Parker, Jesus Trejo, Neil Stewart, Michael Villarreal, Jeremy Holman, Sheena Menezes, Randala Hamdan, Tammy Chan, Arman Ghandforoush, Joe Hamilton, McNair Scholar CoMentor (Jeremy Holman) Honors Research Advisor (Arman Ghandforoush and Tammy Chan), • TAMS Research Students: Jemma Alarcon (Semi-Finalist for Seimens scholarship), Desh Mohan SMART PROGRAM ADVISOR FOR HIGH SCHOOL SCIENCE TEACHERS: • Monica Amyette and Pamela Boyd were two high school teachers that worked two weeks in
  • 5. Pamela A. Padilla, Ph.D. Curriculum Vita Page 5 of 7 my lab. We taught them how to use model systems such as zebrafish and C. elegans to understand organism development. COURSE INSTRUCTION: • Teaching Evaluations Average: 1.5 on a scale of 1 to 5 with 1 being the highest and 5 being the lowest. • Genetics Biology 3451, Spring 2003 (99 students), Spring 2004 (159 Students)  This course is the traditional Genetics course required for undergraduates  Note: I implemented the use of important genetic model systems such (Caenorhabditis elegans and yeast Saccharomyces cerevisiae) into the laboratory section of Genetics Biology 3451. This will allow undergraduates to have exposure to two very important genetic model systems. • Eukaryotic Genetics, Biology 4005/5005, Fall 2003 (36 students), Fall 2004 (21 Students)  I developed this new course. The course focus and emphasis is eukaryotic genetic model systems, mapping of human genetic diseases, genome organization, chromosome structure, and cell cycle control. • Journal Club: Biology 5050, Developmental Physiology and Genetic Research Topics (Co Instructor with W. Burggren and E. Dzialowski) • Undergraduate Research, Biology 4900, Special Problems class for undergraduate students • Development and Cell Cycle (Biology 5005 Topics course) Fall 2002:Biology Topics course. • Guest Lecturer: Developmental Biology (Spring 2003), Biochemistry Vistas (Fall 2004) Instruction prior to appointment at UNT: • University of New Mexico: Guest Lecturer in Undergraduate Cell Biology, 1998 Teaching Assistant for Cell Biology, 1996 COMMUNITY SERVICE: • K-12 Science Fair Judge, Denton County, TX 2003 PROFESSIONAL AND COMMITTEE SERVICE: University Service: UNT Faculty Senate Committee on the Status of Women, (2002 – present) Departmental Service: Developmental Biology Search Committee (2005) Faculty Advisor for UNT Biology Department Undergraduate Club, Alpha Delta, (2002-present) McNair Scholar Research Judge (2003) Biology Department Ad Hoc Graduate Student Affairs Committee (Spring 2004) Biology Department Ad Hoc Beth Baird Gift Committee (Fall 2004) Graduate Student Research Day Faculty Advisor (2004-2005) Graduate Student Association Faculty Advisor (2003 – present) Host for Department of Biological Sciences Seminar Speaker: • Dr. Shane Kanatous, Fall 2003, University of Texas Southwestern Medical Center, Department
  • 6. Pamela A. Padilla, Ph.D. Curriculum Vita Page 6 of 7 of Internal Medicine; “A life without oxygen, the physiology of diving mammals” • Dr. Leon Avery, Fall 2004, UT Southwestern Medical Center, Department of Molecular Biology and Oncology, Dallas, Texas; "Feeding Behavior in Nematode, C. elegans". • Dr. Michael Herman, Fall 2004, Kansas State University, Division of Biological Sciences, Manhattan, Kansas; " Genetic basis for changes in nematode community composition in response to environmental cues". Professional Service: • Mid-West C. elegans Regional Meeting, Seminar Moderator (Summer 2004) • Journal Reviewer: Comparative Biochemistry and Physiology, 2003 • External Grant Reviewer: U.S. EPA- ORD-NCER, 2003 • Abstract Reviewer: SACNAS Meeting, 2003, 2004 • SACNAS Undergraduate Research Poster Presentation Judge, 2002, 2004 PROFESSIONAL ASSOCIATIONS: American Society of Genetics (ASG) Society for Developmental Biology American Society for Cell Biology (ASCB) Society for Advancement of Chicano and Native Americans in Science (SACNAS) RESEARCH COMMUNICATIONS: Prior to UNT appointment: Patent Application, 2001, “Methods for inducing reversible stasis”, P. Padilla and M. Roth. “Mysteries of an Ancient Gene,” M. Werner-Washburne, (P.A. Padilla, E.K Fuge, E. Braun), PBS Television Documentary on Scientific Research and Education, 1997. HONORS AND AWARDS: Prior to UNT appointment: • FASEB/NIH MARC Grant Writing Seminar Travel Award, 2002 • Phi Kappa Phi Honors Award for Outstanding Graduate Students, 1999 • American Society of Cell Biology, Minority Graduate Student Travel Award, 1995 • Graduate Student Poster Award, Biology Department UNM, 1995 • Society for the Advancement of Chicanos and Native Americans in Science Graduate Student Travel Award, 1995 • La Jolla Cancer Research Center (Burnham Institute) Graduate Student Travel Award, 1999
  • 7. Pamela A. Padilla, Ph.D. Curriculum Vita Page 7 of 7