Biology 201 – GENETICS –Spring 2004
Dr. Alice G. Lee Lecture: M, W, F 1:00- 2:05 p.m. Dieter Porter 208
Dieter-Porter 309 Lab: Tuesday, 2:30 - 5:30 p.m., Dieter-Porter 102
email@example.com Blackboard: BIO201
Outcomes: Students who have a basic understanding of genetics from General Biology will further their
knowledge of Mendelian, Molecular and Population genetics. Students will be able to quantitatively study the
genetics of different organisms, to collect and analyze data from various organisms, to use different statistical and
mathematical methods, to write scientific reports of their findings, and will have an appreciation of the technical
bioethical considerations of current genetic research and applications.
Date Topic Pages in Text
W 2/4/04 Chapter 1: Introduction 9-20
F 2/6/04 Chapter 2: Fundamentals of Mendelian Genetics 21-44
W 2/11/04 Chapter 3: Extensions and Applications of Mendelian Genetics 45-74
W 2/18/04 Quiz I
♣Water Plants in lab on 2/18 after class 75-104
and on 2/19 outside of class
F 2/20/04 Chapter 4: Chromosomes and Heredity
♣Thin Plants in lab after class
M 2/23/04 Chapter 5: Genetic Linkage 105-130
F 2/27/04 Exam I (Covers Mendelian Genetics)
M 3/1/04 Chapter 6: Chemistry of Genes 131-152
W 3/3/04 ♣Cross-Pollinate in lab (also need to do on 3/4/04)
F 3/5/04 NO CLASS: HHMI Mtg. on campus all day. Please visit poster session in afternoon.
M 3/8/04 Chapter 7: Replication and Recombination of Genes 153-180
W 3/10/04 Chapter 8: Transcription and Its Control in Prokaryotes 181-202
F 3/12/04 Quiz II
Chapter 9: Eukaryotic Gene Structure and Expression 203-236
F 3/19/04 Chapter 10: Translation 237-268
W 3/31/04 Exam II (Covers Molecular Genetics through Translation)
F 4/2/04 Chapter 11: Gene Mutation I: DNA Damage and Repair 269-294
W 4/7/04 Chapter 12: Gene Mutation II: Transposable Elements and Cancer 295-310
M 4/12/04 Chapter 13: Genetics of Bacteria and Phages 311-336
F 4/16/04 GUEST SPEAKER: Andy Lepisto, “Cancer Immunology”-tent. date
Chapter 14: Developmental Genetics
M 4/19/04 ♣Count Seedlings--in lab during class 337-362
W 4/21/04 and recount on 4/22/04 outside of class
F 4/23/04 Chapter 15: Gene Cloning and Manipulation 362-404
W 4/28/04 Quiz III
F 4/30/04 Bioinformatics Handout
M 5/3/04 Chapter 16: An Introduction to Population Genetics 405-430
F 5/7/04 Chapter 17: Extensions and Applications of Population Genetics 431-453
M 5/17/04, 2-5 p.m. Final Exam (Gene Mutation through Pop. Genetics and Laboratory)
Biology 201 – GENETICS –Spring 2004– Dr. Alice G. Lee
Laboratory Schedule: Tuesday, 2:30-5:30 p.m., Dieter-Porter 102 Assistant: Brooke Helfer
2/10/04 Safety Video (1 hour)
3 Principles of Probability
4 The Chi-Square Test (Parts IA and ID)
(You are responsible for doing part IC on your data from Investigations 2 and 3.)
2/17/04 2 ♣Plant Brassica rapa, F1 seeds.
Drosophila Dihybrid Crosses
(Adult flies will be removed in the morning so that virgin females can be obtained
2/18/04, ♣Water B. rapa with pipette from above—mandatory.
2/20/04 ♣Thin to one plant per cell—after lecture-- mandatory.
2/24/04 ♣Check plants and reservoir levels.
Check F1 from Drosophila crosses.
Remove parent flies (P1) from crosses.
11 Human Chromosomes
3/2/04 Mate F1's to produce F2.
♣Cross-pollinate B. rapa.
3/3/04 ♣Cross-pollinate B. rapa. –during lecture time.
3/4/04 ♣Cross-pollinate B. rapa and remove any unopened flower buds—outside of class--
3/9/04 8 Polytene Chromosomes from Drosophila Salivary Glands
Check F2 Drosophila. Remove F1 parents.
3/16/04 Determine phenotypes and sex of F2 from Drosophila crosses and record in Table 2.1.
**Report on Karyotype (Investigation 11) is due.
Note: Assistant will cross Sordaria next week (Part I or Investigation 13).
3/30/04 13 Genetics of Ascospore Color in Sordaria. An Investigatino of Linkage and
Crossing Over (Parts II, III, IV)
**Report on Investigation 2 (with Chi-Square analysis) is due.
♣Remove plants from watering system and allow pods to dry in paper bags on lighting
4/6/04 handout Chromosome 8: Gene Amplification of the Alu-TPA Gene by PCR
(Prepare samples and amplify)
♣Remove bags of pods from light bank.
4/13/04 handout Chromosome 8: Gene Amplification of the Alu-TPA Gene by PCR
(Electrophoresis and determine genotype for Alu insertion)
(Hardy-Weinberg Analysis of class data)
4/20/04 ♣Harvest seeds and sprout on filter paper.
Hand out PTC paper.
4/21/04 ♣Count seedlings—during lecture time.
4/22/04 ♣Recount seedlings-mandatory
Time TBD (Dr. Lee will arrange a time for you to come to the lab and work.)
4/27/04 24 Population Genetics: The Hardy-Weinburg Law
**♣B. rapa Chi-Square analysis and workbook due.
5/4/04 23 Polygenic Inheritance: Fingerprint Ridge Count.
**Questions from Chromosome 8 Gene Amplification are due.
5/11/04 **Gene Frequencies and Chi-Square on ABO data (Investigation 24) are due.
Review for Final Exam
Biology 201 – GENETICS –Spring 2004 (cont.)
Text: Weaver, R. F. and Hedrick, P.W. 1995. Basic Genetics. 2nd ed. William C. Brown
Publishers, Dubuque, IA.
Problems Book: Zwicker, K.2003. Student Study Guide in Genetics. 7th ed. William C. Brown
Publishers, Dubuque, IA.
Laboratory Manual: Mertens, T.R. and R.L. Hammersmith. 2001 Genetics Laboratory
Investigations, 12th ed., Prentice-Hall, Upper Saddle River, N.J.
Attendance: is expected in lecture and is required in lab. There will be no make up
laboratories. Ten points will be deducted from a student’s grade for every unexcused absence
from lab. If a laboratory activity is scheduled during lecture time and you miss class, it will be
considered a laboratory absence.
Laboratory Reports: You are responsible for completing all assigned investigations. Please
read the laboratory exercise before coming to lab. You should take notes during the laboratory in
your notebook and/or lab manual and use them for calculations and for studying. Reports of
many of the investigations will be turned in for a grade. Unless otherwise specified, you will
complete forms for data, answer questions and do calculation specified in the lab manual, rather
than turning in a full lab report. Please type onto the forms or word-process new tables onto
another sheet of paper.
Exam I 100 points
Exam II 100 points
Final Exam 200 points
Quiz I 10 points
Quiz II 10 points
Quiz III 10 points
Report on Investigation 2 25 points
Report on Investigation 11 25 points
Questions from Alu lab 25 points
Report on Investigation 24 25 points
B. rapa analysis 25 points
Total: 555 points
Dr. Alice G. Lee
Journals Received by W&J Library:
Journal of Heredity
Journal of Biological Chemistry
Books at W&J Library:
Drlica. Understanding DNA & Gene Cloning: A Guide for the Curious.
Tudge. The Engineer in the Garden: Genes and Genetics: From the Idea of Heredity to the
Creation of Life.
Lewin. Genes IV.
Bollag and Edelstein. Protein Methods.
Coombs. Dictionary of Biotechnology. 2nd ed.
Berg. Dealing with Genes.
McGrayne. Nobel Prize Women in Science.
Hoy. Insect Molecular Genetics.
Corcos. Gregor Mendel’s Experiments on Plant Hybrids.
Johnson. How Scientists Think.
Weismann. Democracy and DNA.
Kitcher. The Lives to Come: The Genetic Revolution and Human Possibility.
Vaughan. How Life Begins: The Science of Life in the Womb.
Aldridge. The Thread of Life: The Story of Genes
Books of Dr. Lee’s:
Lewin. Genes VII.
Maniatis. Molecular Cloning.
Watson. Recombinant DNA Technology, 2nd. ed.
Alberts. Molecular Biology of the Cell, 4th ed.
Bolsolver. From Genes to Cells.
Weaver. Molecular Biology, 2nd ed.
Campbell and Heyer. Genomics, Proteomics and Bioinformatics.
Videos of Dr. Lee’s:
Discovering Women (in Science)
Murder, Rape and DNA
Recombinant DNA Technology
The Building Blocks of Life
The Double Life of RNA (HHMI)
The Secret of Life (Biology Department)
The Immune System (HHMI)
Making Babies (PBS)
Clockwork Genes (HHMI)
A Harvest of Fear (PBS-NOVA)
CD-ROMS of Dr. Lee’s:
Explorations in Cell Biology and Genetics
An Electronic Companion to Genetics
Cells and Genes
Genetics Science Learning Center
Experiments and background information
Go to "Basic Genetics" at http://gslc.genetics.utah.edu/
Mendel Web: Experiments in Plant Hybridization
or use Yahoo to search for “MendelWeb” or http://morgan.harvard.edu
BioMolecules in the Classroom: Educational Molecular Visualization with Chime &
EXTOXNET - The Extension TOXicology NETwork (pl of 3)
The National Center for Biotechnology Information
BioChemNet. . . a directory of biology and chemistry educational resources
Medscape Home Page
Biology Place (Benjamin-Cummings)
Inheritance Patterns Analysis: Cotrix
The Biology Project (tutorials, animations and “Test Yourself”)
The National Biotechnology Information Facility
(tutorials and information, including ABO Blood Group tutorial)
The Darwin Awards (for your amusement)