Biology 201 – GENETICS –Spring 2005
Dr. Alice G. Lee Lecture: M, W, F: 1:00-2:05 p.m., Dieter Porter 208
Dieter-Porter 309 Lab: Tuesday, 2:30 - 5:30 p.m., Dieter-Porter 102
firstname.lastname@example.org Blackboard Course Site: BIO201
Office Hours: TBD
Outcomes: Students who have a basic understanding of genetics from General Biology will further their
knowledge of Mendelian, Molecular and Population genetics. Students will be able to quantitatively
study the genetics of different organisms, to collect and analyze data from various organisms, to use
different statistical and mathematical methods, to write scientific reports of their findings, and will have
an appreciation of the technical bioethical considerations of current genetic research and applications.
Students will make use of interactive cognitive genetics tutor computer programs to improve their skills
in solving genetics problems.
Date Topic Pages in
PART I: Introduction
W 2/2/05 Introduction to Course
Chapter 1: Overview of Genetics 1-16
PART II: Patterns of Inheritance
F 2/4/05 Chapter 2: Mendelian Inheritance 17-44
W 2/9/05 Chapter 3: Reproduction and Chromosome Transmission 45-74
F 2/11/05 Chapter 4: Extensions of Mendelian Inheritance 75-100
M 2/14/05 Chapter 5: Linkage and Genetic Mapping in Eukaryotes 101-136
F 2/18/05 Chapter 6: Genetic Transfer and Mapping in Bacteria and 137-165
M 2/21/05 Chapter 7: Non-Mendelian Inheritance
W 2/23/05 Chapter 8: Variation in Chromosome Structure and Number 193-226
F 2/25/05 Catch Up Day
M 2/28/05 Exam I (Covers Chapters 1-8)
W 3/2/05 lCross-Pollinate in lab (also need to do on 3/3)
PART III: Molecular Structure and
Replication of the Genetic Material
F 3/4/05 Chapter 9: Molecular Structure of DNA and RNA 227-252
M 3/7/05 Chapter 10: Chromosome Organization and Molecular Structure 253-278
Chapter 11: DNA Replication 279-206
W 3/9/05 PART IV: Molecular Properties of Genes
F 3/11/05 Chapter 12: Gene Transcription and RNA Modification 307-332
M 3/14/05 Chapter 13: Translation of mRNA 333-368
W 3/16/05 Chapter 14: Gene Regulation in Bacteria and Bacteriophages 369-398
F 3/18/05 TBA
M 3 /28/05 Chapter 15: Gene Regulation in Eukaryotes 399-432
W 3/30/05 Chapter 16: Gene Mutation and DNA Repair 433-461
F 4/1/05 Chapter 17: Recombination and Transposition at the Molecular 461-488
M 4/4/05 Catch Up Day
W 4/6/05 Catch Up Day
F 4/8/05 Exam II (Covers Chapters 9-17)
M 4/11/05 lCount Seedlings--in lab during class
and recount on 4/22/04 outside of class
PART V: Genetic Technologies
W 4/13/05 Chapter 18: Recombinant DNA Technology 489-520
F 4/15/05 Chapter 19: Biotechnology 521-550
M 4/18/05 Chapter 20: Structural Genomics 551-576
W 4/20/05 lCount Seedlings--in lab during class time
and recount on 4/21 and 4/22 outside of class
F 4/22/05 Chapter 21: Functional Genomics, Proteomics and Bioinformatics 577-604
PART VI: Genetic Analysis of Individuals and Populations
M 4/25/05 Chapter 22: Medical Genetics and Cancer 605-632
W 4/27/05 Chapter 23: Developmental Genetics 633-664
F 4/29/05 Chapter 24: Quantitative Genetics 665-694
M 5/2/05 Chapter 25: Population Genetics 695-722
W 5/4/05 Chapter 26: Evolutionary Genetics 723-749
F 5/6/05 Catch Up Day
M 5/9/05 Catch Up Day
TBD Final Exam (Covers Chapters 18-26 and Laboratory)
Biology 201 – GENETICS –Spring 2005– Dr. Alice G. Lee Assistant: Alicia Bitterice
Laboratory Schedule: Tuesday, 2:30-5:30 p.m., Dieter-Porter 102 for lab work
Vilar 222 for Genetics Cognitive Tutors
Date Investigation Tasks to Accomplish
2/8/05 Genetics Tutor: Mendelian Transmission 1: Forward Modeling
Cognitive Tutors Tutor: Mendelian Transmission 2: Experimental Design and
Villar 222 Interpretation
2/15/05 lPlant Brassica rapa, F1 seeds.
Investigation 3 Principles of Probability
Investigation 4 The Chi-Square Test (Parts IA and ID)
(You are responsible for doing part IC on your data from the B. rapa
2/16,05 lWater B. rapa with pipette from above—mandatory.
2/17/05, Some can be done after lecture; the rest must be done outside of class.
2/18/05 lThin to one plant per cell—after lecture-- mandatory.
2/22/05 Genetics Tutor: Pedigree Probability Analysis with Explanations
Cognitive Tutors (pre- and post-tests)
lCheck plants and reservoir levels.
Investigation 11 Human Chromosomes – pictures for karyotype analysis will be handed
3/1/05 Genetics Tutor: Gene Interaction
Villar 222 lCross-pollinate B. rapa.
4:30 p.m. - D-P
3/2/05 lCross-pollinate B. rapa. –during lecture time.
3/3/05 lCross-pollinate B. rapa and remove any unopened flower buds—outside
of class-- mandatory.
3/8/05 Genetics Tutor: Progeny Prediction
Cognitive Tutors (pre- and post-tests)
3/15/05 Genetics Tutor: Tetrad Analysis
Villar 222 **Report on Karyotype (Investigation 11) is due at 2:30.
3/29/05 Genetics Tutor: Three-Factor Cross
Villar 222 lRemove plants from watering system and allow pods to dry in paper bags
4:30 p.m. - D-P on lighting apparatus.
4/5/05 handout Chromosome 8: Gene Amplification of the Alu-TPA Gene by PCR
hf (Prepare samples and amplify)
lRemove bags of pods from light bank.
4/12/05 handout Chromosome 8: Gene Amplification of the Alu-TPA Gene by PCR
(Electrophoresis and determine genotype for Alu insertion)
(Hardy-Weinberg Analysis of class data)
4/19/05 lHarvest seeds and sprout on filter paper.
Hand out PTC paper.
4/20/05 lCount seedlings—during lecture time.
4/21& lRecount seedlings-mandatory (time to be arranged by Dr. Lee.)
4/26/05 Investigation 24 Population Genetics: The Hardy-Weinburg Law
**lB. rapa Chi-Square analysis and workbook due at 2:30.
4:30 p.m. Guest Speaker: Elizabeth Jenkins (W&J Biology 2004), “Genetic
5/3/05 Investigation 23 Polygenic Inheritance: Fingerprint Ridge Count.
**Questions from Chromosome 8 Gene Amplification are due at 2:30.
5/10/05 **Gene Frequencies and Chi-Square on ABO data (Investigation 24)
are due at 2:30.
Review for Final Exam
Visit Natural Science and Math Division Poster Session
Biology 201 – GENETICS –Spring 2005 (cont.)
Text: Brooker, R.J. 2005. Genetics Analysis and Principles, 2nd edition, McGraw-Hill, New York, NY.
Study Guide: Brooker, R.J. and M Windelspect. 2005. Student Study Guide/Solutions Manual for use
with Genetics Analysis and Principles, 2nd edition, McGraw-Hill, New York, NY.
Laboratory Manual: Mertens, T.R. and R.L. Hammersmith. 2001 Genetics Laboratory Investigations,
12th ed., Prentice-Hall, Upper Saddle River, N.J.
Genetics Cognitive Tutor Program: (from Carnegie Mellon University). We will be serving as a user
site for this program this semester. Work will be completed in laboratory from the program to help you
master the process of doing different types of genetics problems. You will have the opportunity to
rework problems after the exercises are completed after the programs are analyzed and re-set. The
programs will help you with lecture and laboratory exams and reports, but will not cover every aspect of
the course. You will also receive points for your participation in this activity.
Attendance: is expected in lecture and is required in lab. There will be no make up laboratories. Ten
points will be deducted from a student’s grade for every unexcused absence from lab. If a laboratory
activity is scheduled during lecture time and you miss class, it will be considered a laboratory absence.
Laboratory Reports: You are responsible for completing all assigned investigations. Please read the
laboratory exercise before coming to lab. You should take notes during the laboratory in your notebook
and/or lab manual and use them for calculations and for studying. Reports of many of the investigations
will be turned in for a grade. Unless otherwise specified, you will complete forms for data, answer
questions and do calculation specified in the lab manual, rather than turning in a full lab report. Please
type onto the forms or word-process new tables onto another sheet of paper. Reports are due at the
beginning of lab.
Exam I 100 points
Exam II 100 points
Final Exam 200 points
Cognitive Genetics Tutors Participation* 60 points
Report on Investigation 11 25 points
Questions from Alu lab 25 points
Report on Investigation 24 25 points
B. rapa analysis 25 points
Total: 530 points
Participation in the exercises, pretests, posttests, etc.
Dr. Alice G. Lee
Journals Received by W&J Library:
Journal of Heredity
Journal of Biological Chemistry
Books at W&J Library:
Drlica. Understanding DNA & Gene Cloning: A Guide for the Curious.
Tudge. The Engineer in the Garden: Genes and Genetics: From the Idea of Heredity to the
Creation of Life.
Lewin. Genes IV.
Bollag and Edelstein. Protein Methods.
Coombs. Dictionary of Biotechnology. 2nd ed.
Berg. Dealing with Genes.
McGrayne. Nobel Prize Women in Science.
Hoy. Insect Molecular Genetics.
Corcos. Gregor Mendel’s Experiments on Plant Hybrids.
Johnson. How Scientists Think.
Weismann. Democracy and DNA.
Kitcher. The Lives to Come: The Genetic Revolution and Human Possibility.
Vaughan. How Life Begins: The Science of Life in the Womb.
Aldridge. The Thread of Life: The Story of Genes
Books of Dr. Lee’s:
Lewin. Genes VII.
Maniatis. Molecular Cloning.
Watson. Recombinant DNA Technology, 2nd. ed.
Alberts. Molecular Biology of the Cell, 4th ed.
Bolsolver. From Genes to Cells.
Weaver. Molecular Biology, 2nd ed.
Campbell and Heyer. Genomics, Proteomics and Bioinformatics.
Videos of Dr. Lee’s:
Discovering Women (in Science)
Murder, Rape and DNA
Recombinant DNA Technology
The Building Blocks of Life
The Double Life of RNA (HHMI)
The Secret of Life (Biology Department)
The Immune System (HHMI)
Making Babies (PBS)
Clockwork Genes (HHMI)
A Harvest of Fear (PBS-NOVA)
CD-ROMS of Dr. Lee’s:
Explorations in Cell Biology and Genetics
An Electronic Companion to Genetics
Cells and Genes
Genetics Science Learning Center
Experiments and background information
Go to "Basic Genetics" at http://gslc.genetics.utah.edu/
Mendel Web: Experiments in Plant Hybridization
or use Yahoo to search for “MendelWeb” or http://morgan.harvard.edu
BioMolecules in the Classroom: Educational Molecular Visualization with Chime & RasMol
EXTOXNET - The Extension TOXicology NETwork (pl of 3)
Dolan DNA Learning Center of Cold Spring Harbor Laboratory http://dnalc.org
The National Center for Biotechnology Information
BioChemNet. . . a directory of biology and chemistry educational resources
Medscape Home Page
Biology Place (Benjamin-Cummings)
Inheritance Patterns Analysis: Cotrix
The Biology Project (tutorials, animations and “Test Yourself”)
The National Biotechnology Information Facility
(tutorials and information, including ABO Blood Group tutorial)
The Darwin Awards (for your amusement)