Your SlideShare is downloading. ×
AGRI 305: Agricultural Genetics and Biotechnology Syllabus



AGRI 305
Agricultural Genetics Syllabus – Spring 2009
Instru...
AGRI 305: Agricultural Genetics and Biotechnology Syllabus




           Student Learner Outcomes & K-12 Content Standard...
AGRI 305: Agricultural Genetics and Biotechnology Syllabus




                                              Specifics:
• ...
AGRI 305: Agricultural Genetics and Biotechnology Syllabus




Week of:                  Lecture:
Jan.26th          Chapte...
AGRI 305: Agricultural Genetics and Biotechnology Syllabus


          Activity: Quiz 6: Problem set from Chapter 9
      ...
AGRI 305: Agricultural Genetics and Biotechnology Syllabus


        Homework Questions from Chapter 22: 1-15

Apr.27th   ...
AGRI 305: Agricultural Genetics and Biotechnology Syllabus


Guidelines for lab report: Provide the following categories –...
Upcoming SlideShare
Loading in...5
×

AGRI 305 Agricultural Genetics

870

Published on

0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
870
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
4
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Transcript of "AGRI 305 Agricultural Genetics"

  1. 1. AGRI 305: Agricultural Genetics and Biotechnology Syllabus AGRI 305 Agricultural Genetics Syllabus – Spring 2009 Instructor: Dr. Cynthia A. Daley Email: cdaley@csuchico.edu Class Schedule: MWF 9:00 – 10:00 Lecture: Plumas 205 Activities: Sec 01: Monday 2:00 - 3:50: Plumas 315 Sec 02: Monday 5:00 – 6:50: Plumas 315 Sec 03: Wednesday 2:00 – 3:50: Plumas 315 Office Hours: M/W 10:00 am – 1:00 pm Plumas Hall 209 Ext. 6280 (or by appointment) Required Text: Genetics: Conceptual Approach : 2nd edition Author: Benjamin A. Pierce : ISBN 0-7167-7549-2 + Solutions Manual CD Rom ISBN 0-7167-6835-6 Student On-line help: www.whfreeman.com/pierce2e Course Content: Agricultural Genetics will introduce students to a broad range of topics in the exciting field of population and molecular genetics. Upon completion of this course, the student will be fluent in basics of Mendelian, Molecular, and Population Genetics. In addition, students will become familiar with the application of basic theory to both Plant and Animal Biotechnology. These important concepts will provide insight for all types of students involved in Agricultural Science, Biology and Environmental Science. Laboratory activities will focus on the applications of genetics and biotechnology to agriculture. Students will learn basic aseptic technique, solutions calculations and micro-pipetting skills as an introduction before moving onto basic biotechnology techniques. Students will learn to extract DNA from tissue and process samples for subsequent analysis using Polymerase Chain Reaction (PCR). as a method to detect a single gene in plant tissue to verify seed contamination or GMO status. In addition, students will learn how to transform cells with plasmid vectors to propagate specific gene sequences and produce mass quantities of bio-engineered proteins. These more advanced techniques are used commercially for the purposes of quality control, pathogen detection, specie verification and gene propagation. Both plant and animal application will be included to broaden the scope of the course. Course grading: 13 quizzes (25 pts each) 325 pts Pop Quizzes (10 pts each) 100 pts Lab Reports (50 points each) 150 pts Final 150 pts Total: 725 pts Grade Scale: 90% = A; 80% = B; 70% = C; 60% = D <60 = F 1
  2. 2. AGRI 305: Agricultural Genetics and Biotechnology Syllabus Student Learner Outcomes & K-12 Content Standards Material covered in AGRI 305 covers all of the new Content Standards for California K-12 Public Schools in Biological Sciences, specifically Biology/Life Sciences, Section Genetics, No. 2.a. through 2.g.; 3.a. through 3.d.; 4.a. through 4.f.; 5.a. through 5.e. See the following website for the current Content Standards in it’s entirety. http://www.cde.ca.gov/cdepress/standards-pdfs/science.pdf Material covered in AGRI 305 addresses the following SLO’s for the College of Agriculture degree programs in Agriculture Science and Animal Science: • Technical competency in plant and animal genetics • Demonstrate the application of technology to Agriculture • Demonstrate the ability to identify the appropriate methodologies to solve analytical problems Ground Rules Quizzes will be given at the beginning of each lab session and will cover the previous week’s lecture and laboratory information. Punctuality is important to insure that each student has the maximum amount of time to complete the quiz. Makeup-quizzes will be at the discretion of the Professor, and under most circumstances will not be granted. If there is evidence that you have been involved in any form of academic dishonesty, you will receive an “F” grade for the course, and a report will be provided to Student Judicial Affairs for further action. If a student feels an error in grading has been made, the student has one week from the time of the assignment is returned to them (or the grade is posted on the web, whichever is later) to request a review of the grade. The request must be in writing – attached to the original assignment—and must include a specific statement as to what is in error, how it should be corrected, and what supporting evidence is available. Expected Student Behavior in the Classroom • Students are expected to turn off all pagers, cell phones and other electronic devices during class time. • Students are expected to pay attention and participate in class meetings. • Students may not read other materials (newspapers, magazines) during class. • Students are to remain in class during the entire session with the exception of breaks. Students are not allowed to come and go during the class session. • All class participants are expected to exhibit respectful behavior • All students have the right and privilege to learn in the class, free from harassment and disruption. • Inappropriate or disruptive behavior will not be tolerated, nor will lewd or foul language. • The class follows the standards set in the Code of Students Rights and Responsibilities (EM 96-38) and students are subject to disciplinary action for violation of that code. 2
  3. 3. AGRI 305: Agricultural Genetics and Biotechnology Syllabus Specifics: • There will be no makeup quizzes unless prior arrangements have been made with the Professor two weeks in advance of the scheduled absence. • Weekly quizzes provide the necessary immediate feedback to assess your performance in the class. If you are not meeting your expectations, then change your strategy. It is the responsibility of the student to find the necessary help. Opportunities for help include active participation in a study group, optional tutoring and attending office hours on a regular basis will enhance class performance. • Homework assignments are listed on the syllabus are required and will be covered on the weekly quizzes. • Laboratory reports are required for each of 3 exercises. Each exercise will be written up using standard laboratory format – see laboratory formatting information found on page 7 of this syllabus. • Attendance is absolutely necessary and is required. • Attendance is absolutely necessary and is required. • Attendance is absolutely necessary and is required. • Plan to study more for this class than most of your other classes unless you have an exceptionally strong background in biology and chemistry. Begin by reading the assignment prior (this means before) to coming to class. The material will make more sense if you have seen it once before. Some students will need to re-read the material, then do the homework problems. • Efficient students will require at least 8 -12 hrs of study/week (8 -12 hours in addition to the time spent in class). • Use office hours. • See WebCT for study tips: there are number of good resources available to help you. http://studytips.aac.ohiou.edu/ 3
  4. 4. AGRI 305: Agricultural Genetics and Biotechnology Syllabus Week of: Lecture: Jan.26th Chapter 1: Introduction to Genetics: Reading assignment: page 1 – 13. Activity: Assessment Activity & Assigning Study Groups and Tutoring Homework: Chapter 1: Questions 1 - 26 . Feb 2nd Chapter 2: Reading assignment: page 17 – 44. Chromosomes and Cellular Reproduction: Basic cell types (prokaryotic and eukaryotic); cellular reproduction; sexual reproduction and genetic variation; meiosis and mitosis Activity: Quiz 1: Mitosis and Meiosis Activity: Problems sets Homework: Chapter 2: Worked Problems and Questions 1-30 Feb.9th Chapter 3: Reading assignment: page 47 – 71. Basic Principles of Heredity: Mendelian laws; monohybrid crosses; multiple-loci crosses; Goodness-of-Fit chi square test; penetrance and expressivity Activity: Quiz 2: Problem set from Chapter 3 Homework: Chapter 3 Questions 1 – 29, 35, 37. Feb.16th Chapter 4: Reading assignment: page 75 – 97. Sex Determination and Sex-Linked Characteristics: Sex determination; sex-linked characteristics Activity: Quiz 3 Problem set from Chapter 4 Homework: Chapter 4 Questions 1 –37. Feb. 23rd Chapter 5: Reading assignment: page 101 - 128. Extensions and Modifications of Basic Principles: Lethal alleles; multiple alleles; gene interaction; sex-influenced and sex-limited characteristics; interaction between genes and environment Activity: Quiz 4: Problem set from Chapter 5 Homework: Chapter 5 Questions 1 – 24. March 2nd Chapter 5: Reading assignment: page 101 - 128. Extensions and Modifications of Basic Principles: cont’d. Activity: Quiz 5: Problem set from Chapter 5 Homework: Chapter 5 Questions 1 – 39. Mar.9th Chapter 9: Reading assignment: page 235 – 262. Chromosome Variation: Chromosome morphology; chromosome rearrangement including duplications, deletions, inversion and translocations; aneuploidy; polyploidy; chromosome mutations and cancer 4
  5. 5. AGRI 305: Agricultural Genetics and Biotechnology Syllabus Activity: Quiz 6: Problem set from Chapter 9 Homework: Questions 1 – 27, 33, 34, 35, 36, 38. Mar.16th Spring Break. Enjoy yourself. Mar.23rd Chapter 10: Reading assignment: page 266-283. Chapter 12: Reading assignment: page 318-343. DNA: The Chemical Nature of the Gene: molecular basis of heredity; structure of DNA; special structures of DNA and RNA. DNA Replication and Recombination: Semi- conservative replication; mechanism of replication. Activity: Quiz 7: Aseptic tissue culture – propagating plants from plant tissue. Discussion on how tissue culture is used to incorporate new genes into plant tissue to create a transgenic plant. Homework Questions from Chapter 10: 1-17 & Chapter 12: 1-15. Mar.30th Chapter 13: Reading assignment: page 348 – 371. Chapter 15: Reading assignment: page 409 – 428. Gene architecture; Transcription; Translation Activity: Quiz 8: Introduction to Polymerase Chain Reaction. How PCR has revolutionized society. DNA extraction and preparing samples for PCR. The class with use PCR to identify GMO’s in food products and feeds. Homework Questions from Chapter 13: 1-16 & Chapter 15: 1-16. Apr.6th Chapter 18: Reading assignment: page 509 – 538. Recombinant DNA Technology. Basics concepts of recombinant DNA technology; techniques; applications of recombinant DNA technology Activity: Quiz 9: Gel Electrophoresis and Data Interpretation. Homework Questions from Chapter 18: 1-28. Apr.13th Chapter 18: Reading assignment: page 538 – 549. Recombinant DNA Technology. Applications of Recombinant DNA Technology. Activity: Quiz 10: Cellular Transformation: Part I: Introduction of a new or novel gene into bacteria. Heat Shock Cell Transformation Technique. Operon discussion. Homework Questions from Chapter 18: 29-41. Apr.20th Chapter 22: Reading assignment: page 642 – 658. Quantitative Genetics: relation between genotype and phenotype; statistical methods for analyzing quantitative characteristics; heritability; Activity: Quiz 11: Cellular Transformation: Part II: Differential plating techniques to demonstrate inducible operons and gene expression 5
  6. 6. AGRI 305: Agricultural Genetics and Biotechnology Syllabus Homework Questions from Chapter 22: 1-15 Apr.27th Chapter 22: Reading assignment: page 658 – 671. Quantitative Genetics: heritability; response to selection Activity: Quiz 12: Cellular Transformation: Part III: Protein extraction and Quantification (a.k.a. here is where we find out how much protein was expressed in our transgenic bacteria) Homework Questions from Chapter 22: 15-41. May 4th Chapter 23: Reading assignment: page 676 – 697. Population and Evolutionary Genetics: genetic variation; Hardy-Weinberg Law; nonrandom mating; changes in allelic frequencies; genetic drift; natural selection Activity: Quiz 13: Observe tissue culture results. Discuss how differential media or PCR could be used to determine if a newly propagated plant contains a specific gene, like Bt. Homework Questions from Chapter 23: 1-23 May 11th Chapter 23: Reading assignment: page 697– 711. Population and Evolutionary Genetics: Molecular Evolution Activity: Quiz 14 (optional): Problem Solving Session. Homework Questions from Chapter 23: 24-43. May18th Finals Week : Study your quizzes for the final. 6
  7. 7. AGRI 305: Agricultural Genetics and Biotechnology Syllabus Guidelines for lab report: Provide the following categories – clearly labeled, professionally presented, 12 pt font with 1” margins. (5 pts for presentation) Title: Descriptive terms of the exercise. Date/Author/other pertinent information Introduction: (10 pts) • Statement of goal and objectives (activities to achieve goal) • Provide background information – explain the concept • Discuss any previous work in the area with relation to agriculture – discuss other applications – provide references Materials and Methods (10 pts) • Provide overview of activities and accomplishments • The “what and how” of the activity Results (10 pts) • Provide clear representation of results • No discussion – just presentation of the results Discussion (10 pts) • Discuss your results at it relates to the goals and objectives above • How does your work compare the rest of the class – explain • What could you have done differently to improve your result How does this exercise relate to production agriculture – cite specific examples of how this could be used or is used in the industry. Provide all references. Conclusions (5 pts) • Summarize the work • Summarize the outcomes • Rate the lab for learning objectives – did learn anything? References (5 pts) • List all sources of information in APA format Extra Credit: The following suggestions are provided for those students interested in extra-credit points. 1. Serve as a judge for the AGRI Science Fair on March 14th, 2009 – service is required between 7:00 am and 12:00 noon. (20 bonus pts). Signup list will be circulated in class. If you sign up for this opportunity you must fulfill your obligation. 2. Prepare a full lesson plan for genetics suitable for High School course in Agri-Science or Biology. (20 bonus pts). The EC must include objectives, student learner outcomes, course material to cover, and describe in detail the student activity you would do to engage students with hands on learning. 3. Write a review paper on a how biotechnology is currently being applied to improve air, water and soil quality. 7

×