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  • Red and green=inferred, Blue=mapped
  • Growth reduction when applied in actively growing stages Oxoglutaric acid is the co-substrate of the dioxygenases. TE competes with 2-oxoglutarate and blocks the 3B-hydroxylation (biological activation of GAs) Also thought to block 2B hydroxylation
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    1. 1. Barley, Beer, and Associations with Wolves CIHEAM International Masters in Plant Breeding May 4, 2009 Lecture 1
    2. 2. Barley
    3. 3. Reinheitsgebot 1516 Beer
    4. 4. Association (mapping)
    5. 5. Dr. Bob Wolfe
    6. 6. Wolfe R.I. and J.D. Franckowiak. 1991. Multiple dominant and recessive genetic marker stocks in spring barley. Barley Genetics Newsletter. 20:117–121
    7. 7. Doubled Haploid Production Hordeum vulgare 2N = 2X = 14 Hordeum bulbosum 2N = 2X = 14 X
    8. 8. Genotype: Vv Wst wst Interspecific Cross
    9. 9. 1. Fertilization triggers embryo development. 2. Spontaneous elimination of H. bulbosum chromosomes.
    10. 10. 3. Development of haploid H. vulgare embryos representing a random sample of gametes. N = 7 4. Rescue and culture of haploid embryos.
    11. 11. 5. Double chromosome complement with colchicine. 2N = 2x = 14
    12. 12. 6. Progeny are a set of homozygous genotypes representing a random sample of F2 gametes.
    13. 13. OWB-D X OWB-R F1 94 doubled haploids
    14. 14. <ul><li>An immortal reference population </li></ul><ul><li>Share homozygous seed </li></ul><ul><li>Continually add data </li></ul><ul><li>Build and share research and instruction tools </li></ul>http://barleyworld.org/oregonwolfe.php
    15. 15. Oregon Wolfe Barley Map Centromere Morphological marker
    16. 17. Chutimanitsakun et al. In preparation. 2383 loci + RAD markers (Floragenex)
    17. 25. Ppd-H2 blp Ppd-H1 wst VRN-H1 VRN-H2 MFT VRN-H3 1H 2H 3H 4H 5H 6H 7H
    18. 26. P. Szűcs, V. Blake, P.R. Bhat, S. Chao, T.J. Close, A. Cuesta-Marcos, G.J. Muehlbauer, L. Ramsay, R. Waugh, and P. M. Hayes 2009. An integrated resource for barley linkage map and malting quality QTL alignment. The Plant Genome. In press.
    19. 27. 2-row vs. 6-row
    20. 28. r 2 Genetic Distance (cM) – Chromosome 2H Extent of LD in the CAP Core (all)
    21. 29. Accounting for structure Markers -log P Markers -log P Not accounting for structure
    22. 30. Winter vs. Spring
    23. 31. <ul><li>Winter cereals </li></ul><ul><li>Better water use efficiency </li></ul><ul><li>Higher yield </li></ul><ul><li>Rotational advantages </li></ul>Winter vs. spring
    24. 32. Hulled vs. Hull-less
    25. 33. Food Barley <ul><li>Health benefits </li></ul><ul><ul><li>High fiber </li></ul></ul><ul><ul><li>LDL cholesterol reduction </li></ul></ul><ul><ul><li>Type II diabetes management </li></ul></ul><ul><ul><li>Reduce Obesity </li></ul></ul><ul><ul><li>Increase Satiety </li></ul></ul>
    26. 35. Cistue et al. In preparation 100+ new OWBs + RAD map

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