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  • Red and green=inferred, Blue=mapped
  • Growth reduction when applied in actively growing stages Oxoglutaric acid is the co-substrate of the dioxygenases. TE competes with 2-oxoglutarate and blocks the 3B-hydroxylation (biological activation of GAs) Also thought to block 2B hydroxylation

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  • Barley, Beer, and Associations with Wolves CIHEAM International Masters in Plant Breeding May 4, 2009 Lecture 1
  • Barley
  • Reinheitsgebot 1516 Beer
  • Association (mapping)
  • Dr. Bob Wolfe
  • Wolfe R.I. and J.D. Franckowiak. 1991. Multiple dominant and recessive genetic marker stocks in spring barley. Barley Genetics Newsletter. 20:117–121
  • Doubled Haploid Production Hordeum vulgare 2N = 2X = 14 Hordeum bulbosum 2N = 2X = 14 X
  • Genotype: Vv Wst wst Interspecific Cross
  • 1. Fertilization triggers embryo development. 2. Spontaneous elimination of H. bulbosum chromosomes.
  • 3. Development of haploid H. vulgare embryos representing a random sample of gametes. N = 7 4. Rescue and culture of haploid embryos.
  • 5. Double chromosome complement with colchicine. 2N = 2x = 14
  • 6. Progeny are a set of homozygous genotypes representing a random sample of F2 gametes.
  • OWB-D X OWB-R F1 94 doubled haploids
    • An immortal reference population
    • Share homozygous seed
    • Continually add data
    • Build and share research and instruction tools
    http://barleyworld.org/oregonwolfe.php
  • Oregon Wolfe Barley Map Centromere Morphological marker
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  • Chutimanitsakun et al. In preparation. 2383 loci + RAD markers (Floragenex)
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  • Ppd-H2 blp Ppd-H1 wst VRN-H1 VRN-H2 MFT VRN-H3 1H 2H 3H 4H 5H 6H 7H
  • P. Szűcs, V. Blake, P.R. Bhat, S. Chao, T.J. Close, A. Cuesta-Marcos, G.J. Muehlbauer, L. Ramsay, R. Waugh, and P. M. Hayes 2009. An integrated resource for barley linkage map and malting quality QTL alignment. The Plant Genome. In press.
  • 2-row vs. 6-row
  • r 2 Genetic Distance (cM) – Chromosome 2H Extent of LD in the CAP Core (all)
  • Accounting for structure Markers -log P Markers -log P Not accounting for structure
  • Winter vs. Spring
    • Winter cereals
    • Better water use efficiency
    • Higher yield
    • Rotational advantages
    Winter vs. spring
  • Hulled vs. Hull-less
  • Food Barley
    • Health benefits
      • High fiber
      • LDL cholesterol reduction
      • Type II diabetes management
      • Reduce Obesity
      • Increase Satiety
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  • Cistue et al. In preparation 100+ new OWBs + RAD map