The next generation of
Gene Delivery

Mohit B. Patel
What is Nano?
Macro

Nano

Millionth	
  (10-­‐6)	
  	
  

Human	
  Being	
  
6	
  feet	
  tall	
  

Micro

Billionth	
  (1...
Nanotechnology
Energy

Nanomedicine

Nanosensors

Broad	
  range	
  of	
  applica<ons	
  
Genetic Material

Tissue	
  

Gene	
  

Cell	
  

Body	
  
Chromosome	
  

DNA	
  
Genetic Disorders
Illness	
  caused	
  by	
  an	
  abnormality	
  in	
  an	
  individuals	
  
DNA/gene.	
  
Gene Delivery

Gene
Delivery

Gene
Expression

Research applications: Genetic disorders, gene therapy, protein
production,...
Gene Delivery

Transfection: Laboratory procedure to delivery foreign DNA
into host cells.
Physical methods
Electropora5on	
  

Gene	
  gun	
  

Microinjec5on	
  

Advantage:	
  Can	
  transfer	
  large	
  DNA	
  ...
Biological methods

Advantage:	
  Stable	
  transfec<on	
  
Disadvantage:	
  High	
  risk	
  and	
  <me	
  consuming	
  
Chemical methods
Calcium	
  phosphate	
  

Lipids	
  

Nanotubes	
  

Advantage:	
  Good	
  efficiency	
  
Disadvantage:	
  ...
Limitations of Existing Products
•  Toxic to cells

$6000 and
30 days / year for
each scientist

•  Limited size
(kilobase...
Solution: Janus Transfection Kit™
Janus Transfection Kit™ utilizes a new class of chemical
reagent for DNA transformation ...
Janus Transfection
Reagent™

STEP 1
STEP 2
STEP 3
Efficiency: Janus Transfection Kit™
60
50
40
30
20
10
0

Yeast
Colony forming units

Colony forming units

Bacteria

Janus...
Competitive Advantages
Janus Transfection Kit™ will save each researcher
an estimated 65% of cost and 50% of time.
Company...
Solution: Janus Transfection Kit™
Enables use of larger DNA
High efficiency
Non-mutagenic and non-cytotoxic

Broad range o...
Transfection Market
$13.4 Billion market
DNA and RNA delivery
$346 Million
Genetix Fusion accessible market
Academia 68% (...
Critical Milestones
Launch first
product

Genetix Fusion
founded
Alpha
prototype
tested

Initial funding
raised

May
2012
...
Now Seeking
1.  Academic researchers for beta
testing
2.  Collaborative opportunities
3.  Distribution partner
4.  Seed st...
REDEFINING TRANSFECTION
Contact us:
mohitpatel87@gmail.com
www.genetixfusion.com
(314) 516-4607
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Mohit Patel -- Disruptive Diner: Nano Possibilities

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Presentation from 10/15/13 on innovative startup producing cost effective nano particle kits to get large amounts of DNA into cells without damaging them. Genetix Fusion LLC founder Mohit Patel explains how nanotech makes this possible and the enormous time and cost problems they're able to address. Join OpenlyDisruptive.org for live access to more disruptive innovation events.

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Mohit Patel -- Disruptive Diner: Nano Possibilities

  1. 1. The next generation of Gene Delivery Mohit B. Patel
  2. 2. What is Nano? Macro Nano Millionth  (10-­‐6)     Human  Being   6  feet  tall   Micro Billionth  (10-­‐9)     Red  Blood  Cell   8  micrometer   DNA   2nm  wide  
  3. 3. Nanotechnology Energy Nanomedicine Nanosensors Broad  range  of  applica<ons  
  4. 4. Genetic Material Tissue   Gene   Cell   Body   Chromosome   DNA  
  5. 5. Genetic Disorders Illness  caused  by  an  abnormality  in  an  individuals   DNA/gene.  
  6. 6. Gene Delivery Gene Delivery Gene Expression Research applications: Genetic disorders, gene therapy, protein production, functional genomics, disease diagnostics and agriculture crop enhancement
  7. 7. Gene Delivery Transfection: Laboratory procedure to delivery foreign DNA into host cells.
  8. 8. Physical methods Electropora5on   Gene  gun   Microinjec5on   Advantage:  Can  transfer  large  DNA  molecules   Disadvantage:  Highly  cytotoxic  and  expensive  
  9. 9. Biological methods Advantage:  Stable  transfec<on   Disadvantage:  High  risk  and  <me  consuming  
  10. 10. Chemical methods Calcium  phosphate   Lipids   Nanotubes   Advantage:  Good  efficiency   Disadvantage:  Cytotoxic  and  limited  gene<c  informa<on  
  11. 11. Limitations of Existing Products •  Toxic to cells $6000 and 30 days / year for each scientist •  Limited size (kilobases, kb) •  Poor efficiency •  Time consuming •  Inconsistent results •  Costly 11   “Transfection market clamors for silver bullet.”
  12. 12. Solution: Janus Transfection Kit™ Janus Transfection Kit™ utilizes a new class of chemical reagent for DNA transformation and transfection. New Class = non-lipid, non-viral and non-dendrimer based transfection reagent Janus Transfection Kit™ Proprietary Platform Technology 12  
  13. 13. Janus Transfection Reagent™ STEP 1 STEP 2 STEP 3
  14. 14. Efficiency: Janus Transfection Kit™ 60 50 40 30 20 10 0 Yeast Colony forming units Colony forming units Bacteria Janus Transfection Promega's Product Reagent™ 600 500 400 300 200 100 0 Mammalian cells Number of cells transfomed 0.00% Janus Transfection Reagent™ Control Standard Reagent Plants 100.00% 50.00% Janus Transfection Reagent™ 60 50 40 30 20 10 0 Janus Transfection Reagent™ Control
  15. 15. Competitive Advantages Janus Transfection Kit™ will save each researcher an estimated 65% of cost and 50% of time. Company In-House Invitrogen Sigma Aldrich Promega Qiagen DNA Size <7kb ~9-12kb ~11kb ~7-10kb ~9-12kb Toxicity High High Low Low Medium Genetix Fusion >20kb None Very High 15   Cell Versatility Low High Medium Medium Medium Price $ 413 $ 485 $ 333 $ 440 $ 288 $ 350
  16. 16. Solution: Janus Transfection Kit™ Enables use of larger DNA High efficiency Non-mutagenic and non-cytotoxic Broad range of applications
  17. 17. Transfection Market $13.4 Billion market DNA and RNA delivery $346 Million Genetix Fusion accessible market Academia 68% ($235 M) Pre-packaged kits 75% ($259 M)
  18. 18. Critical Milestones Launch first product Genetix Fusion founded Alpha prototype tested Initial funding raised May 2012 PCT application Provisional patent Beta prototype 1% Market (~5500 units) Controlled market release January 2018 January 2014 Establish operations Today January 2015 Strategic distribution partner 4% Market (~28000 units)
  19. 19. Now Seeking 1.  Academic researchers for beta testing 2.  Collaborative opportunities 3.  Distribution partner 4.  Seed stage investment
  20. 20. REDEFINING TRANSFECTION Contact us: mohitpatel87@gmail.com www.genetixfusion.com (314) 516-4607

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