Tomato-derived CompoundsImprove Abnormalities inHepatic Lipid Metabolism.
We Love Fat.
Fatty Liver…
There Are Many PeopleHaving Fatty Liver.
One of Three Japanese HasFatty Liver.
Fatty Liver PatientsHave Increased.
HealthyLiver
HealthyLiverFatty Liver(NAFLD)
HealthyLiverFatty Liver(NAFLD)NASH
HealthyLiverFatty Liver(NAFLD)NASHFibrosis
HealthyLiverFatty Liver(NAFLD)NASHFibrosisCancersFatty Liver Finally Progressesto Hepatic Cancers.
HealthyLiverFatty Liver(NAFLD)NASHFibrosisFatty Liver Finally Progressesto Hepatic Cancers.Cancers
Fatty Liver Should BeImproved.
We Love Fat.
Nuclear Receptors Are Activatedby Small HydrophobicCompounds.NuclearReceptorLigandRetinoic Acid Receptor(RAR)Vitamin D Rec...
NuclearReceptorNuclear Receptors Are Activatedby Small HydrophobicCompounds.LigandTarget Gene
Nuclear Receptors Are Activatedby Small HydrophobicCompounds.Target GeneNuclearReceptorLigand
Nuclear Receptors Are Activatedby Small HydrophobicCompounds.Target GeneTargetGeneProductsNuclearReceptorLigand
Nuclear Receptors Are Activatedby Small HydrophobicCompounds.Target GeneFood-derivedCompoundNuclearReceptorTargetGeneProdu...
Peroxisome Proliferator-activatedReceptors (PPARs) Are Involvedin Lipid Metabolism.
Insulin SensitivityInsulin SensitivityEnergy ConsumptionEnergy Consumption ?Energy Consumption ?Energy ConsumptionLipogene...
Peroxisome Proliferator-activatedReceptors (PPARs) Are Involvedin Lipid Metabolism.Energy ConsumptionPPARa
Peroxisome Proliferator-activatedReceptors (PPARs) Are Involvedin Lipid Metabolism.02505007501000cont bezaAOX mRNArelative...
PPARa ActivationEnhancesFatty AcidOxidation in theLiver.
Trials to IdentifyTomato-derivedCompoundsActivatingPPARa
050010001500control RF54 RF55 RF56 RF57 RF58 RF59 GWRelativeLuciferaseActivity(%ofcontrol)RF57**We Identifiedan Active Fra...
050010001500control RF54 RF55 RF56 RF57 RF58 RF59 GWRelativeLuciferaseActivity(%ofcontrol)RF57**C18H30O3 (MW: 294)We Ident...
Oxo-octadecadienoic Acids wereResponsible Compounds ofRF57.OCOOH9-oxo-ODA
Oxo-octadecadienoic Acids wereResponsible Compounds ofRF57.OCOOHOCOOH9-oxo-ODA13-oxo-ODA
9-oxo-ODA DecreasedLipid Accumulationin Mouse PrimaryHepatocytes.0* * *2030TGAccumulation(mg/gprotein)10GW7647 (nM)9-oxo-O...
KK-Ay ObeseDiabetic ModelmiceHigh-Fat-DietContaining 13-oxo-ODA (0.02 &0.05%)4 Weeks
50 μmcontrolThe 13-oxo-ODA FeedingDecreased Hepatic TGAccumulation.
50 μmcontrol50 μm0.02%50 μm0.05%The 13-oxo-ODA FeedingDecreased Hepatic TGAccumulation.
0255075100control 0.02% 0.05%(mg/gLiver)*The 13-oxo-ODA FeedingDecreased Hepatic TGAccumulation.*
050100150200control 0.02% 0.05%(mg/dL)*The 13-oxo-ODA-fed MiceShowed Lowering Plasma TGLevels.*
control 0.02% 0.05%Rectal Temperature WasIncreased by the 13-oxo-ODAFeeding.37.037.538.038.5RectalTemperature(oC)**
HealthyLiver
HealthyLiverFatty Liver(NAFLD)High-Fat-Diet
Tomato-derived CompoundsPrevent Fatty LiverDevelopment.HealthyLiverFatty Liver(NAFLD)High-Fat-Diet9/13-oxo-ODAs
Tomato ContainsVarious BioactiveCompounds.
More Tomatoes ...
More Tomatoes ...
More Tomatoes ...Thanks !!!E-mail: nobu@kais.kyoto-u.ac.jp
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Jsolシンポジウム(高橋@京大農学)改定

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These slides were used in "International Symposium on Japanese Solanaceae Genomics Initiative 2013 (JSOL Symposium Tsukuba 2013)".

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Jsolシンポジウム(高橋@京大農学)改定

  1. 1. Tomato-derived CompoundsImprove Abnormalities inHepatic Lipid Metabolism.
  2. 2. We Love Fat.
  3. 3. Fatty Liver…
  4. 4. There Are Many PeopleHaving Fatty Liver.
  5. 5. One of Three Japanese HasFatty Liver.
  6. 6. Fatty Liver PatientsHave Increased.
  7. 7. HealthyLiver
  8. 8. HealthyLiverFatty Liver(NAFLD)
  9. 9. HealthyLiverFatty Liver(NAFLD)NASH
  10. 10. HealthyLiverFatty Liver(NAFLD)NASHFibrosis
  11. 11. HealthyLiverFatty Liver(NAFLD)NASHFibrosisCancersFatty Liver Finally Progressesto Hepatic Cancers.
  12. 12. HealthyLiverFatty Liver(NAFLD)NASHFibrosisFatty Liver Finally Progressesto Hepatic Cancers.Cancers
  13. 13. Fatty Liver Should BeImproved.
  14. 14. We Love Fat.
  15. 15. Nuclear Receptors Are Activatedby Small HydrophobicCompounds.NuclearReceptorLigandRetinoic Acid Receptor(RAR)Vitamin D Receptor (VDR)Estrogen Receptor (ER)etc.
  16. 16. NuclearReceptorNuclear Receptors Are Activatedby Small HydrophobicCompounds.LigandTarget Gene
  17. 17. Nuclear Receptors Are Activatedby Small HydrophobicCompounds.Target GeneNuclearReceptorLigand
  18. 18. Nuclear Receptors Are Activatedby Small HydrophobicCompounds.Target GeneTargetGeneProductsNuclearReceptorLigand
  19. 19. Nuclear Receptors Are Activatedby Small HydrophobicCompounds.Target GeneFood-derivedCompoundNuclearReceptorTargetGeneProducts
  20. 20. Peroxisome Proliferator-activatedReceptors (PPARs) Are Involvedin Lipid Metabolism.
  21. 21. Insulin SensitivityInsulin SensitivityEnergy ConsumptionEnergy Consumption ?Energy Consumption ?Energy ConsumptionLipogenesis ?PPARgPPARdPPARaPeroxisome Proliferator-activatedReceptors (PPARs) Are Involvedin Lipid Metabolism.
  22. 22. Peroxisome Proliferator-activatedReceptors (PPARs) Are Involvedin Lipid Metabolism.Energy ConsumptionPPARa
  23. 23. Peroxisome Proliferator-activatedReceptors (PPARs) Are Involvedin Lipid Metabolism.02505007501000cont bezaAOX mRNArelativeofcontrol(%)Energy ConsumptionPPARa01000200030004000cont bezaCPT-1 mRNArelativeofcontrol(%)
  24. 24. PPARa ActivationEnhancesFatty AcidOxidation in theLiver.
  25. 25. Trials to IdentifyTomato-derivedCompoundsActivatingPPARa
  26. 26. 050010001500control RF54 RF55 RF56 RF57 RF58 RF59 GWRelativeLuciferaseActivity(%ofcontrol)RF57**We Identifiedan Active Fraction ActivatingPPARaUsing Tomato EtOH Extract.
  27. 27. 050010001500control RF54 RF55 RF56 RF57 RF58 RF59 GWRelativeLuciferaseActivity(%ofcontrol)RF57**C18H30O3 (MW: 294)We Identifiedan Active Fraction ActivatingPPARaUsing Tomato EtOH Extract.
  28. 28. Oxo-octadecadienoic Acids wereResponsible Compounds ofRF57.OCOOH9-oxo-ODA
  29. 29. Oxo-octadecadienoic Acids wereResponsible Compounds ofRF57.OCOOHOCOOH9-oxo-ODA13-oxo-ODA
  30. 30. 9-oxo-ODA DecreasedLipid Accumulationin Mouse PrimaryHepatocytes.0* * *2030TGAccumulation(mg/gprotein)10GW7647 (nM)9-oxo-ODA (mM)- 10010 20- -- -
  31. 31. KK-Ay ObeseDiabetic ModelmiceHigh-Fat-DietContaining 13-oxo-ODA (0.02 &0.05%)4 Weeks
  32. 32. 50 μmcontrolThe 13-oxo-ODA FeedingDecreased Hepatic TGAccumulation.
  33. 33. 50 μmcontrol50 μm0.02%50 μm0.05%The 13-oxo-ODA FeedingDecreased Hepatic TGAccumulation.
  34. 34. 0255075100control 0.02% 0.05%(mg/gLiver)*The 13-oxo-ODA FeedingDecreased Hepatic TGAccumulation.*
  35. 35. 050100150200control 0.02% 0.05%(mg/dL)*The 13-oxo-ODA-fed MiceShowed Lowering Plasma TGLevels.*
  36. 36. control 0.02% 0.05%Rectal Temperature WasIncreased by the 13-oxo-ODAFeeding.37.037.538.038.5RectalTemperature(oC)**
  37. 37. HealthyLiver
  38. 38. HealthyLiverFatty Liver(NAFLD)High-Fat-Diet
  39. 39. Tomato-derived CompoundsPrevent Fatty LiverDevelopment.HealthyLiverFatty Liver(NAFLD)High-Fat-Diet9/13-oxo-ODAs
  40. 40. Tomato ContainsVarious BioactiveCompounds.
  41. 41. More Tomatoes ...
  42. 42. More Tomatoes ...
  43. 43. More Tomatoes ...Thanks !!!E-mail: nobu@kais.kyoto-u.ac.jp

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