Preparation of Spotting Solutions based on
Nexterion Spot
Former Product Now
available
Preparation of twofold (2x)
solutio...
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Modification to the Nexterion Spot Spotting buffer composition

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Details of additions to standard Nexterion Spot buffer that will allow spot size adjustment

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Modification to the Nexterion Spot Spotting buffer composition

  1. 1. Preparation of Spotting Solutions based on Nexterion Spot Former Product Now available Preparation of twofold (2x) solution Remark Nexterion Spot I (QMT Spotting Solution I) Nexterion Spot use as supplied Nexterion Spot is identical to Nexterion Spot I Nexterion Spot II (QMT Spotting Solution II) no add 100 µl 2% Cetyl- trimethylammoniumbromid to 10 ml Nexterion Spot final concentration of detergent is 0.02% in the 2x solution Nexterion Spot III (QMT Spotting Solution III) no add 20 µl 10% Triton X100 to 10 ml Nexterion Spot final concentration of detergent is 0.02% in the 2x solution Nexterion Spot AM I (QMT Spotting Solution IV) no 6xSSC with 3 M betaine Nexterion Spot AM II (QMT Spotting Solution V) no add 10 ml 6 M betaine to 10 ml Nexterion Spot General remarks: Nexterion Spot is supplied as a twofold (2x) concentrated solution. The former Nexterion Spot II and III and AM II are also 2x concentrated and are prepared directly from the 2x Nexterion Spot. For further modification of Nexterion Spot to adjust spot size, the concentration of detergent may be varied between 0.01 and 0.1% in the 2x solution. Other detergents as sarcosyl, Tween or SDS may be used too (within same concentration range). For Technical Assistance or Ordering Inquiries, please contact: Europe / Asia – Pacific: USA / Canada Microarray Solutions Microarray Solutions SCHOTT Jenaer Glas GmbH SCHOTT North America, Inc. Otto-Schott-Str. 13 555 Taxter Road 07745 Jena Elmsford, NY 10523 Germany USA Phone: +49-3641-681-91969 Phone: 914-831-2251 Fax: +49-3641-681-970 Fax: 914-831-2353 E-mail: coatedsubstrate@schott.com E-mail: coatedsubstrate@us.schott.com © October 2004

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