NEXTERION® Slide P - The slide of choice for Antibody Microarrays
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NEXTERION® Slide P - The slide of choice for Antibody Microarrays

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NEXTERION® Slide P - The slide of choice for Antibody Microarrays NEXTERION® Slide P - The slide of choice for Antibody Microarrays Presentation Transcript

  • SCHOTT NEXTERION ® NEXTERION Slide P - The slide of choice for antibody microarrays
  • NEXTERION ® Slide P Coating Structure and Probe Attachment Principle Proprietary polymer, ensuring very low non-specific binding Glass support NHS-ester C O O N O O Attached to polymer
  • NEXTERION ® Slide P – Spot morphology Excellent spot morphology on NEXTERION Spot PB Data obtained using a contact printer 4µg/ml RaG in PPB 20µg/ml RaG in PPB Marker spots 100µg/ml RaG in PPB 500µg/ml RaG in PPB 1000µg/ml RaG in PPB 1500µg/ml RaG in PPB 1800µg/ml RaG in PPB P rotein P rint B uffer only 500µg/ml RaG in PP 100µg/ml RaG in PP Marker spots Slide P (30 µg/ml labeled Goat IgG)
  • NEXTERION ® Slide P – Binding capacity High binding capacity on Slide P – up to 1mg/ml probe concentration Hyb concentration in µg/ml IgG from Goat serum (probe) (target)
  • NEXTERION ® Slide P – Background Extremely low backgrounds on Slide P shown with various targets H P E AL
  • NEXTERION ® Slide P – Signal to Bkg ratios Superior S/B ratios for slide P
  • NEXTERION ® Slide P – Signal to Bkg ratios Superior S/B ratios for slide P
  • Spot – Diameter with Next spot PB
    • Spot morphology was good on all substrates
    • But only Nexterion NC-W/D and the FAST slide gave acceptable
    • spot sizes for higher concentrations of detergents in the print buffer
  • Summary of Benchmark Testing       NEXTERION® Slide H NEXTERION® Slide P Fast slide NEXTERION® Slide NC-W (N) NEXTERION® Slide NC-D Path slide Bind. capacity and signal int. (0,25) +++ +++ +++ ++++ +++ + Signal/Background ratio (0,25) ++++ ++++ + ++ +++ +++ Probe target linearity (0,2) +++ +++ ++++ ++++ ++++ + Sensitivity (0,15) ++++ ++++ + ++ +++ ++++ Print buffer tolerance (0,15) ++ ++ ++++ ++++ ++++ ++ Storage of printed slides -20°C -20°C RT RT RT ?
  • Referenced KTH Paper Rebecca Rimini, Jochen M. Schwenk, Mårten Sundberg, Ronald Sjöberg, Daniel Klevebring, Marcus Gry, Mathias Uhlén and Peter Nilsson Validation of serum protein profiles by a dual antibody array approach Journal of Proteomics, Volume 73, Issue 2, 1 December 2009, Pages 252-266 doi:10.1016/j.jprot.2009.09.009
  • KTH results – Slide P had a 100 times lower background than Corning Epoxide 1:500 1:2000 1:8000 Biotin-labeled sera Epoxy Corning Slide P Schott
  • KTH Print buffer contained Trehalose & Pluronic F127 data obtained with non contact printer Nano-Plotter 2.0/E 5% 5% APOH APOA4 Trehalose in the plate Trehalose before printing
  • Assay conditions not described
  • Storage at 4°C for 4 months (KTH data)
  • Effect of environmental conditions on NEXTERION Slide P performance
  • Is NHS hydrolysis an issue during long print runs? Acceptable slide performance after 2 days exposure to 30 – 70 % relative humidity prior to printing
  • Conclusion
    • NEXTERION Slide P has the best S/N ratios of all the SCHOTT slide coatings for antibody arrays
    • Very low non specific binding, and probably lowest of any commercially available microarray slide on the market