SlideShare is now on Android. 15 million presentations at your fingertips.  Get the app

×
  • Share
  • Email
  • Embed
  • Like
  • Save
  • Private Content
 

Optics beyond the Abbe Limit

by Innovation Manager at TLB GmbH on Feb 06, 2012

  • 1,190 views

Present State of Super Resolution Far Field Light Microscopy realized in the Christoph Cremer Lab (December 2011) ...

Present State of Super Resolution Far Field Light Microscopy realized in the Christoph Cremer Lab (December 2011)
Optical Resolution OR (resolvable distance): OR ~ 5 nm (~ 1/100 λexc, from localization precision)
Structural Resolution (imaging capability): ● Mean (2D) distance between individual molecules actually detected: ~ 6 nm ( ~ 1/80 λexc) ● Density of individually detected Molecules : ~ 2,8 • 104/μm2 –
Multicolor- Localization Microscopy: ● 2 – 3 different molecules types ● 3D single Molecule Resolution inside cells (best values): 3D Observation Volume about 1*104 times smaller (i.e. 3D resolution 104 times better) than in conventional Light Microscopy (“Abbe-limit”)
Super Resolution Microscopy with viruses (single molecule imaging)

Statistics

Views

Total Views
1,190
Views on SlideShare
1,189
Embed Views
1

Actions

Likes
0
Downloads
4
Comments
0

1 Embed 1

http://paper.li 1

Accessibility

Categories

Upload Details

Uploaded via SlideShare as Adobe PDF

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
Post Comment
Edit your comment

Optics beyond the Abbe Limit Optics beyond the Abbe Limit Presentation Transcript