The role of the Transcription  Factor Engrailed in Neuronal Identity Nelson Almodóvar Arbelo  University of Puerto Rico at...
What is a transcription factor? <ul><li>Transcription factors are DNA binding proteins. </li></ul><ul><li>They control the...
Transcription Factors define the development of cells A   B       Transcription Factor x  Transcription Factor y
Transcription Factors Regulate Aspects in the Neuronal Development   <ul><li>Neuronal Migration </li></ul><ul><li>Axon pat...
Engrailed during embryogenesis <ul><li>Axon pathfinding </li></ul><ul><li>Synaptic conection/Choice of synaptic partner </...
Engrailed expression in the larva CNS <ul><li>Engrailed is present in Interneurons </li></ul><ul><li>Engrailed is absent i...
Hypothesis:  Over-Expression of Engrailed will perturb neuronal development and/or maintenance.
Methods: Modifying gene expression in Drosophila using the Gal4/UAS system
Methods:  Modifying gene expression in Drosophila using the Gal4/UAS system Early Neuroblasts    UAS – Engrailed Post-Mito...
Objectives <ul><li>AIM1-Check the level Of Engrailed in the Motor neuron  using antibodies against Engrailed. </li></ul><u...
Objectives <ul><li>AIM3-Check growth and morphology of the Neuromuscular Junction by using antibodies against synaptic pro...
References <ul><li>Marie-Bordes, Bruno. Personal interview. 18-25 Sept. 2009. </li></ul><ul><li>S. Tweedie, M. Ashburner, ...
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The role of the Transcription Factor Engrailed in Neuronal Identity

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The role of the Transcription Factor Engrailed in Neuronal Identity

  1. 1. The role of the Transcription Factor Engrailed in Neuronal Identity Nelson Almodóvar Arbelo University of Puerto Rico at Cayey RISE Program student Bruno Marie-Bordes P.h.D. Institute of Neurobiology
  2. 2. What is a transcription factor? <ul><li>Transcription factors are DNA binding proteins. </li></ul><ul><li>They control the transcription of a particular set of genes by specifically binding to their promoters. </li></ul>
  3. 3. Transcription Factors define the development of cells A B Transcription Factor x Transcription Factor y
  4. 4. Transcription Factors Regulate Aspects in the Neuronal Development <ul><li>Neuronal Migration </li></ul><ul><li>Axon pathfinding </li></ul><ul><li>Synaptic connection </li></ul><ul><li>The type of neurotransmitters it uses </li></ul>
  5. 5. Engrailed during embryogenesis <ul><li>Axon pathfinding </li></ul><ul><li>Synaptic conection/Choice of synaptic partner </li></ul>
  6. 6. Engrailed expression in the larva CNS <ul><li>Engrailed is present in Interneurons </li></ul><ul><li>Engrailed is absent in Motorneurons </li></ul>
  7. 7. Hypothesis: Over-Expression of Engrailed will perturb neuronal development and/or maintenance.
  8. 8. Methods: Modifying gene expression in Drosophila using the Gal4/UAS system
  9. 9. Methods: Modifying gene expression in Drosophila using the Gal4/UAS system Early Neuroblasts UAS – Engrailed Post-Mitotic Motorneuron UAS – Engrailed
  10. 10. Objectives <ul><li>AIM1-Check the level Of Engrailed in the Motor neuron using antibodies against Engrailed. </li></ul><ul><li>AIM2-Check in the Central Nervous System for abnormal cell death do to Engrailed overexpression by using antibodies against cell death markers. </li></ul>
  11. 11. Objectives <ul><li>AIM3-Check growth and morphology of the Neuromuscular Junction by using antibodies against synaptic proteins. </li></ul>
  12. 12. References <ul><li>Marie-Bordes, Bruno. Personal interview. 18-25 Sept. 2009. </li></ul><ul><li>S. Tweedie, M. Ashburner, K. Falls, P. Leyland, P. McQuilton, S. Marygold, G. Millburn, D. Osumi-Sutherland, A. Schroeder, R. Seal, H. Zhang, and The FlyBase Consortium. FlyBase: enhancing Drosophila Gene Ontology annotations. Nucleic Acids Research (2009) 37: D555-D559; doi:10.1093/nar/gkn788. </li></ul>
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