Laboratory diagnosis of malaria

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Laboratory diagnosis of malaria

  1. 1. Page 1 of 4LABORATORY DIAGNOSIS OF MALARIAPicture Guide1 Preparing blood films1.1 Preparation of a thin and a thick blood film on the same slideClean the finger Puncture the ball of the finger by lancet Collect the bloodMake thin film from small drop Make thick film from large drop Number the slide (lead pencil)1.2 Common faults in preparing blood films:Poorly positioned blood films Too much blood Too little bloodGreasy slides Edge of the spreader slide chipped
  2. 2. Page 2 of 42 Examining blood filmsHow blood elements appear in thick and in thin blood films?3 Examining blood films for malaria Parasites3.1 Parts of a malaria parasite in a red blood cell3.2 Effect of pH on parasite morphology3.3 Stages of the malaria parasite3.3.1 The trophozoite stageN = Neutrophil,E= Eosinophil,M = Monocyte,L = Lymphocyte,P = Platelets
  3. 3. Page 3 of 43.3.2 The schizont stage3.3.3 The gametocyte stage4 Species of malaria parasite(in Giemsa-stain)1. Plasmodium falciparum 2. Plasmodium vivaxThin film Thick film Thin film Thick film
  4. 4. Page 4 of 43. Plasmodium malariae 4. Plasmodium ovaleThin film Thick film Thin film Thick filmCollected By: Nofel Saleem AsswielMedical Student ,Aden UniversitySecond Year 2012/2013E-mail: Nofel166@gmail.com@nasswielfacebook/nasswiel

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