Histology Lab Slides

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Histology Lab Slides

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Histology Lab Slides

  1. 1. Epithelial Tissue• Characteristics:
  2. 2. Simple (= single layer) Cuboidal• Description: Single layer of cube- shaped cells.• Location: small ducts of glands, kidney Cell is as wide as it is high. One central nucleus tubules, surface of ovary.• Function: secretion & absorption
  3. 3. Simple Cuboidal Lumen: Inside space of• Cells closely packed Tubular structure together and touching one another• Has a basal surface Nucleus Apical Surface and apical surface Basal Surface• Avascular• Sits on a basement membrane (difficult to see on slide)
  4. 4. Non- Ciliated Simple Columnar• Description: Single layer of non-ciliated rectangular cells; contain goblet cells and sometimes microvilli.• Location: GI tract, ducts, gallbladd er• Function: secretion and absorption
  5. 5. • Nuclei lie near base of cell Simple Columnar Cells• Goblets secrete mucous at apical surface to lubricate Goblet Cell lining of Nucleus GI, respiratory, reproductiv e and urinary systems.• Microvilli increase surface area for absorption. Goblet Cell
  6. 6. Pseudostratified Ciliated Columnar Epithelium• Appears stratified because nuclei are at varying levels.• Ciliated lines airways of upper respiratory tract.• Mucus from Goblets Goblet Cells traps foreign particles and are swept away by cilia.
  7. 7. Goblet Cells secreting mucus Cilia Nuclei at varying levels
  8. 8. Epithelial and 1 Connective• 1 – pseudostratified columnar epithelial 2• 2- simple cuboidal epithelial 3• 3- hyaline cartilage connective tissue
  9. 9. Collagen Fiber (thicker pink ones) Loose Areolar GROUND SUBSTANCEConnective Tissue FIBER MATRIX Fibroblast CELLS Elastic Fiber
  10. 10. Teased Dense Regular Connective Tissue• Forms tendons and ligaments and aponeuroses.• Matrix looks white and shiny.• Mostly collagen fibers with fibroblasts between rows.• Strong attachment!
  11. 11. Teased Dense Regular CT (magnified) Fibroblasts Fibers are mostly Collagen.
  12. 12. ReticularConnective Tissue Reticular Cells• Network of interlacing reticular fibers & Blue staining reticular cells Reticular fibers• Found in stroma (framework) of liver, spleen, lymph nodes, red bone marrow• Acts as a filter in spleen and lymph nodes.
  13. 13. Reticular Connective Tissue• Remember that connective tissue is vascular. (Avascular epithelial tissue depends on diffusion of nutrients and waste through blood Blood vessel: Single layer of vessels in CT. Epithelial cells forms vessel tube.
  14. 14. Reticular 1 CT 3• 1- Reticular 2 cells• 2- Blood vessel• 3- Reticular fibers
  15. 15. Hyaline Cartilage• Often called gristle• Bluish white smokey chondrocytes looking ground substance• Numerous chondrocytes• Location: ends of long Ground S bones, ribs, nose, trache a, larynx, bronchi• For flexibility and support• Most abundant type of cartilage in body
  16. 16. Elastic Cartilage• Chondrocytes are located within threadlike network of elastic fibers• Location: Epiglottis of larynx, external ear, Eustachian tubes• For support and shape
  17. 17. Elastic vs Hyaline CartilageLacuna(hollow spaceholding chondrocyte) chondrocyte Smoother Appearance Of ground Elastic fibers throughout substance Ground substance
  18. 18. Adipose Tissue• Adipocytes, “signet ring” cells with peripheral nuclei.• For storage of triglycerides, to reduce heat loss, and serve as an energy nucleus reserve.• Location: under skin, around heart & kidneys, yellow “signet ring” marrow, behind adipocyte eyeball
  19. 19. Osseous Tissue (Bone)• Compact bone consists of osteons (haversion systems) that contain: – Lamellae – Lacuna – Osteocytes – Canaliculi – Central (haversion)canals• Spongy bone consists of thin plates called trabeculae filled with red marrow
  20. 20. Lamellae Central(Haversion) Haversion Canal System Lacunae
  21. 21. Lacunae (where osteocytes reside) Canaliculi: small Tunnels where osteocyte processes run for diffusion of nutrients and wastes
  22. 22. Skeletal Muscle Tissue• Striated – Proteins • Actin Striations • Myosin• Multinucleated• Voluntary• Attach to bones• For movement, communicatio n, posture, heat production
  23. 23. Skeletal muscle fiberwith multiple nuclei
  24. 24. Smooth (Visceral) Muscle Tissue• No striations• Spindle-shaped• Single centrally located nucleus• Nucleus is long and cigar shaped• Involuntary• Viscera• Walls of hollow structures – Blood vessels – Airways to lungs – Stomach – Intestines – Gallbladder – Urinary bladder
  25. 25. Cigar shaped nuclei
  26. 26. Smooth Muscle
  27. 27. Cardiac Muscle Tissue• Striated, mono- nucleated, branching• Self excitatory• Intercalated discs Intercalated disc attach fibers together and contain gap junctions (help with nucleus conduction of electrical signals)
  28. 28. Name the tissues and structures you see.
  29. 29. Simple Squamous Epithelial
  30. 30. Stratified Squamous Epithelial These keratinized epithelial cells will Be sloughed off. Apical Layer Basal Layer
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