Tooth Enamel Erosion
Mónica C. del Moral
RISE Summer 2013
Research Proposal
Inquiry
• We like many beverages that in excess are
bad for our oral health.
• Bacteria and metabolism
Background Information
• Caries
• Tooth enamel
• Streptococcus mutans
• surface profilometer
• focus variation
http://www.upc.edu/pct/es/equip/399/perfilo
metro-mecanico.html
http://www.azom.com/materials-videos.aspx?
cat=19
Profilom...
Hypothesis
• According to the conversion of
carbohydrates to lactic acid by the
streptococcus mutans (S.mutans) bacteria,
...
Objectives
• to determine which of the beverages
chosen is the one that causes the most
damage to the tooth enamel
• to es...
Materials
• Teeth
• Saliva
• Beverages
• Well plates
• Micropipettes
• Petri dishes
• pH meter
• Contact-stylus
surface
pr...
Methodology
• Phase 1
– Petri dishes
• Phase 2
– Premeasures
– Dividing and labeling
– Saliva and beverages
– Add teeth
– ...
Bibliography
– Vieira, A.R, Deelev, K.B, Callahan, N.F, and others; Detection of
Streptococcus mutans Genomic DNA in Human...
– The free dictionary; Profilometer; Farlex; 2007, 2012;
http://medical-dictionary.thefreedictionary.com/profilometer
– Br...
Thank you
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Research proposal Tooth Enamel Erosion

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Research proposal Tooth Enamel Erosion

  1. 1. Tooth Enamel Erosion Mónica C. del Moral RISE Summer 2013 Research Proposal
  2. 2. Inquiry • We like many beverages that in excess are bad for our oral health. • Bacteria and metabolism
  3. 3. Background Information • Caries • Tooth enamel • Streptococcus mutans • surface profilometer • focus variation
  4. 4. http://www.upc.edu/pct/es/equip/399/perfilo metro-mecanico.html http://www.azom.com/materials-videos.aspx? cat=19 Profilometers http://expo21xx.com/sens or/19868_st3_microscope/ default.htm Optical 3D microscope Infinite Focus
  5. 5. Hypothesis • According to the conversion of carbohydrates to lactic acid by the streptococcus mutans (S.mutans) bacteria, the beverage with the most sugar content will cause the most damage to the tooth enamel.
  6. 6. Objectives • to determine which of the beverages chosen is the one that causes the most damage to the tooth enamel • to establish if there is a relation between sugar content and pH level in the erosion of the tooth • To understand why orthodontic treatments forbid the consumption of some beverages.
  7. 7. Materials • Teeth • Saliva • Beverages • Well plates • Micropipettes • Petri dishes • pH meter • Contact-stylus surface profilometer • Optical 3D microscope Infinite Focus
  8. 8. Methodology • Phase 1 – Petri dishes • Phase 2 – Premeasures – Dividing and labeling – Saliva and beverages – Add teeth – Observe – Measurements – Comparison and analysis
  9. 9. Bibliography – Vieira, A.R, Deelev, K.B, Callahan, N.F, and others; Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood; ISRN Dent.; Published online 2011 April 10; http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128887/ – What lives inside your mouth?; http://science.education.nih.gov/supplements/nih2/oral-health/guide/pdfs – What acid is produced by bacteria?; Yahoo answers; 2010; http://in.answers.yahoo.com/question/index?qid=20100622085725AAFMIL0 – Odontología UNA; Streptococcus mutans; http://es.slideshare.net/dicefalo18/streptococcus-mutans – The free dictionary; Dental biofilm; Farlex; 2008; http://medical-dictionary.thefreedictionary.com/dental+biofilm – Fonseca, G.; Esmalte Dental; UNIVERSIDAD PRIVADA ANTERNOR ORREGO; http://www.scribd.com/doc/517866/ESMALTE-DENTAL – The free dictionary; Caries; Farlex; 2007; http://medical-dictionary.thefreedictionary.com/caries
  10. 10. – The free dictionary; Profilometer; Farlex; 2007, 2012; http://medical-dictionary.thefreedictionary.com/profilometer – Brook Anco; Focus Variation Systems; Brook Anco Corporation; 2013; http://www.brookanco.com/Focus_Variation_Systems.html – Vorburger, T.; Optical Methods of Surface Measurements; National Institute of Standards and Technology (NIST); Measurement Science and Standards in Forensic Firearms Analysis 2012 NIST, Gaithersburg, MD; July 10, 2012 http://www.nist.gov/oles/upload/3-Vorburger-Ted- Optical-Methods-of-Surface-Measurement-MSSFAA-10jul12.pdf – Fujii, M.,Kitasako, M.,Sadr,A., Tagami,J.; Roughness and pH changes of enamel surface induced by soft drinks in vitro-applications of stylus profilometry, focus variation 3D scanning microscopy and micro pH sensor; Department of Restorative Sciences, Tokyo Medical and Dental University, Tokyo 113-8549, Japan; 2011; http://lib.bioinfo.pl/pmid:21597209 Bibliography
  11. 11. Thank you

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