New world built with toxic chemical is coming up

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New world built with toxic chemical is coming up

  1. 1. What did we Know???? Carbon, hydrogen, nitrogen, oxygen, phosphorus and sulfur are the six basicbuilding blocks of all known forms of life on Earth.
  2. 2. 1-Phosphorus is part of the chemical backbone of DNA. DNA
  3. 3. 2- Phosphorus is part of thechemical backbone of RNA. RNA
  4. 4. 3- Phosphorus is a central component of the energy-carrying molecule in all cells (adenosine triphosphate) Adenosine triphosphate
  5. 5. 4- Phospholipids that form all cell membranes. phospholipids
  6. 6. 5-NADH & NAD+
  7. 7. What is new????A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus Wolfe-Simon F, Blum JS, Kulp TR, Gordon GW, Hoeft SE, Pett-Ridge J, Stolz JF, Webb SM, Weber PK, Davies PC, Anbar AD, & Oremland RS (2010). NASA Astrobiology Institute, USA.
  8. 8. How ???
  9. 9. IDENTIFICATION CARD OF BACTERIA First Name: GFAJ-1 "Give Felisa a Job". Family Name: Halomonadaceae. Date Of birth: 2 December 2010. Place of bearth: Mono Lake in eastern California, USA. Shape: rod-shaped bacterium. Important Note: The authors used 16S rRNA sequencing to identify this bacterium as a member of the Halomonadaceae family of Gammaproteobacteria.
  10. 10. What is possible?It is theoretically possible that some other elements in the periodic table could serve the same functions. Periodic table
  11. 11. What did they do???They took sediment from Mono Lake in California, a very salty and alkaline lake containing 88 mg of phosphate and 17 mg of arsenic per liter. It also has one of the highest natural concentrations of arsenic in the world. Mono Lake in California
  12. 12. sediment 10 mM glucose 0.8 mM NH4SO4 full vitamins trace mineralsphosphate arsenate basic mediumsimilarly alkaline and hypersaline defined medium at pH 9.8 The original ingredients caused it to contain about 3.1 ( µM phosphate (PO4) and about 0.3 µM arsenate (AsO4).
  13. 13. Basic medium with all ingredients with a regimen of increasing AsO4 (various concentrations) additions initially spanning the range 100 μM to 5 mM The enrichments were taken through many decimal dilution transfers greatly reducing any potential carryover of phosphorus The sixth transfer of the 5 mM AsO4 (no added PO4) condition was closely monitored and demonstrated an approximate growth rate (μ) of 0.1 per day. Put some of the culture onto an agar plate made with the same medium An isolated colony was picked from the agar plates Into an artificial iquid medium with 5 mM arsenate (no added PO4) . They gradually increased the arsenate to 40 mM Members of this family have been shown to accumulate intracellular As.
  14. 14. GFAJ-1 grew at an average μmax of 0.53 day-1 under +As/-P, increasing by over 20-foldin cell numbers after six days. It also grew faster and more extensively with the addition of 1.5 mM PO4 (-As/+P, μmax of 0.86 day-1, Fig. 1A, B). However, when neither AsO4 nor PO4 was added, no growth was observed (Fig. 1A, B). We include both optical density and direct cell counts to unambiguously demonstrate growth using twoindependent methods. Cells grown under +As/-P were oblong and approximately two by one microns when imaged by scanning electron microscopy (Fig 1C, 11). When grown under +As/-P conditions, GFAJ-1 cells had more than 1.5-fold greater intracellular volume (vol. ≈ 2.5 0.4 μm3) as compared to -As/+P (vol. ≈ 1.5 0.5 μm3) (Fig. 1D).
  15. 15. Transmission electron microscopy revealed largevacuole-like regions in +As/-P grown cells that may account for this increase in size (Fig. 1E).
  16. 16. What did They do to determine if GFAJ-1 was taking up AsO4 from the medium Materials and methods
  17. 17. FirstThey measured the intracellular As content by ICP-MS (Inductively coupled plasma mass spectrometry)
  18. 18. The results (% dry weight) Condition (n) As:P As P +As/-P (8) 0.19 ± 0.25 0.019 ± 0.0009 7.3 -As/+P (4) 0.001 ± 0.0005 0.54 ± 0.21 0.002Table 1. Bulk intracellular elemental profile of strain GFAJ1. Cellsgrown and prepared with trace metal clean techniques. Number in parentheses indicates replicate samples analyzed.
  19. 19. SecondThey grew some cells with radioactive arsenate (73-As) and noadded phosphate to obtain more specific information about the intracellular distribution of arsenic They observed intracellular arsenic in protein, metabolite, lipid and nucleic acid cellular fractions Solvent (subcellular fraction) Cellular radiolabel recovered (% of total) Phenol (protein + s.m.w. 80.3 1.7 metabolites) Phenol:Chloroform (proteins + 5.1 4.1 lipids) Chloroform (lipids) 1.5 0.8 Table 2. Intracellular radiolabeled (DNA/RNA) 11.0 distribution, All major cellular Final aqueous fraction 73AsO4 - arsenate 0.1 sub‐fractions contained radiolabel after cell washing procedures. Small molecularweight metabolites (s.m.w. metabolites) potentially include arsenylated analogs of ATP, NADH, acetyl‐CoA and others (11). Standard error shown.
  20. 20. Third To determine if this distribution pattern reflected a use of AsO4 in place of PO4 in DNA, They estimated the average sequenced bacterial genome They used high-resolution secondary ion mass spectrometry (NanoSIMS) to positively identify As in extracted, gel purified genomic DNAThe gel-purified chromosomal DNA from cells grown with arsenate (lane 2) or with phosphate (lane 3),
  21. 21. FourthTo determine the speciation and chemical environment of the intracellular arsenic They used synchrotron X-ray studies The X-ray data support the position of arsenate in a similar configuration to phosphate in a DNA backboneor potentially other biomolecules as well. These data also indicated evidence for the presence of arsenate in small molecular weight metabolites (e.g., arsenylated analogs of NADH, ATP, glucose, acetyl-CoA) as well asarsenylated proteins where arsenate would substitute for phosphate at serine, tyrosine and threonine residues
  22. 22. Type Number R σ2 Table 3. Results of fitting arsenic K- edge EXAFS of GFAJ1, Details forAs-O 4.2 (0.6) 1.73 (2) 0.003 (2) table: S02=1, global amplitude factor and E0= 13.97, offset for calibration. Type, the coordination type;As-C 2.5 (0.5) 2.35 (4) 0.003 (2) Number, the coordination number; R, interatomic distance; σ2, the measure of the static disorder of the shell. See Table S2 for comparison to PAs-C 2.2 (0.5) 2.92 (6) 0.003 (2) in P-containing biomolecules. FIGURE: X-ray analysis of GFAJ-1 +As/-P. (A) EXAFS comparisons of As-S and As-Fe shells to whole GFAJ- 1 cells. The EXAFS fit to GFAJ-1 is shown in red. (B) XRF maps indicating the correlation between As, Fe, and Zn, and not with P. The features with As, Fe and Zn represent small aggregates of cells. The color bar at the bottom shows the relative concentration scale, from low (blue) to high (red).
  23. 23. Conclusion# The data show arsenic-dependent growth by GFAJ-1.# Growth was accompanied by arsenate uptake and assimilation intobiomolecules including nucleic acids, proteins and metabolites.# In some organisms, arsenic induces specific resistance genes to cope withits toxicity.# while some dissimilatory arsenicutilizing microbes can conserve energyfor growth from the oxidation of reduced arsenic species, or ”breathe"AsO4 , as a terminal electron acceptor.# This current study differs because they used arsenic as a selective agentand excluded phosphorus, a major requirement in all hitherto knownorganisms.# GFAJ-1 is not an obligate arsenophile and it grew considerably betterwhen provided with P.# GFAJ-1 can cope with this instability. The vacuole-like regions observedin GFAJ-1 cells when growing under +As/-P conditions are potentiallypoly-β-hydroxybutyrate rich [as shown in other Halomonas species .
  24. 24. References;http://www.nasa.gov/topics/universe/features/ astrobiology_toxic_chemical.htmlhttp://www.sciencemag.org/content/330/6009/ 1302.full.pdfhttp://www.space.com/9631-arsenic-eating- bacteria-opens-possibilities-alien-life.htmlhttp://www.snip.ly/GMUlv

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