2010 年５月３１日 Product Introduction APt. Co., Ltd. Photo: View from Our Compnay, Overlooking Yoyogi Park アプト株式会社
Our Company Aims to Provide Infinite Possibilities of Nanotechnology We want to contribute to the expansion of the use of platinum catalyst worldwide in environmental cleanup, prolonged use of petroleum, post-fossil fuel use and lower carbon emissions. We are substituting the raw materials in dehumidification, sensors, fuel cells, and exhaust filters and reducing the amount of catalysts used industry-wide. We are reducing the amount of platinum used in color panels, molds, materials, et cetera to 1/100th. With our nanotechnology we are providing high quality and efficient material for platinum catalysts and platinum nanocolloids for the use in advanced materials. Develop the understanding of catalyst use as well as sunlight and moisture to cultivate hydrogen energy to be used in solar batteries, fuel cells nano catalyst electrodes, renewable hydrogen catalysts and electrode composite elements. Evidence of the healthy applications of platinum nanotechnology have been found through research and testing. This technology has many applications, including use in drinking water, food, supplements, cosmetics, mouthwash, dentistry materials, medicines and other products. ealth nergy educe echnology
Outline & Background <ul><li>The concept of using platinum started with Doctor Hideyo Noguchi. However over 100 years have passed and the effectiveness of platinum has not been thoroughly developed. On the other hand, industrial uses of platinum have been supported both in the chemical industry and the automotive industry. </li></ul><ul><li>In 2003, Professor Yusei Miyamoto of The University of Tokyo 、 and Professor Naoki Toshima of the Science University of Tokyo, Yamaguchi College were able to convert 2 nanometers of platinum and eradicate the reactive oxygen into an aqueous solution, which had never been accomplished. We are now receiving orders from food manufacturers, cosmetics companies, supplement manufacturers, hair product manufacturers, and dental product manufacturers to take advantage of this.. </li></ul><ul><li>In addition, in an independent 2007 study by Professor Masayuki Yagi of Niigata University, the electrode formation of nano-particles was licensed and industrial use has begun. </li></ul><ul><li>We propose to use original processing technology for the production of next generation platinum materials. Based on basic research, we would like to proliferate high quality platinum nano-colloid for bio-science and industrial applications. </li></ul>Established July 7, ２００６ (registered) September 2, ２００６ (began operations) Head Office Kamiyama-cho Building, 2Fl., 5-2 Kamiyama-cho, Shibuya-ku, Tokyo Capital ２５６ , ３００ . ０００ yen Shares Issued ５１ , ０８０ Representative Director Minenobu Okayama Atsushi Kanazawa Tomoko Omae Masato Sugi Wataru Sato Hidenobu Masuyama （ Outside directors 、 Venture Capital Investors are underlined ） Auditor Takeshi Onogi （ CPA ） Clients Rohto Pharmaceuticals, Kose, HABA Laboratories, Itochu Corporations, Ceres Inc., Marubeni Chemix
Powder Colloidal Platinum Nanoparticles IASO GUM ( content : 150 ｇ） Plati-nanotect （ content 100ml ） Platinum water （ content : 500ml ） Platinum water Apt （ content : 500ml ） cleaning liquid in the oral cavity Platinum content 180~240ppm Platinum Life Platinum Life Product samples ① own brand
cosmetics Mineral water Yogurt Dietary supplement Platinum Life Platinum Life Product samples ② business partner brand
Humans naturally create Super Oxide Dismutase (SOD) and Catalase in large amount in young people. And these anti-oxidant enzymes are effectively removes excess Reactive Oxygen Species (ROS) from their systems. However, levels of SOD and Catalase begin to decline around 30 years of age, and these enzymes are almost gone by the time we reach 35-40. This is thought one of the prime factors behind various aging phenomena and diseases . A g i n g (Age) 30 yrs 10 yrs 40 yrs 50 yrs SOD and Catalase levels Lower(%) 0 ％ Increased levels of ROS Lifestyle Diseases Beginning of Aging
Sources and causes of oxygen radicals are ubiquitous and impossible to avoid. For example, 2% of the oxygen we inhale with every breath is converted to Reactive Oxygen Species (ROS) . Thus, even though sports increases fitness overall, the additional respiration also increases the amount of ROS in the system. Smoking and daily stresses are also abundant sources of ROS. A full 90% of diseases (not including infectious diseases) are caused by cell damage through ROS. Also called oxygen radicals of ROS are particularly involved in lifestyle diseases. Inflammatory Rheumatoid arthritis Atopic dermatitis Gastric ulcers, etc. Circulatory Arteriosclerosis Heart attacks Strokes, etc . Cerebral Alzheimer’s Parkinson’s Retinitis pigmentosa, etc. Cosmetic Blotches Wrinkles Sagging Other age-related issues What are Reactive Oxygen Species (ROS) ? ■ Cells damaged by oxidative stress from ROS are implicated as risk factors in many diseases. Ｓｔｒｅｓｓ Ｏｖｅｒ -eating Cigarettes UV rays Chemicals Air pollution Teeth-whitening Reactive Oxygen Species High reactivity strong oxidizer
APt Colloidal Platinum Nanoparticles ■ The anti-oxidative ability of APt CPNs was measured according to the method described in a published document* and was compared with that of foods in the document. (*: Japan Food Research Laboratories Bulletin No. 32; March 2003) Why platinum? ■ Transition metals such as gold and platinum have the ability to reduce Reactive Oxygen Species (ROS) through their catalytic properties. ■ Out o f all the transition metals, only gold and platinum have been approved for use as food additives in Japan. Platinum also has superior anti-ROS properties as compared with gold. Why nanoparticles? ■ Particle diameter of APt CPNs` Platinum is 2.3 nano-meters (nm) in average. Particles （ < 10 nm in diameter ） account for not less than 90% of nanoparticle distribution. ■ Platinum loses the ability to catalyze reduction when particle diameter is greater than 100 nanometers. ■ APt CPNs are the new mineral ingredient created by Professor Miyamoto, who conducts research on ROS at University of Tokyo Graduate School. It uses a nanocolloidal form of platinum, which is effective in catalyzing reduction of ROS. What are APt Colloidal Platinum Nanoparticles (APt CPNs) ?
ROS types ● Superoxidized anion ● Singlet oxygen ● Hydroxyl radicals ●Hydrogen peroxide ● Hydroxy peroxy radicals ● Hypochlorous acid ● Lipid hydroperoxides ● Peroxyl radicals ● Alkoxyl radicals ● NO ● NO2 ● ( Ozone) APt CPNs are… … active against 4 major ROS found in the human body! 4 major ROS types particularly important for their health effects Anti-ROS substances in food are all active against different types of active oxygen: Superoxidized anion: Vitamin C, bilirubin, etc. Hydroxyl radicals: Vitamin E, beta carotene, flavanoids, linoleic acid, etc. Oxygen singlets: Vitamin C, Vitamin E, Vitamin B1, Beta carotene, etc. Hydrogen peroxide: Vitamin C, catalase, etc. Anti-Oxidative Properties of APt CPNs - 1
Parameters After Consumption of APt CPNs Results Study design Intake Subjects 10 adult males and 15 females who were 20-64 years of age and had hyperlipidemia (total cholesterol: 220 mg/dL, LDL cholesterol: 140 mg/dL, or HDL cholesterol: < 40 mg/dL, or TG: 150 mg/dL) A cross-over single-blind study. Regarding meals, beverages, smoking, and alcohol consumption, patients sustained their daily livings that were not particularly restricted or limited. The 18 g intake group showed significant decreases in blood total cholesterol levels regarding lipid metabolism and in urinary microalbumin excretion regarding renal function, and exhibited a reduction in oxidation stress (d-ROM value). Furthermore, the 36 g intake group also showed significantly enhanced antioxidative ability (BAP value). The sample containing 18 g of APt CPNs or placebo was given by 14-day repeated oral administration to the 18 g intake group and the 36 g intake group.
３． Industry Demand Plastic and synthetic fibers surfaces can also be coated with the material and it has uses in the automotive industry as an exhaust catalyst. It can be used in ink materials and as a silver paste corrosion prevention material. There are also industrial glass applications (computer monitors, televisions, LCD’s, fiberoptics and transparent electrodes.) Organic EL seal water treatment electrolysis solution. Solar battery electrodes 、 especially pigment-sensitized solar arrays. Fuel Cells. Chemical plant reaction catalyst. Powder activation catalyst. Sensor catalysts (CO sensors.) １． Home Appliance Applications There is strong consumer demand for this technology with uses in bread and coffee makers and rice cookers (Z Corporation has already begun use.) ２． Textile Applications There is also demand in the textile industry with makers utilizing the anti-bacterial, -oxidization properties of platinum (KB Corporation has begun use.) Resource Conservation and Efficient Applications of Nanotechnology Expanding Platinum Engineering to New Industries While Contributing to Society Sample Research 03/30/11 Sample Research APt Co., Ltd.
Safety Data of APt CPNs Subjects Results Safety confirmation study in humans 10 males and 15 females aged 20-64 years orally and repeatedly ingested the sample containing 18 g of APt CPNs or placebo, twice daily (1 capsule/ingestion) for 14 days. No adverse events were observed at all throughout the study period. Laboratory tests revealed no deviation from normal values that was attributable to the test food. Therefore, APt CPNs are considered to elicit no safety concerns. Studies on 9 items required for the application of quasi-drugs 90-day repeated oral dose study The concentrate suspension (1 mM) of APt CPNs were used to conduct studies on 9 items that are required for the application of quasi-drugs to the Japanese regulatory authorities. Consequently, no toxicity was found in the acute toxicity study ( > 2,000 mg/kg) and in other study items. APt CPNs were given to rats by 90-day repeated oral administration. Consequently, no changes were observed in general condition, hematology, pathology, and other studies. Furthermore, the estimated no observed adverse effect level is 120 g/kg/day.
Summary <ul><li>Platinum is permitted as food additives in Japanese food safety regulation law. </li></ul><ul><li>Our nanotechnology can make platinum to be liquid or powder type colloidal nano-particles. </li></ul><ul><li>APt Co., LTD. succeeded in maximizing Colloidal Platinum Nanoparticles （ CPN) as a new class of antioxidant and illustrating its potential abilities for metabolic syndromes with animal model tests and human safety test. </li></ul><ul><li>APt Co., LTD. moves ahead on proposal with Colloidal Platinum Nanoparticles. </li></ul><ul><li>Colloidal Platinum Nanoparticles : Patented in Japan, Russia, South Africa, China , applied for USA . </li></ul>