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Mills Cbst
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Bioengineering Red Fluorescent Tags from Thermosynechococcus elongatus ,[object Object],[object Object]
Actual Result
Expected Result
What ’ s the opposite of Eureka? ,[object Object],[object Object],[object Object],[object Object]
Identifying the problem ,[object Object],[object Object],[object Object]
Hypothesizing possibilities ,[object Object],[object Object],[object Object]
Contamination ,[object Object],[object Object],[object Object]
Procedures: preparing DNA for sequencing ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Procedures: Sequencing DNA ,[object Object],[object Object]
Procedures: Interpreting DNA results ,[object Object],[object Object]
Procedures: Interpreting DNA results ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Procedures: Interpreting DNA results ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Procedures: comparing sequences ,[object Object]
Procedures: comparing sequences ,[object Object]
Sequence comparison results ,[object Object],[object Object],[object Object]
Tracking down the source ,[object Object],[object Object]
Moving forward ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Thank you Spiller Lab! ,[object Object]

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Mo's Barret Presentation

Editor's Notes

  1. As Monica mentioned we were getting beautiful blue 911T E. coli pellets.
  2. Color is correct. Other reasons explain the small yield.
  3. Our task was to capture the bacterial population from the blue pellet, grow it in nutrient broth using sterile technique for mini prepping of its plasmid DNA, Finally sequence its plasmid DNA to confirm its identity by aligning it with our known genes.
  4. We are back on track – expressing and characterizing 911 in its truncated GAF only form.
  5. Now Valerie Metea will tell you more about protein purification