Cannabis Testing The Current Landscape

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Cannabis Testing The Current Landscape

  1. 1. Cannabis Testing:The Current Landscape <br />© 2011 Pure Analytics, LLC<br />
  2. 2. Agenda<br />Cannabis Testing: The Current Landscape<br />The importance of testing<br />The dynamic market <br />The issue of oversight<br />Questions patients and collectives should ask<br />Applications of Potency Testing in the Development of non-THC Dominant Strains<br />Identification<br />Predictive crosses and collapsing the cycle time for CBD-rich strain development<br />© 2011 Pure Analytics, LLC<br />
  3. 3. Cannabis Testing: The Current Landscape<br />The Importance of Testing<br />Provide objective information to patients for use in selecting:<br />Cannabinoid profile, THC:CBD<br />Overall dosage level, THC + CBD<br />Promote the availability of medically-important cannabis options such as CBD-rich varieties<br />Through strategic testing programs we can collapse the cycle time for making these medically-important strains available at the dispensary and patient level<br />Cannabis testing supports the legitimization of the cannabis movement<br />© 2011 Pure Analytics, LLC<br />
  4. 4. Cannabis Testing: The Current Landscape<br />Dynamic environment<br />Prevalence of testing is on the rise<br />The cost of testing has driven dispensary choices in the past<br />New laboratories emerging every month to take advantage of the economic opportunity<br />Increasing occurrence of non-scientists offering analytical services<br />Instrument companies marketing to collectives and non-scientists to do their own testing<br />Patients and collectives are asking important questions about reliability of results they are investing in<br />© 2011 Pure Analytics, LLC<br />
  5. 5. Cannabis Testing: The Current Landscape <br />The Issue of Oversight<br />Cannabis testing is currently un-regulated<br />In a regulated environment a customer can simply choose a lab that is “certified” to an agreed set of standards<br />Method validation- Systematic verification that an analytical system is repeatable and reliable<br />Quality Control- Ongoing checks that repeated measurements agree<br />Calibration requirements-<br />Frequency<br />Acceptance criteria<br />© 2011 Pure Analytics, LLC<br />
  6. 6. Cannabis Testing- The Current Landscape<br />Factors Affecting Accuracy & Precision in Potency Testing<br />Sampling of product for testing at dispensary<br />Sampling of product at the lab<br />Scales / balances<br />Volume measurement equipment<br />Calibration of equipment<br />Use of certified standards: chemical products certified for content of THC, CBD & CBN<br />Allows results to be consistent regardless of lab and which machine is used<br />Proper maintenance of equipment<br />Side-by-side testing between facilities or third parties helps confirm accuracy<br />© 2011 Pure Analytics, LLC<br />
  7. 7. Cannabis Testing: The Current Landscape<br />Variations in methods used<br />Choice of instrument<br />GC vs HPLC or TLC<br />Within GC, FID vs TCD vs MS detection<br />Both GC and HPLC produce accurate results<br />TLC (test strips) are usually used for qualitative purposes to determine if active compounds are present, but not their amounts<br />Instrument needed depends on the application of results<br />© 2011 Pure Analytics, LLC<br />99%<br />
  8. 8. The Potency Testing Process<br />Gas Chromatography<br />High Performance Liquid Chromatography<br />A high pressure stream of solvent helps move compounds to the detector<br />System at ambient temperature<br />Detects THC, THCA, CBD, CBDA <br />Can detect cannabinoid acids<br />Analysis produces a lot of solvent waste<br />A low pressure stream of gas helps move the compounds to the detector<br />Heated system<br />Detects total available THC, CBD and CBN<br />Cannot detect cannabinoid acids<br />Analysis does not produce significant waste<br />© 2011 Pure Analytics, LLC<br />
  9. 9. Cannabis Testing: The Current Landscape<br />In the current non-regulated environment the consumer has to be savvy and know what questions to ask a prospective cannabis lab<br />10 Questions for a prospective cannabis lab:<br />What training or expertise do you have to be able to perform cannabinoid analysis?<br />What is your method for testing cannabis? What are the limitations of your selected method?<br />Which cannabinoids do you test for? Do you have reliable reference standards for all of them?<br />Analytical methods need to be ‘validated’ before you can be sure they are fully reliable. Have you done this already, and how did you do this. Did it include a third party? If you didn’t do it yet, how can I be sure my results will be accurate?<br />How is CBN related to THC, and why is it important to test for it? <br />© 2011 Pure Analytics, LLC<br />
  10. 10. Cannabis Testing: The Current Landscape<br />10 Questions for a prospective cannabis lab, con’t:<br />Are you aware of acidic cannabinoids? In samples such as edibles and tincture they can be present at high levels. How do you deal with that? <br />What is the average THC/CBD content your lab has measured? <br />Did your lab ever test the same cannabis twice, with very different results? What was the explanation for that, and what has been changed to prevent it from happening again?<br />What kind of samples do you test (flowers, edibles, tincture)? Have you optimized your extraction and analysis protocol for each kind of sample?<br />What do you do with left-over samples?<br />Contributors: A. Hazekamp, J. Raber, N. Palmer, E. Taylor, P. Morris, S. Miller<br />© 2011 Pure Analytics, LLC<br />
  11. 11. Applications of Potency Testing<br />Collapsing the Cycle Time for Developing CBD-Rich Cannabis<br />© 2011 Pure Analytics, LLC<br />
  12. 12. Developing CBD-rich Cannabis<br />Potency testing can accelerate the development of CBD-rich varieties of cannabis through <br />Isolation of genetics of interest<br />Strategic hybrid crosses supported by early-stage growth testing<br />Characterization of final product<br />© 2011 Pure Analytics, LLC<br />
  13. 13. Developing CBD-Rich Cannabis<br />First step- identification of CBD-rich strains<br />With the availability of potency testing several CBD-rich strains have been identified, Cannatonic, Sour Tsunami, Harlequin and many more<br />Where seeds are commercially available this provides a starting point for those looking to produce CBD-rich cannabis<br />© 2011 Pure Analytics, LLC<br />
  14. 14. Developing CBD-Rich Cannabis<br />Next- isolation of a CBD-rich specimen<br />All currently available seed stock for CBD-rich strains are a mixture of high-THC, high-CBD and low-CBD genetics<br />In most cases, only 25% of those seeds are expected to yield a CBD-dominant cannabis plant<br />Strategic use of potency testing can isolate those CBD-dominant plants before the flowering stage<br />© 2011 Pure Analytics, LLC<br />
  15. 15. Developing CBD-Rich Cannabis<br />Early Stage Testing to Support Strategic Parent Selection<br />Cannabinoid profile can be characterized within the first several weeks of the emergence of the shoot from the soil<br />Botanically specific leaves are sampled and analyzed<br />The results provide an accurate profiling of the relative THC:CBD content in the flowers produced by the plant<br />Method can be applied to both males and females<br />© 2011 Pure Analytics, LLC<br />
  16. 16. Developing CBD-Rich Cannabis<br />Isolation of CBD-dominant plants during the vegetative state offers several advantages:<br />Avoid expensive and time-consuming back-cloning of all seeds by isolating only those plants of interest<br />Support selection of high-CBD parents for future crosses without waiting an extra growth cycle to determine which plants are CBD-rich through final flower testing<br />Collapse the development cycle-time for isolation or breeding of high-CBD strains down to weeks rather than months or years<br />© 2011 Pure Analytics, LLC<br />
  17. 17. Thank You Denver!<br />© 2011 Pure Analytics, LLC<br />
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