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Cannabis Testing The Current Landscape
 

Cannabis Testing The Current Landscape

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    Cannabis Testing The Current Landscape Cannabis Testing The Current Landscape Presentation Transcript

    • Cannabis Testing:The Current Landscape
      © 2011 Pure Analytics, LLC
    • Agenda
      Cannabis Testing: The Current Landscape
      The importance of testing
      The dynamic market
      The issue of oversight
      Questions patients and collectives should ask
      Applications of Potency Testing in the Development of non-THC Dominant Strains
      Identification
      Predictive crosses and collapsing the cycle time for CBD-rich strain development
      © 2011 Pure Analytics, LLC
    • Cannabis Testing: The Current Landscape
      The Importance of Testing
      Provide objective information to patients for use in selecting:
      Cannabinoid profile, THC:CBD
      Overall dosage level, THC + CBD
      Promote the availability of medically-important cannabis options such as CBD-rich varieties
      Through strategic testing programs we can collapse the cycle time for making these medically-important strains available at the dispensary and patient level
      Cannabis testing supports the legitimization of the cannabis movement
      © 2011 Pure Analytics, LLC
    • Cannabis Testing: The Current Landscape
      Dynamic environment
      Prevalence of testing is on the rise
      The cost of testing has driven dispensary choices in the past
      New laboratories emerging every month to take advantage of the economic opportunity
      Increasing occurrence of non-scientists offering analytical services
      Instrument companies marketing to collectives and non-scientists to do their own testing
      Patients and collectives are asking important questions about reliability of results they are investing in
      © 2011 Pure Analytics, LLC
    • Cannabis Testing: The Current Landscape
      The Issue of Oversight
      Cannabis testing is currently un-regulated
      In a regulated environment a customer can simply choose a lab that is “certified” to an agreed set of standards
      Method validation- Systematic verification that an analytical system is repeatable and reliable
      Quality Control- Ongoing checks that repeated measurements agree
      Calibration requirements-
      Frequency
      Acceptance criteria
      © 2011 Pure Analytics, LLC
    • Cannabis Testing- The Current Landscape
      Factors Affecting Accuracy & Precision in Potency Testing
      Sampling of product for testing at dispensary
      Sampling of product at the lab
      Scales / balances
      Volume measurement equipment
      Calibration of equipment
      Use of certified standards: chemical products certified for content of THC, CBD & CBN
      Allows results to be consistent regardless of lab and which machine is used
      Proper maintenance of equipment
      Side-by-side testing between facilities or third parties helps confirm accuracy
      © 2011 Pure Analytics, LLC
    • Cannabis Testing: The Current Landscape
      Variations in methods used
      Choice of instrument
      GC vs HPLC or TLC
      Within GC, FID vs TCD vs MS detection
      Both GC and HPLC produce accurate results
      TLC (test strips) are usually used for qualitative purposes to determine if active compounds are present, but not their amounts
      Instrument needed depends on the application of results
      © 2011 Pure Analytics, LLC
      99%
    • The Potency Testing Process
      Gas Chromatography
      High Performance Liquid Chromatography
      A high pressure stream of solvent helps move compounds to the detector
      System at ambient temperature
      Detects THC, THCA, CBD, CBDA
      Can detect cannabinoid acids
      Analysis produces a lot of solvent waste
      A low pressure stream of gas helps move the compounds to the detector
      Heated system
      Detects total available THC, CBD and CBN
      Cannot detect cannabinoid acids
      Analysis does not produce significant waste
      © 2011 Pure Analytics, LLC
    • Cannabis Testing: The Current Landscape
      In the current non-regulated environment the consumer has to be savvy and know what questions to ask a prospective cannabis lab
      10 Questions for a prospective cannabis lab:
      What training or expertise do you have to be able to perform cannabinoid analysis?
      What is your method for testing cannabis? What are the limitations of your selected method?
      Which cannabinoids do you test for? Do you have reliable reference standards for all of them?
      Analytical methods need to be ‘validated’ before you can be sure they are fully reliable. Have you done this already, and how did you do this. Did it include a third party? If you didn’t do it yet, how can I be sure my results will be accurate?
      How is CBN related to THC, and why is it important to test for it?
      © 2011 Pure Analytics, LLC
    • Cannabis Testing: The Current Landscape
      10 Questions for a prospective cannabis lab, con’t:
      Are you aware of acidic cannabinoids? In samples such as edibles and tincture they can be present at high levels. How do you deal with that?
      What is the average THC/CBD content your lab has measured?
      Did your lab ever test the same cannabis twice, with very different results? What was the explanation for that, and what has been changed to prevent it from happening again?
      What kind of samples do you test (flowers, edibles, tincture)? Have you optimized your extraction and analysis protocol for each kind of sample?
      What do you do with left-over samples?
      Contributors: A. Hazekamp, J. Raber, N. Palmer, E. Taylor, P. Morris, S. Miller
      © 2011 Pure Analytics, LLC
    • Applications of Potency Testing
      Collapsing the Cycle Time for Developing CBD-Rich Cannabis
      © 2011 Pure Analytics, LLC
    • Developing CBD-rich Cannabis
      Potency testing can accelerate the development of CBD-rich varieties of cannabis through
      Isolation of genetics of interest
      Strategic hybrid crosses supported by early-stage growth testing
      Characterization of final product
      © 2011 Pure Analytics, LLC
    • Developing CBD-Rich Cannabis
      First step- identification of CBD-rich strains
      With the availability of potency testing several CBD-rich strains have been identified, Cannatonic, Sour Tsunami, Harlequin and many more
      Where seeds are commercially available this provides a starting point for those looking to produce CBD-rich cannabis
      © 2011 Pure Analytics, LLC
    • Developing CBD-Rich Cannabis
      Next- isolation of a CBD-rich specimen
      All currently available seed stock for CBD-rich strains are a mixture of high-THC, high-CBD and low-CBD genetics
      In most cases, only 25% of those seeds are expected to yield a CBD-dominant cannabis plant
      Strategic use of potency testing can isolate those CBD-dominant plants before the flowering stage
      © 2011 Pure Analytics, LLC
    • Developing CBD-Rich Cannabis
      Early Stage Testing to Support Strategic Parent Selection
      Cannabinoid profile can be characterized within the first several weeks of the emergence of the shoot from the soil
      Botanically specific leaves are sampled and analyzed
      The results provide an accurate profiling of the relative THC:CBD content in the flowers produced by the plant
      Method can be applied to both males and females
      © 2011 Pure Analytics, LLC
    • Developing CBD-Rich Cannabis
      Isolation of CBD-dominant plants during the vegetative state offers several advantages:
      Avoid expensive and time-consuming back-cloning of all seeds by isolating only those plants of interest
      Support selection of high-CBD parents for future crosses without waiting an extra growth cycle to determine which plants are CBD-rich through final flower testing
      Collapse the development cycle-time for isolation or breeding of high-CBD strains down to weeks rather than months or years
      © 2011 Pure Analytics, LLC
    • Thank You Denver!
      © 2011 Pure Analytics, LLC