How to manage PCV2  in gilts? Dr Jean-Bernard HERIN,  Avian and Swine technical and marketing manager Dr Guillaume PERREUL...
Agenda <ul><li>PCV2 and purchased gilts: what is their status versus PCV2 infection? </li></ul><ul><li>PCV2 and gilt accli...
Material and methods <ul><li>30 nucleus farms </li></ul><ul><li>Supplying 4 French pig companies (representing 25% of Fren...
ELISA PCV2 SYNBIOTICS and interpretation <ul><li>Reproductibility: </li></ul><ul><ul><li>30% of variation is considered as...
ELISA PCV2 SYNBIOTICS:  What kind of applications? <ul><li>Gilts PCV2 antibodies status </li></ul><ul><li>Quality of vacci...
Results: company A 125 102 141 42 52 62 SD (%) 3163 4673 1624 11144 240 2077 PCV2 ELISA titre A6 A5 A4 A3 A2 A1 Farm
Results : company P 84 66 59 146 34 78 100 73 191 123 SD (%) 3145 6592 2429 1014 300 1216 100 1712 585 840 PCV2 ELISA titr...
Results: company C 138 139 63 65 66 188 114 144 SD (%) 1022 4457 3003 8949 6592 349 11 327 PCV2 ELISA titre C8 C7 C6 C5 C4...
Results: company D 0 88 104 94 187 SD (%) 15000 48 54 2611 3455 PCV2 ELISA titre D6 D5 D3 D2 D1 Farm
Low and heterogeneous titers: which consequences? <ul><li>Gilts adaptation: </li></ul><ul><ul><li>Heterogenous immune stat...
* 3 fœtuses aborted before necropsy Sow 25 : the lowest titer and the biggest pcv2 contamination in genital tract 1/10 4 1...
<ul><li>« You will have to test gilts before entering in farm, and you must not introduce naïve gilts to PCV2. </li></ul><...
Agenda <ul><li>PCV2 and purchased gilts: what is their status versus PCV2 infection? </li></ul><ul><li>PCV2 and gilt accli...
Vaccination study with Circovac at the experimental station of Guernevez Impact on acclimatization and performances of hig...
<ul><li>High microbial pressure (PCV, SIV) </li></ul><ul><li>All gilts seroconverted to PCV2 (ELISA)= infected with PCV2 <...
Material and methods   <ul><li>Animals </li></ul><ul><ul><ul><li>5 vaccinated batches (n= 82) </li></ul></ul></ul><ul><ul>...
Results: clinical monitoring in quarantine <ul><li>H+   =   Hyperthermia from 39,5 to 39,9°C.  H++  = Hyperthermia from 40...
Results: ADWG in quarantine Non vaccinated    ≈   325 g/d Vaccinated ≈  439 g/d p < 0,001 Improvement of ADWG of vaccinate...
ADWG 3 weeks after insemination  g/day Better acclimatization of gilts at entry in farm
Results: weight at the first farrowing Weight  (kg) Vaccinated Non  vaccinated p < 0,05 <ul><li>Importance of the weight a...
Total born piglets at 1 st  farrowing Non vaccinated Vaccinated +0.5 total born in  vaccinated group
Conclusion <ul><li>In quarantine: </li></ul><ul><ul><li>better gilt acclimatization: less clinical signs </li></ul></ul><u...
Improvement of reproductive parameters of gilts after Circovac vaccination Michel Noirrit DVM - France
Farm description <ul><li>1600 sows, farrow to finish, Brittany, France </li></ul><ul><li>Internal breeding, GP Topigs A pr...
Case description <ul><li>Reproduction parameters </li></ul><ul><li>  Conception rate (all sows) 95.21% </li></ul><ul><li> ...
Case description <ul><li>15 gilts introduced every week  </li></ul><ul><ul><li>in a batch of 70 sows </li></ul></ul><ul><u...
PCV2 Circovac vaccination <ul><li>Circovac vaccination on gilts  </li></ul><ul><ul><li>11/15 batches </li></ul></ul><ul><u...
Reproductive parameters before and after Circovac Non vaccinated gilts Circovac vaccinated gilts p Gilts (number) 165 165 ...
Conception rate of young sows and immunity <ul><li>Fertility rate over 95%  =  good management of AI </li></ul><ul><li>Lon...
Agenda <ul><li>PCV2 and purchased gilts: what is their status versus PCV2 infection? </li></ul><ul><li>PCV2 and gilt accli...
PORFIMAD study <ul><li>Comparative study on reproductive parameters: </li></ul><ul><ul><li>10 vaccinated farms </li></ul><...
Total born/litter  Born alive/litter Vaccinated Non vaccinated + 0,5   Parity + 0,4 Parity
Conclusion <ul><li>In this study:    live born piglets from young sows </li></ul><ul><li>Field studies all over the world...
Discussion <ul><li>Low infection rate in gilts in nucleus farms:  </li></ul><ul><ul><li>Successful management in genetic f...
<ul><li>Thanks   for your attention! </li></ul>
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02 perreul relu 13 avril soir

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  • A l’abattoir : ganglions + oviductes ligaturées L’occasion de faire aussi un diagnostic différentiel Virémie truie : détectable sur 4 truies parmi les 6 infectées (donc pas toutes) et que pendant 3 semaines environ Pour lecture de ce tableau : Les colonnes : on voit que dans le groupe témoin, on ne retrouve pas de pcv2 Les lignes : on voit une inconstance dans les zones où pcv2 est retrouvé, mais se dégagent majoritairement : ganglion et fœtus Immunité à évoquer
  • 02 perreul relu 13 avril soir

    1. 1. How to manage PCV2 in gilts? Dr Jean-Bernard HERIN, Avian and Swine technical and marketing manager Dr Guillaume PERREUL, Swine technical manager
    2. 2. Agenda <ul><li>PCV2 and purchased gilts: what is their status versus PCV2 infection? </li></ul><ul><li>PCV2 and gilt acclimatization: benefit of PCV2 vaccination: </li></ul><ul><ul><li>purchased gilts </li></ul></ul><ul><ul><li>self replacement gilts </li></ul></ul><ul><li>Circovac and results at first farrowing </li></ul>
    3. 3. Material and methods <ul><li>30 nucleus farms </li></ul><ul><li>Supplying 4 French pig companies (representing 25% of French pigs) </li></ul><ul><li>No PCV2 vaccination on gilts </li></ul><ul><li>5 gilts/farm sampled on testing </li></ul><ul><li>Lab analysis performed: ELISA PCV2 (Synbiotics test) </li></ul>
    4. 4. ELISA PCV2 SYNBIOTICS and interpretation <ul><li>Reproductibility: </li></ul><ul><ul><li>30% of variation is considered as normal for this technic </li></ul></ul><ul><li>Seroconversion: </li></ul><ul><ul><li>Paired sera </li></ul></ul><ul><ul><li>PCV2 titre should double </li></ul></ul><ul><li>Objective: to assess antibodies level homogeneity (like PPV) </li></ul>Forum d’Athènes
    5. 5. ELISA PCV2 SYNBIOTICS: What kind of applications? <ul><li>Gilts PCV2 antibodies status </li></ul><ul><li>Quality of vaccination: </li></ul><ul><ul><li>sows </li></ul></ul><ul><ul><li>piglets: colotrum intake assessment only </li></ul></ul><ul><li>PCV2 infection </li></ul><ul><ul><li>Seroconversion </li></ul></ul><ul><li>Fetal exposure to PCV2: </li></ul><ul><ul><li>reproductive failures </li></ul></ul><ul><ul><li>thoracical and abdominal fluid </li></ul></ul>Forum d’Athènes
    6. 6. Results: company A 125 102 141 42 52 62 SD (%) 3163 4673 1624 11144 240 2077 PCV2 ELISA titre A6 A5 A4 A3 A2 A1 Farm
    7. 7. Results : company P 84 66 59 146 34 78 100 73 191 123 SD (%) 3145 6592 2429 1014 300 1216 100 1712 585 840 PCV2 ELISA titre P10 P9 P8 P7 P6 P5 P4 P3 P2 P1 Farm
    8. 8. Results: company C 138 139 63 65 66 188 114 144 SD (%) 1022 4457 3003 8949 6592 349 11 327 PCV2 ELISA titre C8 C7 C6 C5 C4 C3 C2 C1 Farm
    9. 9. Results: company D 0 88 104 94 187 SD (%) 15000 48 54 2611 3455 PCV2 ELISA titre D6 D5 D3 D2 D1 Farm
    10. 10. Low and heterogeneous titers: which consequences? <ul><li>Gilts adaptation: </li></ul><ul><ul><li>Heterogenous immune status at entry </li></ul></ul><ul><ul><li>Risk of PCV2 infection and circulation </li></ul></ul><ul><li>PCVD: </li></ul><ul><ul><li>PCV2 immune status of sows heterogeneous => piglets with heterogeneous status at weaning => increased risk of PCVD </li></ul></ul><ul><ul><li>PCVD control: major role of CMI </li></ul></ul><ul><ul><ul><li>Increased CMI transfered to piglets in vaccinated sows </li></ul></ul></ul><ul><li>Impact on reproductive disorders? </li></ul><ul><ul><li>Pr Sarli’s study </li></ul></ul>
    11. 11. * 3 fœtuses aborted before necropsy Sow 25 : the lowest titer and the biggest pcv2 contamination in genital tract 1/10 4 1/10 4 1/10 3 1/10 3 1/10 4 1/10 4 1/10 3 1/10 3 1/10 2 - - - - - - - - 1 Foetus and corresponding uterine tract (right) - - - + - - - - 7 Foetus and corresponding uterine tract (left) - - - - - - - 1/11 - Amniotic fluids (positive/total litter) - - - - - - 3/7 - 10/16 Foetuses (positive/total litter) - - - - + - - - - Cervix - - - / - / - - + Tracheo-bronchial lymph node - - - - - + - - + Uterine right lymph node - - - - - - - - + Uterine left lymph node - - - - - + - - - Tonsil - - - - 28 7 21 - 21 - 35 PCR PCV2 in blood at D+AI 8 0* 11 0 0 0 7 11 16 Pregnant : YES Pregnant : NO Pregnant : YES Négative sows Infected sows
    12. 12. <ul><li>« You will have to test gilts before entering in farm, and you must not introduce naïve gilts to PCV2. </li></ul><ul><li>Not to vaccinate gilts against PCV2 is a mistake.»  </li></ul><ul><li>« You have just one thing to do: vaccinate them, as you do against PPV.» </li></ul>Pr Hans NAUWYNCK DVM, PhD – Laboratory of Virology – Faculty of Veterinary Medicine – Ghent University - Belgium What should we do?
    13. 13. Agenda <ul><li>PCV2 and purchased gilts: what is their status versus PCV2 infection? </li></ul><ul><li>PCV2 and gilt acclimatization: benefit of PCV2 vaccination: </li></ul><ul><ul><li>purchased gilts </li></ul></ul><ul><ul><li>self replacement gilts </li></ul></ul><ul><li>Circovac and results at first farrowing </li></ul>
    14. 14. Vaccination study with Circovac at the experimental station of Guernevez Impact on acclimatization and performances of high health status gilts
    15. 15. <ul><li>High microbial pressure (PCV, SIV) </li></ul><ul><li>All gilts seroconverted to PCV2 (ELISA)= infected with PCV2 </li></ul><ul><li>What could you expect with only PCV2 management? </li></ul>Gilt health status in quarantine
    16. 16. Material and methods <ul><li>Animals </li></ul><ul><ul><ul><li>5 vaccinated batches (n= 82) </li></ul></ul></ul><ul><ul><ul><li>4 control batches (n=93) </li></ul></ul></ul><ul><li>Circovac vaccination </li></ul><ul><ul><ul><li>Primo vaccination of gilts: </li></ul></ul></ul><ul><ul><ul><ul><li>5 and 2 weeks before entering in quarantine </li></ul></ul></ul></ul><ul><ul><ul><li>Primo vaccination of sows : </li></ul></ul></ul><ul><ul><ul><ul><li>6 and 3 weeks before farrowing </li></ul></ul></ul></ul><ul><ul><ul><li>Booster: 3 weeks before farrowing </li></ul></ul></ul>A B
    17. 17. Results: clinical monitoring in quarantine <ul><li>H+ = Hyperthermia from 39,5 to 39,9°C. H++ = Hyperthermia from 40,0 to 40,5°C. H+++ > 40,5°C </li></ul><ul><ul><li>Figure in brackets means number days with clinical signs. </li></ul></ul><ul><li>Diar = Diarrhoea – Appe = Lack of appetite, left-overs- Anor = Anorexia (too much left-overs)- Para = Paralysis - Limp = Limping. </li></ul><ul><ul><li>Figure in brackets means number days with clinical signs. </li></ul></ul><ul><li>ND =No Defined - Death = Mortality. </li></ul><ul><ul><li>Figure in brackets means number of gilts </li></ul></ul>Non vaccinated Nothing Nothing Nothing Nothing Dia (1) Nothing Nothing Nothing 15 B 30 Nothing Anor (1) Nothing Nothing Nothing Nothing Anor (2) Nothing 15 B 29 ND ND ND Nothing Nothing Nothing Nothing Anor (1) 16 B 27 Nothing Anor (1) Nothing Nothing Nothing Nothing Nothing Nothing 18 B 26 Vaccinated 17 20 19 18 18 N Appe (1) Anor (2) Loss of weight Appe (1) Nothing Nothing Appe (2) Anor (3) B 24 Nothing Anor (4) Anor (1) Death (1) Anor (1) Nothing Appe (1) Anor (1) Appe (1) Death (1) H++ (1) Appe (1) B 23 Nothing Nothing Nothing Appe (1) Death (1) Nothing Anor (1) Appe (1) Nothing B 22 Appe (2) Anor (1) Appe (1) 5 Appe (2) Diar (2) Limp (1) 4 Appe (1) Nothing 2 Appe (1) Limp (3) Appe (1) Anor (2) 1 Appe(2) Appe (2) Diar (3) Appe (2) Appe (1) Anor (2) B 21 Anor (1) Anor (2) Nothing Appe (3) Anor (1) B 20 7 6 3 0 Week Less clinical signs in quarantine in the vaccinated batches!
    18. 18. Results: ADWG in quarantine Non vaccinated ≈ 325 g/d Vaccinated ≈ 439 g/d p < 0,001 Improvement of ADWG of vaccinated gilts!
    19. 19. ADWG 3 weeks after insemination g/day Better acclimatization of gilts at entry in farm
    20. 20. Results: weight at the first farrowing Weight (kg) Vaccinated Non vaccinated p < 0,05 <ul><li>Importance of the weight at first farrowing </li></ul><ul><li>(Rolland CARIOLET – «  paysan breton » 13th-19th June 2008) </li></ul><ul><li>Under 200 kg at first farrowing, a gilt can’t suckle more than 10 piglets </li></ul><ul><li>From 200 to 210 kg: 11 piglets </li></ul><ul><li>From 210 to 220 kg: 12 piglets </li></ul><ul><li>> 220 kg she can wean 13 piglets </li></ul>
    21. 21. Total born piglets at 1 st farrowing Non vaccinated Vaccinated +0.5 total born in vaccinated group
    22. 22. Conclusion <ul><li>In quarantine: </li></ul><ul><ul><li>better gilt acclimatization: less clinical signs </li></ul></ul><ul><ul><li>better growth </li></ul></ul><ul><li>At insemination: </li></ul><ul><ul><li>better growth </li></ul></ul><ul><li>At first farrowing: </li></ul><ul><ul><li>improvement of born piglets/ litter </li></ul></ul>Improvement all along the gilt life!
    23. 23. Improvement of reproductive parameters of gilts after Circovac vaccination Michel Noirrit DVM - France
    24. 24. Farm description <ul><li>1600 sows, farrow to finish, Brittany, France </li></ul><ul><li>Internal breeding, GP Topigs A producing Topigs 40 gilts </li></ul><ul><li>Gilts raised separately, 12 weeks in quarantine </li></ul><ul><li>No clinical signs of PMWS after weaning </li></ul><ul><li>PRRS  </li></ul><ul><ul><li>mass vaccination with PROGRESSIS ® every 3,5 months </li></ul></ul><ul><ul><li>stable situation </li></ul></ul>
    25. 25. Case description <ul><li>Reproduction parameters </li></ul><ul><li> Conception rate (all sows) 95.21% </li></ul><ul><li> Abortion rate (all sows) 3.75 % </li></ul><ul><li> Abortion rate (gilts) 7.27 % </li></ul><ul><li> Mummified foetuses 0.3/litter </li></ul><ul><li> Still born piglets 0.73/litter </li></ul><ul><ul><li>Wean to finish mortality rate 3.5 to 4.5% </li></ul></ul>
    26. 26. Case description <ul><li>15 gilts introduced every week </li></ul><ul><ul><li>in a batch of 70 sows </li></ul></ul><ul><ul><li>after PRRS, parvovirus and erysipelas vaccination in quarantine </li></ul></ul><ul><li>Individual housing up to 25 days after AI, then group housing (welfare regulation) </li></ul><ul><li>Abortions up to 80 days of pregnancy: </li></ul><ul><ul><li>PCR Leptospira, PRRSV, parvovirus: negative </li></ul></ul><ul><ul><li>PCR PCV2: positive on 4 hearts of aborted foetuses </li></ul></ul>
    27. 27. PCV2 Circovac vaccination <ul><li>Circovac vaccination on gilts </li></ul><ul><ul><li>11/15 batches </li></ul></ul><ul><ul><li>2 injections during quarantine + booster injection 3 weeks before farrowing </li></ul></ul><ul><li>Circovac mass vaccination of the sow herd </li></ul><ul><ul><li>2 injections at a 3-week-interval </li></ul></ul>
    28. 28. Reproductive parameters before and after Circovac Non vaccinated gilts Circovac vaccinated gilts p Gilts (number) 165 165 % return to oestrus 5.1 % 1.8 % 0.125 % abortion (n°) 7.3% (12) 3.6% (6) 0.146 % farrowing 83.9 % 90.5 % 0.072 Mummies/litter 0.3 0.3 Stillborn/litter 0.8 0.8 Weaning to Fecundation interval 8.7 days 5.5 days NA
    29. 29. Conception rate of young sows and immunity <ul><li>Fertility rate over 95% = good management of AI </li></ul><ul><li>Long period of adaptation </li></ul><ul><ul><li>12 weeks quarantine </li></ul></ul><ul><ul><li>PRRS stabilisation, SMEDI primo-infection </li></ul></ul><ul><li>Nevertheless: 62.5% of returns to heat on parities 1 and 2 before Circovac vaccination </li></ul><ul><li>= lack of immunity of naïve young females suspected </li></ul>PCV2 vaccination seems necessary even without clinical signs of PMWS
    30. 30. Agenda <ul><li>PCV2 and purchased gilts: what is their status versus PCV2 infection? </li></ul><ul><li>PCV2 and gilt acclimatization: benefit of PCV2 vaccination: </li></ul><ul><ul><li>purchased gilts </li></ul></ul><ul><ul><li>self replacement gilts </li></ul></ul><ul><li>CIRCOVAC and results at first farrowing </li></ul>
    31. 31. PORFIMAD study <ul><li>Comparative study on reproductive parameters: </li></ul><ul><ul><li>10 vaccinated farms </li></ul></ul><ul><ul><ul><li>sows and gilts vaccinated with Circovac for at least one year </li></ul></ul></ul><ul><ul><li>9 control farms </li></ul></ul><ul><ul><ul><li>non vaccinated </li></ul></ul></ul>28x10 6 /year 18x10 6 /year 0,9x10 6 /year 1,2 10 6 800x10 3 44x10 3
    32. 32. Total born/litter Born alive/litter Vaccinated Non vaccinated + 0,5 Parity + 0,4 Parity
    33. 33. Conclusion <ul><li>In this study:  live born piglets from young sows </li></ul><ul><li>Field studies all over the world demonstrate a significant improvement of the breeding performances after vaccination </li></ul><ul><li>Improvement on 1 st farrowing: </li></ul><ul><ul><li>it could be explained by low contact with PCV2 before entering in quarantine in some cases (naive animals) </li></ul></ul><ul><ul><li>it emphasizes the importance of gilt vaccination </li></ul></ul>
    34. 34. Discussion <ul><li>Low infection rate in gilts in nucleus farms: </li></ul><ul><ul><li>Successful management in genetic farms (no rhinitis, no PRRSV): consequently low viral pressure </li></ul></ul><ul><ul><li>But PCV2 is ubiquitous: </li></ul></ul><ul><ul><ul><li>Gilts PCV2 infected with low and heterogenous titers </li></ul></ul></ul><ul><ul><ul><li>Some naïve batches (seronegative) </li></ul></ul></ul><ul><li>Introduction of gilts in commercial farm is at risk </li></ul><ul><ul><li>Adaptation problems </li></ul></ul><ul><ul><li>PCV2 reproductive disorders may happen </li></ul></ul><ul><li>How to protect gilts? </li></ul><ul><ul><li>In nucleus farms: vaccination before entering in quarantine </li></ul></ul><ul><ul><li>In production farms: </li></ul></ul><ul><ul><ul><li>Routine PCV2 sow vaccination as parvovirus (2 ml, twice) </li></ul></ul></ul><ul><ul><ul><li>Booster vaccination before the first farrowing (2 ml) </li></ul></ul></ul><ul><ul><ul><li>Role of boar </li></ul></ul></ul>
    35. 35. <ul><li>Thanks for your attention! </li></ul>
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