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02 perreul relu 13 avril soir
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02 perreul relu 13 avril soir

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  • A l’abattoir : ganglions + oviductes ligaturées L’occasion de faire aussi un diagnostic différentiel Virémie truie : détectable sur 4 truies parmi les 6 infectées (donc pas toutes) et que pendant 3 semaines environ Pour lecture de ce tableau : Les colonnes : on voit que dans le groupe témoin, on ne retrouve pas de pcv2 Les lignes : on voit une inconstance dans les zones où pcv2 est retrouvé, mais se dégagent majoritairement : ganglion et fœtus Immunité à évoquer
  • Transcript

    • 1. How to manage PCV2 in gilts? Dr Jean-Bernard HERIN, Avian and Swine technical and marketing manager Dr Guillaume PERREUL, Swine technical manager
    • 2. Agenda
      • PCV2 and purchased gilts: what is their status versus PCV2 infection?
      • PCV2 and gilt acclimatization: benefit of PCV2 vaccination:
        • purchased gilts
        • self replacement gilts
      • Circovac and results at first farrowing
    • 3. Material and methods
      • 30 nucleus farms
      • Supplying 4 French pig companies (representing 25% of French pigs)
      • No PCV2 vaccination on gilts
      • 5 gilts/farm sampled on testing
      • Lab analysis performed: ELISA PCV2 (Synbiotics test)
    • 4. ELISA PCV2 SYNBIOTICS and interpretation
      • Reproductibility:
        • 30% of variation is considered as normal for this technic
      • Seroconversion:
        • Paired sera
        • PCV2 titre should double
      • Objective: to assess antibodies level homogeneity (like PPV)
      Forum d’Athènes
    • 5. ELISA PCV2 SYNBIOTICS: What kind of applications?
      • Gilts PCV2 antibodies status
      • Quality of vaccination:
        • sows
        • piglets: colotrum intake assessment only
      • PCV2 infection
        • Seroconversion
      • Fetal exposure to PCV2:
        • reproductive failures
        • thoracical and abdominal fluid
      Forum d’Athènes
    • 6. Results: company A 125 102 141 42 52 62 SD (%) 3163 4673 1624 11144 240 2077 PCV2 ELISA titre A6 A5 A4 A3 A2 A1 Farm
    • 7. Results : company P 84 66 59 146 34 78 100 73 191 123 SD (%) 3145 6592 2429 1014 300 1216 100 1712 585 840 PCV2 ELISA titre P10 P9 P8 P7 P6 P5 P4 P3 P2 P1 Farm
    • 8. Results: company C 138 139 63 65 66 188 114 144 SD (%) 1022 4457 3003 8949 6592 349 11 327 PCV2 ELISA titre C8 C7 C6 C5 C4 C3 C2 C1 Farm
    • 9. Results: company D 0 88 104 94 187 SD (%) 15000 48 54 2611 3455 PCV2 ELISA titre D6 D5 D3 D2 D1 Farm
    • 10. Low and heterogeneous titers: which consequences?
      • Gilts adaptation:
        • Heterogenous immune status at entry
        • Risk of PCV2 infection and circulation
      • PCVD:
        • PCV2 immune status of sows heterogeneous => piglets with heterogeneous status at weaning => increased risk of PCVD
        • PCVD control: major role of CMI
          • Increased CMI transfered to piglets in vaccinated sows
      • Impact on reproductive disorders?
        • Pr Sarli’s study
    • 11. * 3 fœtuses aborted before necropsy Sow 25 : the lowest titer and the biggest pcv2 contamination in genital tract 1/10 4 1/10 4 1/10 3 1/10 3 1/10 4 1/10 4 1/10 3 1/10 3 1/10 2 - - - - - - - - 1 Foetus and corresponding uterine tract (right) - - - + - - - - 7 Foetus and corresponding uterine tract (left) - - - - - - - 1/11 - Amniotic fluids (positive/total litter) - - - - - - 3/7 - 10/16 Foetuses (positive/total litter) - - - - + - - - - Cervix - - - / - / - - + Tracheo-bronchial lymph node - - - - - + - - + Uterine right lymph node - - - - - - - - + Uterine left lymph node - - - - - + - - - Tonsil - - - - 28 7 21 - 21 - 35 PCR PCV2 in blood at D+AI 8 0* 11 0 0 0 7 11 16 Pregnant : YES Pregnant : NO Pregnant : YES Négative sows Infected sows
    • 12.
      • « You will have to test gilts before entering in farm, and you must not introduce naïve gilts to PCV2.
      • Not to vaccinate gilts against PCV2 is a mistake.» 
      • « You have just one thing to do: vaccinate them, as you do against PPV.»
      Pr Hans NAUWYNCK DVM, PhD – Laboratory of Virology – Faculty of Veterinary Medicine – Ghent University - Belgium What should we do?
    • 13. Agenda
      • PCV2 and purchased gilts: what is their status versus PCV2 infection?
      • PCV2 and gilt acclimatization: benefit of PCV2 vaccination:
        • purchased gilts
        • self replacement gilts
      • Circovac and results at first farrowing
    • 14. Vaccination study with Circovac at the experimental station of Guernevez Impact on acclimatization and performances of high health status gilts
    • 15.
      • High microbial pressure (PCV, SIV)
      • All gilts seroconverted to PCV2 (ELISA)= infected with PCV2
      • What could you expect with only PCV2 management?
      Gilt health status in quarantine
    • 16. Material and methods
      • Animals
          • 5 vaccinated batches (n= 82)
          • 4 control batches (n=93)
      • Circovac vaccination
          • Primo vaccination of gilts:
            • 5 and 2 weeks before entering in quarantine
          • Primo vaccination of sows :
            • 6 and 3 weeks before farrowing
          • Booster: 3 weeks before farrowing
      A B
    • 17. Results: clinical monitoring in quarantine
      • H+ = Hyperthermia from 39,5 to 39,9°C. H++ = Hyperthermia from 40,0 to 40,5°C. H+++ > 40,5°C
        • Figure in brackets means number days with clinical signs.
      • Diar = Diarrhoea – Appe = Lack of appetite, left-overs- Anor = Anorexia (too much left-overs)- Para = Paralysis - Limp = Limping.
        • Figure in brackets means number days with clinical signs.
      • ND =No Defined - Death = Mortality.
        • Figure in brackets means number of gilts
      Non vaccinated Nothing Nothing Nothing Nothing Dia (1) Nothing Nothing Nothing 15 B 30 Nothing Anor (1) Nothing Nothing Nothing Nothing Anor (2) Nothing 15 B 29 ND ND ND Nothing Nothing Nothing Nothing Anor (1) 16 B 27 Nothing Anor (1) Nothing Nothing Nothing Nothing Nothing Nothing 18 B 26 Vaccinated 17 20 19 18 18 N Appe (1) Anor (2) Loss of weight Appe (1) Nothing Nothing Appe (2) Anor (3) B 24 Nothing Anor (4) Anor (1) Death (1) Anor (1) Nothing Appe (1) Anor (1) Appe (1) Death (1) H++ (1) Appe (1) B 23 Nothing Nothing Nothing Appe (1) Death (1) Nothing Anor (1) Appe (1) Nothing B 22 Appe (2) Anor (1) Appe (1) 5 Appe (2) Diar (2) Limp (1) 4 Appe (1) Nothing 2 Appe (1) Limp (3) Appe (1) Anor (2) 1 Appe(2) Appe (2) Diar (3) Appe (2) Appe (1) Anor (2) B 21 Anor (1) Anor (2) Nothing Appe (3) Anor (1) B 20 7 6 3 0 Week Less clinical signs in quarantine in the vaccinated batches!
    • 18. Results: ADWG in quarantine Non vaccinated ≈ 325 g/d Vaccinated ≈ 439 g/d p < 0,001 Improvement of ADWG of vaccinated gilts!
    • 19. ADWG 3 weeks after insemination g/day Better acclimatization of gilts at entry in farm
    • 20. Results: weight at the first farrowing Weight (kg) Vaccinated Non vaccinated p < 0,05
      • Importance of the weight at first farrowing
      • (Rolland CARIOLET – «  paysan breton » 13th-19th June 2008)
      • Under 200 kg at first farrowing, a gilt can’t suckle more than 10 piglets
      • From 200 to 210 kg: 11 piglets
      • From 210 to 220 kg: 12 piglets
      • > 220 kg she can wean 13 piglets
    • 21. Total born piglets at 1 st farrowing Non vaccinated Vaccinated +0.5 total born in vaccinated group
    • 22. Conclusion
      • In quarantine:
        • better gilt acclimatization: less clinical signs
        • better growth
      • At insemination:
        • better growth
      • At first farrowing:
        • improvement of born piglets/ litter
      Improvement all along the gilt life!
    • 23. Improvement of reproductive parameters of gilts after Circovac vaccination Michel Noirrit DVM - France
    • 24. Farm description
      • 1600 sows, farrow to finish, Brittany, France
      • Internal breeding, GP Topigs A producing Topigs 40 gilts
      • Gilts raised separately, 12 weeks in quarantine
      • No clinical signs of PMWS after weaning
      • PRRS 
        • mass vaccination with PROGRESSIS ® every 3,5 months
        • stable situation
    • 25. Case description
      • Reproduction parameters
      • Conception rate (all sows) 95.21%
      • Abortion rate (all sows) 3.75 %
      • Abortion rate (gilts) 7.27 %
      • Mummified foetuses 0.3/litter
      • Still born piglets 0.73/litter
        • Wean to finish mortality rate 3.5 to 4.5%
    • 26. Case description
      • 15 gilts introduced every week
        • in a batch of 70 sows
        • after PRRS, parvovirus and erysipelas vaccination in quarantine
      • Individual housing up to 25 days after AI, then group housing (welfare regulation)
      • Abortions up to 80 days of pregnancy:
        • PCR Leptospira, PRRSV, parvovirus: negative
        • PCR PCV2: positive on 4 hearts of aborted foetuses
    • 27. PCV2 Circovac vaccination
      • Circovac vaccination on gilts
        • 11/15 batches
        • 2 injections during quarantine + booster injection 3 weeks before farrowing
      • Circovac mass vaccination of the sow herd
        • 2 injections at a 3-week-interval
    • 28. Reproductive parameters before and after Circovac Non vaccinated gilts Circovac vaccinated gilts p Gilts (number) 165 165 % return to oestrus 5.1 % 1.8 % 0.125 % abortion (n°) 7.3% (12) 3.6% (6) 0.146 % farrowing 83.9 % 90.5 % 0.072 Mummies/litter 0.3 0.3 Stillborn/litter 0.8 0.8 Weaning to Fecundation interval 8.7 days 5.5 days NA
    • 29. Conception rate of young sows and immunity
      • Fertility rate over 95% = good management of AI
      • Long period of adaptation
        • 12 weeks quarantine
        • PRRS stabilisation, SMEDI primo-infection
      • Nevertheless: 62.5% of returns to heat on parities 1 and 2 before Circovac vaccination
      • = lack of immunity of naïve young females suspected
      PCV2 vaccination seems necessary even without clinical signs of PMWS
    • 30. Agenda
      • PCV2 and purchased gilts: what is their status versus PCV2 infection?
      • PCV2 and gilt acclimatization: benefit of PCV2 vaccination:
        • purchased gilts
        • self replacement gilts
      • CIRCOVAC and results at first farrowing
    • 31. PORFIMAD study
      • Comparative study on reproductive parameters:
        • 10 vaccinated farms
          • sows and gilts vaccinated with Circovac for at least one year
        • 9 control farms
          • non vaccinated
      28x10 6 /year 18x10 6 /year 0,9x10 6 /year 1,2 10 6 800x10 3 44x10 3
    • 32. Total born/litter Born alive/litter Vaccinated Non vaccinated + 0,5 Parity + 0,4 Parity
    • 33. Conclusion
      • In this study:  live born piglets from young sows
      • Field studies all over the world demonstrate a significant improvement of the breeding performances after vaccination
      • Improvement on 1 st farrowing:
        • it could be explained by low contact with PCV2 before entering in quarantine in some cases (naive animals)
        • it emphasizes the importance of gilt vaccination
    • 34. Discussion
      • Low infection rate in gilts in nucleus farms:
        • Successful management in genetic farms (no rhinitis, no PRRSV): consequently low viral pressure
        • But PCV2 is ubiquitous:
          • Gilts PCV2 infected with low and heterogenous titers
          • Some naïve batches (seronegative)
      • Introduction of gilts in commercial farm is at risk
        • Adaptation problems
        • PCV2 reproductive disorders may happen
      • How to protect gilts?
        • In nucleus farms: vaccination before entering in quarantine
        • In production farms:
          • Routine PCV2 sow vaccination as parvovirus (2 ml, twice)
          • Booster vaccination before the first farrowing (2 ml)
          • Role of boar
    • 35.
      • Thanks for your attention!

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