02 perreul
Upcoming SlideShare
Loading in...5
×

Like this? Share it with your network

Share
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Be the first to comment
    Be the first to like this
No Downloads

Views

Total Views
616
On Slideshare
616
From Embeds
0
Number of Embeds
0

Actions

Shares
Downloads
7
Comments
0
Likes
0

Embeds 0

No embeds

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
    No notes for slide
  • A l’abattoir : ganglions + oviductes ligaturées L’occasion de faire aussi un diagnostic différentiel Virémie truie : détectable sur 4 truies parmi les 6 infectées (donc pas toutes) et que pendant 3 semaines environ Pour lecture de ce tableau : Les colonnes : on voit que dans le groupe témoin, on ne retrouve pas de pcv2 Les lignes : on voit une inconstance dans les zones où pcv2 est retrouvé, mais se dégagent majoritairement : ganglion et fœtus Immunité à évoquer

Transcript

  • 1. How to manage PCV2 in gilts? Dr Jean-Bernard HERIN, Avian and Swine technical and marketing manager Dr Guillaume PERREUL, Swine technical manager
  • 2. Agenda
    • PCV2 and purchased gilts: what is their status versus PCV2 infection?
    • PCV2 and gilt acclimatization: benefit of PCV2 vaccination:
      • purchased gilts
      • self replacement gilts
    • Circovac and results at first farrowing
  • 3. Material and methods
    • 30 nucleus farms
    • Supplying 4 French pig companies (representing 25% of French pigs)
    • No PCV2 vaccination on gilts
    • 5 gilts/farm sampled on testing
    • Lab analysis performed: ELISA PCV2 (Synbiotics test)
  • 4. ELISA PCV2 SYNBIOTICS and interpretation
    • Reproductibility:
      • 30% of variation is considered as normal for this technic
    • Seroconversion:
      • Paired sera
      • PCV2 titre should double
    • Objective: to assess antibodies level homogeneity (like PPV)
    Forum d’Athènes
  • 5. ELISA PCV2 SYNBIOTICS: What kind of applications?
    • Gilts PCV2 antibodies status
    • Quality of vaccination:
      • sows
      • piglets: colotrum intake assessment only
    • PCV2 infection
      • Seroconversion
    • Fetal exposure to PCV2:
      • reproductive failures
      • thoracical and abdominal fluid
    Forum d’Athènes
  • 6. Results: company A 125 102 141 42 52 62 SD (%) 3163 4673 1624 11144 240 2077 PCV2 ELISA titre A6 A5 A4 A3 A2 A1 Farm
  • 7. Results : company P 84 66 59 146 34 78 100 73 191 123 SD (%) 3145 6592 2429 1014 300 1216 100 1712 585 840 PCV2 ELISA titre P10 P9 P8 P7 P6 P5 P4 P3 P2 P1 Farm
  • 8. Results: company C 138 139 63 65 66 188 114 144 SD (%) 1022 4457 3003 8949 6592 349 11 327 PCV2 ELISA titre C8 C7 C6 C5 C4 C3 C2 C1 Farm
  • 9. Results: company D 0 88 104 94 187 SD (%) 15000 48 54 2611 3455 PCV2 ELISA titre D6 D5 D3 D2 D1 Farm
  • 10. Low and heterogeneous titers: which consequences?
    • Gilts adaptation:
      • Heterogenous immune status at entry
      • Risk of PCV2 infection and circulation
    • PCVD:
      • PCV2 immune status of sows heterogeneous => piglets with heterogeneous status at weaning => increased risk of PCVD
      • PCVD control: major role of CMI
        • Increased CMI transfered to piglets in vaccinated sows
    • Impact on reproductive disorders?
      • Pr Sarli’s study
  • 11. * 3 fœtuses aborted before necropsy Sow 25 : the lowest titer and the biggest pcv2 contamination in genital tract 1/10 4 1/10 4 1/10 3 1/10 3 1/10 4 1/10 4 1/10 3 1/10 3 1/10 2 - - - - - - - - 1 Foetus and corresponding uterine tract (right) - - - + - - - - 7 Foetus and corresponding uterine tract (left) - - - - - - - 1/11 - Amniotic fluids (positive/total litter) - - - - - - 3/7 - 10/16 Foetuses (positive/total litter) - - - - + - - - - Cervix - - - / - / - - + Tracheo-bronchial lymph node - - - - - + - - + Uterine right lymph node - - - - - - - - + Uterine left lymph node - - - - - + - - - Tonsil - - - - 28 7 21 - 21 - 35 PCR PCV2 in blood at D+AI 8 0* 11 0 0 0 7 11 16 Pregnant : YES Pregnant : NO Pregnant : YES Négative sows Infected sows
  • 12.
    • « You will have to test gilts before entering in farm, and you must not introduce naïve gilts to PCV2.
    • Not to vaccinate gilts against PCV2 is a mistake.» 
    • « You have just one thing to do: vaccinate them, as you do against PPV.»
    Pr Hans NAUWYNCK DVM, PhD – Laboratory of Virology – Faculty of Veterinary Medicine – Ghent University - Belgium What should we do?
  • 13. Agenda
    • PCV2 and purchased gilts: what is their status versus PCV2 infection?
    • PCV2 and gilt acclimatization: benefit of PCV2 vaccination:
      • purchased gilts
      • self replacement gilts
    • Circovac and results at first farrowing
  • 14. Vaccination study with Circovac at the experimental station of Guernevez Impact on acclimatization and performances of high health status gilts
  • 15.
    • High microbial pressure (PCV, SIV)
    • All gilts seroconverted to PCV2 (ELISA)= infected with PCV2
    • What could you expect with only PCV2 management?
    Gilt health status in quarantine
  • 16. Material and methods
    • Animals
        • 5 vaccinated batches (n= 82)
        • 4 control batches (n=93)
    • Circovac vaccination
        • Primo vaccination of gilts:
          • 5 and 2 weeks before entering in quarantine
        • Primo vaccination of sows :
          • 6 and 3 weeks before farrowing
        • Booster: 3 weeks before farrowing
    A B
  • 17. Results: clinical monitoring in quarantine
    • H+ = Hyperthermia from 39,5 to 39,9°C. H++ = Hyperthermia from 40,0 to 40,5°C. H+++ > 40,5°C
      • Figure in brackets means number days with clinical signs.
    • Diar = Diarrhoea – Appe = Lack of appetite, left-overs- Anor = Anorexia (too much left-overs)- Para = Paralysis - Limp = Limping.
      • Figure in brackets means number days with clinical signs.
    • ND =No Defined - Death = Mortality.
      • Figure in brackets means number of gilts
    Non vaccinated Nothing Nothing Nothing Nothing Dia (1) Nothing Nothing Nothing 15 B 30 Nothing Anor (1) Nothing Nothing Nothing Nothing Anor (2) Nothing 15 B 29 ND ND ND Nothing Nothing Nothing Nothing Anor (1) 16 B 27 Nothing Anor (1) Nothing Nothing Nothing Nothing Nothing Nothing 18 B 26 Vaccinated 17 20 19 18 18 N Appe (1) Anor (2) Loss of weight Appe (1) Nothing Nothing Appe (2) Anor (3) B 24 Nothing Anor (4) Anor (1) Death (1) Anor (1) Nothing Appe (1) Anor (1) Appe (1) Death (1) H++ (1) Appe (1) B 23 Nothing Nothing Nothing Appe (1) Death (1) Nothing Anor (1) Appe (1) Nothing B 22 Appe (2) Anor (1) Appe (1) 5 Appe (2) Diar (2) Limp (1) 4 Appe (1) Nothing 2 Appe (1) Limp (3) Appe (1) Anor (2) 1 Appe(2) Appe (2) Diar (3) Appe (2) Appe (1) Anor (2) B 21 Anor (1) Anor (2) Nothing Appe (3) Anor (1) B 20 7 6 3 0 Week Less clinical signs in quarantine in the vaccinated batches!
  • 18. Results: ADWG in quarantine Non vaccinated ≈ 325 g/d Vaccinated ≈ 439 g/d p < 0,001 Improvement of ADWG of vaccinated gilts!
  • 19. ADWG 3 weeks after insemination g/day Better acclimatization of gilts at entry in farm
  • 20. Results: weight at the first farrowing Weight (kg) Vaccinated Non vaccinated p < 0,05
    • Importance of the weight at first farrowing
    • (Rolland CARIOLET – «  paysan breton » 13th-19th June 2008)
    • Under 200 kg at first farrowing, a gilt can’t suckle more than 10 piglets
    • From 200 to 210 kg: 11 piglets
    • From 210 to 220 kg: 12 piglets
    • > 220 kg she can wean 13 piglets
  • 21. Total born piglets at 1 st farrowing Non vaccinated Vaccinated +0.5 total born in vaccinated group
  • 22. Conclusion
    • In quarantine:
      • better gilt acclimatization: less clinical signs
      • better growth
    • At insemination:
      • better growth
    • At first farrowing:
      • improvement of born piglets/ litter
    Improvement all along the gilt life!
  • 23. Improvement of reproductive parameters of gilts after Circovac vaccination Michel Noirrit DVM - France
  • 24. Farm description
    • 1600 sows, farrow to finish, Brittany, France
    • Internal breeding, GP Topigs A producing Topigs 40 gilts
    • Gilts raised separately, 12 weeks in quarantine
    • No clinical signs of PMWS after weaning
    • PRRS 
      • mass vaccination with PROGRESSIS ® every 3,5 months
      • stable situation
  • 25. Case description
    • Reproduction parameters
    • Conception rate (all sows) 95.21%
    • Abortion rate (all sows) 3.75 %
    • Abortion rate (gilts) 7.27 %
    • Mummified foetuses 0.3/litter
    • Still born piglets 0.73/litter
      • Wean to finish mortality rate 3.5 to 4.5%
  • 26. Case description
    • 15 gilts introduced every week
      • in a batch of 70 sows
      • after PRRS, parvovirus and erysipelas vaccination in quarantine
    • Individual housing up to 25 days after AI, then group housing (welfare regulation)
    • Abortions up to 80 days of pregnancy:
      • PCR Leptospira, PRRSV, parvovirus: negative
      • PCR PCV2: positive on 4 hearts of aborted foetuses
  • 27. PCV2 Circovac vaccination
    • Circovac vaccination on gilts
      • 11/15 batches
      • 2 injections during quarantine + booster injection 3 weeks before farrowing
    • Circovac mass vaccination of the sow herd
      • 2 injections at a 3-week-interval
  • 28. Reproductive parameters before and after Circovac Non vaccinated gilts Circovac vaccinated gilts p Gilts (number) 165 165 % return to oestrus 5.1 % 1.8 % 0.125 % abortion (n°) 7.3% (12) 3.6% (6) 0.146 % farrowing 83.9 % 90.5 % 0.072 Mummies/litter 0.3 0.3 Stillborn/litter 0.8 0.8 Weaning to Fecundation interval 8.7 days 5.5 days NA
  • 29. Conception rate of young sows and immunity
    • Fertility rate over 95% = good management of AI
    • Long period of adaptation
      • 12 weeks quarantine
      • PRRS stabilisation, SMEDI primo-infection
    • Nevertheless: 62.5% of returns to heat on parities 1 and 2 before Circovac vaccination
    • = lack of immunity of naïve young females suspected
    PCV2 vaccination seems necessary even without clinical signs of PMWS
  • 30. Agenda
    • PCV2 and purchased gilts: what is their status versus PCV2 infection?
    • PCV2 and gilt acclimatization: benefit of PCV2 vaccination:
      • purchased gilts
      • self replacement gilts
    • CIRCOVAC and results at first farrowing
  • 31. PORFIMAD study
    • Comparative study on reproductive parameters:
      • 10 vaccinated farms
        • sows and gilts vaccinated with Circovac for at least one year
      • 9 control farms
        • non vaccinated
    28x10 6 /year 18x10 6 /year 0,9x10 6 /year 1,2 10 6 800x10 3 44x10 3
  • 32. Total born/litter Born alive/litter Vaccinated Non vaccinated + 0,5 Parity + 0,4 Parity
  • 33. Conclusion
    • In this study:  live born piglets from young sows
    • Field studies all over the world demonstrate a significant improvement of the breeding performances after vaccination
    • Improvement on 1 st farrowing:
      • it could be explained by low contact with PCV2 before entering in quarantine in some cases (naive animals)
      • it emphasizes the importance of gilt vaccination
  • 34. Discussion
    • Low infection rate in gilts in nucleus farms:
      • Successful management in genetic farms (no rhinitis, no PRRSV): consequently low viral pressure
      • But PCV2 is ubiquitous:
        • Gilts PCV2 infected with low and heterogenous titers
        • Some naïve batches (seronegative)
    • Introduction of gilts in commercial farm is at risk
      • Adaptation problems
      • PCV2 reproductive disorders may happen
    • How to protect gilts?
      • In nucleus farms: vaccination before entering in quarantine
      • In production farms:
        • Routine PCV2 sow vaccination as parvovirus (2 ml, twice)
        • Booster vaccination before the first farrowing (2 ml)
        • Role of boar
  • 35.
    • Thanks for your attention!