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MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
MedicalValley Talks am 14.04.2011 mit Prof. Fabry
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MedicalValley Talks am 14.04.2011 mit Prof. Fabry

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Videomitschnitt zum Medical Valley Talk am 14.04.2011. Details und Downloads unter https://www.medical-valley-emn.de/node/2536

Videomitschnitt zum Medical Valley Talk am 14.04.2011. Details und Downloads unter https://www.medical-valley-emn.de/node/2536

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  • 1. Physikalische Ansätze zur Diagnostik  und Therapie von Tumoren Ben Fabry Institut für Kondensierte Materie Department für PhysikZentrum für Physikalisch‐Medizinische Technik
  • 2. Metastasisprimary tumorspread of tumor cells invasion transendothelial migration adhesion transport through  blood or lymph  vessels  transendothelial  migration invasion secondary tumor
  • 3. Cell crawling and migration in 2-D • CSK dynamics • adhesion • de-adhesion Crawling Neutrophil Chasing a Bacterium (D Rogers)(Alberts B. et al., Molecular Biology of the Cell) Resisting forces of the surroundings = zero 
  • 4. Cell invasion is governed by a force balance adhesion receptorde-adhesion expression bond-stability • protrusive forces • traction forces CSK-fluidity • CSK resistive forces CSK-stiffness CSK-dynamics enzymatic matrix • matrix resistive forces degradation matrix stiffness 2 µm
  • 5. Strain energy around an invaded MDA-MB-231 cell0 3.5 fJ/(10 µm)³
  • 6. Mean Squared Displacement 5 Ballistic (slope 2) 10 4 10 Super-diffusive (slope 1.3) 3 10 Diffusive (slope 1)MSD (µm2) 2 10 1 10 0 10 0 1 2 3 10 10 10 10 t (min)
  • 7. Automatic measurement of the invasion profile
  • 8. General characteristic features of the invasion profile
  • 9. Classification of tumor cell lines
  • 10. Gene expression profiles non‐invasive invasive MDA‐MB‐453 MDA‐MB‐231 MDA‐MB‐231 SW480 MS751 MCF‐7 786‐0 EJ‐28 A375increasedaveragedecreased talin laminin urokinase Matrix Adhesion positive correlation integrin alpha 3 collagen IV Deadhesion integrin alpha 5 integrin beta 3 Focal Adhesion ADAM9 Matrix proteinases TIMP1 integrin‐linked kinase (ILK) vinculin ADAM17 MMP14 calmodulin 3  microtubule‐associated protein (MAPRE2) MMP2 negative correlation keratin 18  Bauer et al, Int J Cancer 2007
  • 11. Cell invasion assay Effect of drugs Influence of gene expression profile Cell types Understand the mechanism of metastasis formation  Adhesion receptors  Matrix proteinases  Forces / cell contractility  Cytoskeletal mechanics and dynamics

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