Longitudinal scintigraphic study of parotid and submandibular gland function after total body irradiation at  bone marrow ...
©  Copyright: Mats Bågesund, DDS, PhD. No material published in this file may be reproduced in any way without written per...
Regions of interest © Mats Bågesund
Time-activity curve showing scintigraphic variables 15 30 45 60 Time from injection (minutes) % of dose injected 0 Fu US T...
Percentage secretion S%=100% x (Ma-Mi) / Ma Time from injection % of dose injected Ma Mi S% © Mats Bågesund
Objective <ul><li>To study </li></ul><ul><li>the scintigraphic functional changes over time </li></ul><ul><li>of the parot...
Patients examined <ul><li>Before After  After  TBI/BMT 3 months  12 months   n=9 n=5 n=3 </li></ul><ul><li>Sex 1F, 8M 1F, ...
Patients examined <ul><li>Before After  After  TBI/BMT 3 months  12 months Diagnose  n=9 n=5 n=3 </li></ul><ul><li>Lymphom...
<ul><li>Conditioning </li></ul><ul><li>Cyclophosphamide </li></ul><ul><li>10 Gy TBI </li></ul><ul><li>GVH-prophylaxis </li...
Method <ul><li>Whole salivary secretion rate </li></ul><ul><li>Unstimulated (USSR) 15 minutes </li></ul><ul><li>Chewing st...
Subtraction of background radiation Bågesund et al. Dentomaxillofac Radiol 2000; 29: 264-71. © Mats Bågesund
Statistical method <ul><li>Wilcoxon Signed Rank Test </li></ul>© Mats Bågesund
Unstimulated (USSR) and stimulated (SSSR) salivary secretion rate (n=5) 0 1 2 ml/min months after TBI / BMT 0 3 12 USSR SS...
Changes in salivary secretion rate after TBI/BMT  <ul><li>0 vs. 3 months after </li></ul><ul><li>USSR (n=7) P=0.018 </li><...
Scintigraphic differences in parotid glands  before vs. 3 months after TBI/BMT 15 30 45 60 Time from injection (minutes) %...
Parotid secretion (S%Par) 0 100 Before TBI/BMT 3 months after % of maximal uptake 79% 51% P=0.043 n=5 © Mats Bågesund
Parotid secretion (S%Par) % of maximal uptake Months after TBI/BMT n=3 0 100 0 3 12 0% © Mats Bågesund
Scintigraphic differences in submandibular glands   before vs. 3 months after TBI/BMT 15 30 45 60 Time from injection (min...
Submandibular secretion (S%Sub) 0 100 Before TBI/BMT 3 months after % of maximal uptake 95% 44% P=0.043 n=5 © Mats Bågesund
Submandibular secretion (S%Sub) % of maximal uptake Months after TBI/BMT n=3 0 100 0 3 12 93% © Mats Bågesund
Conclusion <ul><li>The excretion capacity (S%) is reduced three months after TBI/BMT in both parotid and submandibular gla...
<ul><li>Swedish Children’s Cancer Foundation </li></ul><ul><li>Swedish Society of Pediatric Dentistry </li></ul><ul><li>AC...
<ul><ul><li>References: </li></ul></ul><ul><li>Bågesund M , Richter S, Ågren B, Dahllöf G. Correlation between quantitativ...
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Longitudinal scintigraphic study of parotid and submandibular

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  • Abstract No. 1077. J Dent Res 2002; 81 Special Issue A (San Diego Abstracts): A-154. The possibility to treat patients with previously leading disorder, is continuously improving. Several treatments anyhow result in undesired side-effects. We have previously presented reports regarding whole salivary secretion rates showing that a reduction is found during the first year after pediatric BMT. For children treated with TBI the reduction I still persistent four years after BMT.
  • Scintigraphy is a method to study the functional capacity of the glands in different aspects. A gammacamera – in front of the patient head – measures the activity of injected tracer 99mm-technetium-pertechnetate that can be followed in specific regions of interest (ROI).
  • The data can be replayed to construct a time-activity-curve (TAC) illustrating the changes over time regarding activity in each ROI. Scintigraphic variables of specific interest are shown in this slide….
  • … ..and the important percentage stimulated secretion (sometimes called emptying capacity or excretion capacity) is defined as the percentage reduction of activity from maximum uptake (Ma) to minimum uptake level (Mi) after stimulation.
  • The objective was to study the scintigraphic functional changes over time of the parotid and submandibular glands after TBI at BMT.
  • Only nine of the patients invited accepted to participate in the scintigraphic study. Three patients did not want to continue the scintigraphic study after the first examination (before TBI) and one patient died before three month follow up. Making it a total of five patients participating at the three months follow up. Another two patients died before the twelve month follow up, making it only three patients completing the scintigraphic study.
  • The patients were treated for lymphoma, acute myeloid leukemia, chronic myeloid leukemia, acute lymphoblastic leukemia and several aplastic anemia.
  • The treatments included conditioning therapy with cyclophosphamide, 10 Gy TBI, and graft-versus-host (GVH)-prophylaxis with Methotrexate and Cyclosporine-A.
  • Unstimulated (USSR) and stimulated (SSSR) salivary secretion rates were measured during 15 and 5 minutes, respectively. Thereafter salivary gland scintigraphy (SGS) was performed during 60 minutes.
  • Background subtraction was performed using the temporal region for the parotid glands and the region superior to the thyroid gland for the submandibular glands.
  • Wilcoxon Signed Rank Test was used for statistical evaluation. (As another method was used in the abstract the p-values are differing from the abstract).
  • The USSR fell from 0,5 ml/min to 0,1 ml/min after treatment and did not increase thereafter. SSSR fell from 1,8 ml/min before to 0,2 ml/min three months after TBI/BMT and 0,7 ml/min after twelve months.
  • The changes were significant between all examinations – except for that no recovery of USSR was present after three months.
  • Regarding the parotid glands we found that scintigraphic differences were found before versus three months after treatment for Percentage stimulated secretion (S%), which decreased and Time to Maximum uptake (TMa), which was delayed.
  • The parotid emptying capacity (S%) decreased significantly from 79% before to 51% three months after treatment.
  • For the three patients completing the study no recovery (0%) of lost emptying capacity (S%) was obtained between three and twelve months after treatment.
  • Regarding the submandibular glands there were differences before versus three months after TBI/BMT for the shown variables Ma (maximal uptake) decreased and Mi (minimal uptake) increased, resulting in a reduced emptying capacity (S%). The final uptake level increased.
  • The emptying capacity in the submandibular glands decreased from 95% before TBI to 44% three months later.
  • For the three patients completing the study there was an almost total recovery (93%) of the emptying capacity at twelve months compared to the emptying capacity lost during the first three months of the study.
  • We conclude that the emptying capacity (or excretion capacity) was reduced three months after TBI/BMT in both parotid and submandibular glands. The capacity to recover lost emptying capacity seem to be present in submandibular glands, but not in parotid glands. The reduced recovering capacity of the parotid glands is a possible contributing factor to the reduced whole salivary secretion rate seen after TBI at BMT.
  • Transcript of "Longitudinal scintigraphic study of parotid and submandibular"

    1. 1. Longitudinal scintigraphic study of parotid and submandibular gland function after total body irradiation at bone marrow transplantation Mats Bågesund 1,2 Sven Richter 3 Göran Dahllöf 2 1 Center for Orthodontics and Pedodontics, Linköping 2 Department of Pediatric Dentistry, Karolinska Institutet, Stockholm 3 Department of Nuclear Medicine, Huddinge University Hospital, Karolinska Institutet, Sweden
    2. 2. © Copyright: Mats Bågesund, DDS, PhD. No material published in this file may be reproduced in any way without written permission from the author ! Address: Mats Bågesund DDS PhD Center for Orthodontics and Pedodontics SE-581 85 Linköping SWEDEN Phone: +46 13 22 88 30 Fax: +46 13 22 88 36 E-mail: [email_address]
    3. 3. Regions of interest © Mats Bågesund
    4. 4. Time-activity curve showing scintigraphic variables 15 30 45 60 Time from injection (minutes) % of dose injected 0 Fu US TMa RS DS Ma Mi S% © Mats Bågesund
    5. 5. Percentage secretion S%=100% x (Ma-Mi) / Ma Time from injection % of dose injected Ma Mi S% © Mats Bågesund
    6. 6. Objective <ul><li>To study </li></ul><ul><li>the scintigraphic functional changes over time </li></ul><ul><li>of the parotid and submandibular glands </li></ul><ul><li>in children and young adults during one year </li></ul><ul><li>after total body irradiation (TBI) </li></ul><ul><li>at bone marrow transplantation (BMT) </li></ul>© Mats Bågesund
    7. 7. Patients examined <ul><li>Before After After TBI/BMT 3 months 12 months n=9 n=5 n=3 </li></ul><ul><li>Sex 1F, 8M 1F, 4M 3M </li></ul><ul><li>Mean age (years) 13.9 15.4 17.3 </li></ul><ul><li>Range 8-28 8-28 9-29 </li></ul>© Mats Bågesund
    8. 8. Patients examined <ul><li>Before After After TBI/BMT 3 months 12 months Diagnose n=9 n=5 n=3 </li></ul><ul><li>Lymphoma 2 2 2 </li></ul><ul><li>AML 1 1 1 </li></ul><ul><li>CML 1 1 </li></ul><ul><li>ALL 4 1 </li></ul><ul><li>SAA 1 </li></ul>© Mats Bågesund
    9. 9. <ul><li>Conditioning </li></ul><ul><li>Cyclophosphamide </li></ul><ul><li>10 Gy TBI </li></ul><ul><li>GVH-prophylaxis </li></ul><ul><li>Methotrexate </li></ul><ul><li>Cyclosporine A </li></ul>© Mats Bågesund
    10. 10. Method <ul><li>Whole salivary secretion rate </li></ul><ul><li>Unstimulated (USSR) 15 minutes </li></ul><ul><li>Chewing stimulated (SSSR) 5 minutes </li></ul><ul><li>Salivary gland scintigraphy 60 minutes </li></ul>© Mats Bågesund
    11. 11. Subtraction of background radiation Bågesund et al. Dentomaxillofac Radiol 2000; 29: 264-71. © Mats Bågesund
    12. 12. Statistical method <ul><li>Wilcoxon Signed Rank Test </li></ul>© Mats Bågesund
    13. 13. Unstimulated (USSR) and stimulated (SSSR) salivary secretion rate (n=5) 0 1 2 ml/min months after TBI / BMT 0 3 12 USSR SSSR © Mats Bågesund
    14. 14. Changes in salivary secretion rate after TBI/BMT <ul><li>0 vs. 3 months after </li></ul><ul><li>USSR (n=7) P=0.018 </li></ul><ul><li>SSSR (n=7) P=0.018 </li></ul><ul><li>0 vs. 12 months after </li></ul><ul><li>USSR (n=5) P=0.043 </li></ul><ul><li>SSSR (n=5) P=0.043 </li></ul><ul><li>3 vs. 12 months after </li></ul><ul><li>SSSR (n=5) P=0.043 </li></ul>© Mats Bågesund
    15. 15. Scintigraphic differences in parotid glands before vs. 3 months after TBI/BMT 15 30 45 60 Time from injection (minutes) % of dose injected 0 TMa S% P=0.043 n=5 © Mats Bågesund
    16. 16. Parotid secretion (S%Par) 0 100 Before TBI/BMT 3 months after % of maximal uptake 79% 51% P=0.043 n=5 © Mats Bågesund
    17. 17. Parotid secretion (S%Par) % of maximal uptake Months after TBI/BMT n=3 0 100 0 3 12 0% © Mats Bågesund
    18. 18. Scintigraphic differences in submandibular glands before vs. 3 months after TBI/BMT 15 30 45 60 Time from injection (minutes) % of dose injected 0 Fu Ma Mi S% P=0.043 n=5 © Mats Bågesund
    19. 19. Submandibular secretion (S%Sub) 0 100 Before TBI/BMT 3 months after % of maximal uptake 95% 44% P=0.043 n=5 © Mats Bågesund
    20. 20. Submandibular secretion (S%Sub) % of maximal uptake Months after TBI/BMT n=3 0 100 0 3 12 93% © Mats Bågesund
    21. 21. Conclusion <ul><li>The excretion capacity (S%) is reduced three months after TBI/BMT in both parotid and submandibular glands. </li></ul><ul><li>The capacity to recover function is obvious in the submandibular glands, but is hardly present in the parotid glands one year after TBI/BMT. </li></ul><ul><li>A reduced recovering capacity of the parotid glands is a contributing factor to the reduced whole salivary secretion rate seen one year after TBI/BMT. </li></ul>© Mats Bågesund
    22. 22. <ul><li>Swedish Children’s Cancer Foundation </li></ul><ul><li>Swedish Society of Pediatric Dentistry </li></ul><ul><li>ACTA research and travel foundation </li></ul><ul><li>Mölnlycke Toiletries AB / Cederroth </li></ul><ul><li>Public Dental Health Service Östergötland </li></ul>Sponsors © Mats Bågesund
    23. 23. <ul><ul><li>References: </li></ul></ul><ul><li>Bågesund M , Richter S, Ågren B, Dahllöf G. Correlation between quantitative salivary gland scintigraphy and salivary secretion rates in children and young adults treated for hematological, malignant and metabolic diseases. Dentomaxillofac Radiol 2000; 29 : 264-271. </li></ul><ul><li>Bågesund M , Richter S, Ågren B, Ringdén O, Dahllöf G. Scintigraphic study of the major salivary glands in pediatric bone marrow transplant recipients. Bone Marrow Transplant 2000; 26 : 775-779. </li></ul><ul><li>Bågesund M , Winiarski J, Dahllöf G. Subjective xerostomia in long-term surviving children and adolescents after pediatric bone marrow transplantation. Transplantation 2000; 69: 822-826. </li></ul><ul><li>Dahllöf G, Bågesund M , Ringdén O. Impact of conditioning regimens on salivary function, caries associated microorganisms and dental caries in children treated with bone marrow transplantation. A four-year longitudinal study. Bone Marrow Transplant 1997; 20 : 479-483. </li></ul><ul><li>Dahllöf G, Bågesund M , Remberger M, Ringdén O. Risk factors for salivary gland dysfunction in children 1 year after bone marrow transplantation. Eur J Cancer Oral Oncol 1997; 33 : 327-331. </li></ul>© Mats Bågesund
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