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PhotosynthesisLight Dependent Reactions      Dr. Mark A. McGinleyHonors College and Department of       Biological Science...
Purpose of Photosynthesis• Photosynthesis converts electromagnetic  energy in light to potential energy (sometimes  called...
Two Stages of Photosynthesis• Convert electromagnetic energy in light to  potential energy in ATP and NADPH  – Light Depen...
Where Does Photosynthesis Occur?                  • Most                    photosynthesis                    takes places...
Cloroplast
Photosystem
Component of a Photosystem• Antennae Pigments  – Chlorophyll a, b and carotene  – 200 – 300 per photosystem• Reaction Cent...
Chlorophyll
What Happens in a Photosystem?• Antennae pigment absorbs a photon of light  energy  – Electron excited to higher energy le...
What Happens in a Photosystem?• Eventually energy released by one excited  electron is used to excite an electron in the  ...
What Happens in a Photosystem?             Summary• Light energy is converted into potential energy  that is stored in an ...
Why Is This Important• Potential energy in excited electron can be  used to do work!!!
Electron Flow• Two patterns of electron flow  – Cyclic electron flow  – Non-cyclic electron flow
Cyclic Electron Flow
Electron Flow• Excited electron in 1o electron acceptor moves  to adjacent molecule• Electron drops to lower energy level•...
Cyclic Electron Flow• As the name suggests, the excited electron is  eventually returned to the reaction center  chlorophy...
Importance of the H+ Concentration             Gradient• The active transport of H+ inside of the  thylakoid space produce...
Chemiosmosis
Cyclic Electron Flow                   Summary• Excited electron from 1o electron acceptor is  moves from molecule to mole...
Photosystems• Turns out there are two types of photosystems   – Photosystem I and Photosystem II   – Photosystems are name...
Photosystems• Cyclic Flow  – PS I• Non-cyclic Flow  – Both PS I and PS II
Non-cyclic FlowInvolves both photosystems. Begins in PS IIand then involves PS I
Non-cyclic Flow• Starts in PS II   – Electron from P700 passed to 1o electron acceptor   – excited electron undergoes elec...
Non-cyclic Flow• In PS I, this electron that originated in PS II is  re-excited to an even higher energy level   – Excited...
Non-cyclic Flow        Do You See the Problem??• The electron from P700 in PS II used to help  produce NADPH  – Thus P700 ...
Non-cyclic Flow Summary• Energy from two photons of light energy are  converted into potential energy in ATP and  NADPH• W...
Non-cyclic Flow-
Light Dependent Reactions               Summary• Both cyclic and non-cyclic flow are occurring  simultaneously in the same...
The Light Dependent Reactions            • Light dependent reactions              involves molecules              imbedded...
What’s Next!• The ATP and NADPH produced during the light  dependent reactions will be used to power  the light dependent ...
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Photosynthesis light dependent reactions

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  • On Slide 23, is it supposed to be P680 where it says P700 and vice versa since it starts in PSII?
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Transcript of "Photosynthesis light dependent reactions"

  1. 1. PhotosynthesisLight Dependent Reactions Dr. Mark A. McGinleyHonors College and Department of Biological Sciences Texas Tech University
  2. 2. Purpose of Photosynthesis• Photosynthesis converts electromagnetic energy in light to potential energy (sometimes called chemical energy) stored in chemical bonds of glucose – The potential energy in glucose can be stored and moved to allow it to be used when and where it is needed
  3. 3. Two Stages of Photosynthesis• Convert electromagnetic energy in light to potential energy in ATP and NADPH – Light Dependent Reactions• Convert potential energy in ATP and NADPH to potential energy stored in glucose – Light Independent Reactions
  4. 4. Where Does Photosynthesis Occur? • Most photosynthesis takes places in leaves –Palisade and spongy mesophyll cells • Organelles known as chloroplasts
  5. 5. Cloroplast
  6. 6. Photosystem
  7. 7. Component of a Photosystem• Antennae Pigments – Chlorophyll a, b and carotene – 200 – 300 per photosystem• Reaction Center – Chlorophyll a• Primary Electron Acceptor
  8. 8. Chlorophyll
  9. 9. What Happens in a Photosystem?• Antennae pigment absorbs a photon of light energy – Electron excited to higher energy level • Potential energy in excited electron – When electron falls back to resting stage, the energy that is released is used to excited an electron in an adjacent antennae pigment • resonance
  10. 10. What Happens in a Photosystem?• Eventually energy released by one excited electron is used to excite an electron in the reaction center – Chlorophyll a• This excited electron does not fall back to resting stage• Instead, the excited electron (and its potential energy) moves to an adjacent molecule know as the Primary Electron acceptor – 1o electron acceptor has “extra” electron – Reaction center is missing one electron
  11. 11. What Happens in a Photosystem? Summary• Light energy is converted into potential energy that is stored in an excited electron that is passed form the reaction center to the primary electron acceptor• Energetic results – Light energy converted into potential energy in excited electron
  12. 12. Why Is This Important• Potential energy in excited electron can be used to do work!!!
  13. 13. Electron Flow• Two patterns of electron flow – Cyclic electron flow – Non-cyclic electron flow
  14. 14. Cyclic Electron Flow
  15. 15. Electron Flow• Excited electron in 1o electron acceptor moves to adjacent molecule• Electron drops to lower energy level• Energy released• Energy used to actively transport H+ from stroma into the thylakoid space – Causes a H+ concentration gradient
  16. 16. Cyclic Electron Flow• As the name suggests, the excited electron is eventually returned to the reaction center chlorophyll that originally lost it.
  17. 17. Importance of the H+ Concentration Gradient• The active transport of H+ inside of the thylakoid space produces a H+ concentration gradient• This concentration gradient powers a process known as Chemiosmosis that converts ADP to ATP – Potential energy stored in the chemical bonds of ATP
  18. 18. Chemiosmosis
  19. 19. Cyclic Electron Flow Summary• Excited electron from 1o electron acceptor is moves from molecule to molecule and eventually returns to the reaction center that lost it• Energetic Result – Potential energy stored in excited electron is converted to potential energy stored in ATP
  20. 20. Photosystems• Turns out there are two types of photosystems – Photosystem I and Photosystem II – Photosystems are named based on the order that they were discovered • PS I discovered before PS II• PS I and PS II differ slightly in the absorption spectra of the Chlorophyll a molecule – PS I P700 – PS II P680
  21. 21. Photosystems• Cyclic Flow – PS I• Non-cyclic Flow – Both PS I and PS II
  22. 22. Non-cyclic FlowInvolves both photosystems. Begins in PS IIand then involves PS I
  23. 23. Non-cyclic Flow• Starts in PS II – Electron from P700 passed to 1o electron acceptor – excited electron undergoes electron flow • Similar to in cyclic flow • H+ concentration gradient powers chemiosmosis-> ATP – excited electron does not return to P700 that lost it • Instead, that electron and its remaining potential energy is transferred to P680 in the reaction center of PS I
  24. 24. Non-cyclic Flow• In PS I, this electron that originated in PS II is re-excited to an even higher energy level – Excited electron undergoes a different pattern of electron flow – Result of this flow is that energy in excited electron is converted into potential energy in NADPH
  25. 25. Non-cyclic Flow Do You See the Problem??• The electron from P700 in PS II used to help produce NADPH – Thus P700 is missing an electron• P700 recovers its missing electron by taking an electron from water (H20) – H+ and O2 released
  26. 26. Non-cyclic Flow Summary• Energy from two photons of light energy are converted into potential energy in ATP and NADPH• Water is broken down to release H+ and O2
  27. 27. Non-cyclic Flow-
  28. 28. Light Dependent Reactions Summary• Both cyclic and non-cyclic flow are occurring simultaneously in the same chloroplasts• Energetic result- light energy is converted into potential energy in ATP and NADPH• Chemical result- H20 => H+ + O2
  29. 29. The Light Dependent Reactions • Light dependent reactions involves molecules imbedded in the thylakoid membrane • Allows the cell to precisely control the spatial organization of molecules – Electron transport and resonance can not occur if molecules are floating in cytoplasm
  30. 30. What’s Next!• The ATP and NADPH produced during the light dependent reactions will be used to power the light dependent reactions of photosynthesis
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