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Vibrio mahadi ppt


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  • 1. Sharq Elneil CollegeSchool of Medical Laboratory Sciences Department of Microbiology Medical Bacteriology course Vibrios U.Mahadi Hassan Mahmoud Bsc, Msc, MIBMS Microbiology
  • 2. 3 Important Genera: Vibrio Aeromonas, Plesiomonas Oxidase +ve
  • 3. Medically important speciesVibrio choleraeVibrio paraheamolyticusVibrio vulnificus
  • 4. Clinical Syndromesspecies Vibrio –Vibrio cholerae 01 (This is a specific powerful serotype) • Mild diarrhea to severe deadly diarrhea –Depends on how much toxin produced • Vomiting can also occur • Lose 10-25 liters a day, sometimes 1 liter/hour
  • 5. • Lose 50% of body weight during course of disease• Death within hours (renal failure and shock)• Self-limiting, but have to replace fluids and electrolytes or high likelihood of eventual death Vibrio cholerae non01 (less potent serotype)• Much milder diarrhea, not life- threatening
  • 6. Vibrio speciesClinical Syndromes Vibrio parahaemolyticus •Seafood-associated •Seen mostly in coastal regions •Mild to moderate diarrhea •Self-limiting disease, patients recover uneventful
  • 7. Vibrio vulnificusDiarrhea associated with raw oystersA more threatening infection is when patients get a wound infection contaminated with seawaterThe wound infection progresses rapidly and can cause death within a day.
  • 8. Morphology Gram negative curved rod, measuring3–4 0.5 μm Have a single flagellum at one end.In smears made from solid cultures, the vibriosmay appear less The use of dilute carbon fuchsin(1 in 10) is recommended as a counterstain in theGram techniquewhen staining Vibrio species. V. cholerae 0139, unlike V. cholerae 01, iscapsulated surrounded by a thickcapsule.
  • 9. I. V. cholera O1: – Agglutinate in Antisera Directed Against O1 Antigen. – Produce Enterotoxin.2. Non-Toxigenic V. cholera O1:3. Non O1 V. cholera (NCV, NAV):II. V. cholerae O139
  • 10. Vibrio choleraeGram-negative, curved rods
  • 11. Vibrio choleraeLeifson flagella stain
  • 12. Vibrio choleraecurved rods with polar flagellum (EM)
  • 13. V. cholerae O1--------------------------------------------------- Biotye El-tor ClassicHaemolysis (SRBC) + --VP + --Resistance to PB + -----------------------------------------------------
  • 14. VIRULENCE FACTORS: o Enterotoxin: CT o Adherence: Ability to Attach to the Microvilli at the Brush Borders of the Epithelial Cells.EPIDEMIOLOGY: o Causes Widespread Disease. o Human Carriers: o Modern International
  • 15. PATHOGENESIS & CLINICALINFECTION:  Infectious Dose: Incubation period: Abrupt Onset of Diarrhoea & Vomiting Stool > Watery (Rice Water), Odorless Fatality Rate:
  • 16. LAB. DIAGNOSIS: Isolation & Identification of the Organism. Media: TCBS Transport Media:
  • 17. TREATMENT: Prompt Replacement of Fluid & Elecrolytes Tetracycline and others
  • 18. PREVENTION & CONTROL: Adequate Sewage Treatment and Water Purification Systems. Vaccination:
  • 19. Typical Vibrio choleracontaminated water supply
  • 20. Vibrio parahaemolyticusoHalophilic vibrioo Associated with seafoodo Gastroenteritis: oOutbreaks of Food Poisoningo Extraintestinal infections > woundsoVirulence Factors: oHaemolysin > oheat-stable cytotoxin oHeat-labile enterotoxin oAdherence to human intestinal cells
  • 21. Aeromonas speciesA. hydrophila, A. sobria & A.caviae Enterotoxins, Cytotoxins, Haemolysins, Etc… Gastroenteritis, Septicaemia, and Skin & Wound InfectionsAntimicrobial Suscebtibilitytesting: TMP-SMX, Tetracycline, Gentamicin Resistant to b-Lactam Antibiotics.