Bls 107 general microbiology

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  • 1. Structure of bacterial cell
  • 2. Cell wall Gram(+) cell wall Gram(-) cell wall thick peptidoglycane  thin peptidoglycane  no teichoic acids teichoic acid, lipoteichoic acid  outer membrane - .molecular screen.  integral proteins polysaccharides  Porins  permeases,  translocation proteins  protein receptors  lipopolysaccharide LPS = endotoxin (negative charge, hydrophobic, anti-phagocytotic, toxic)  lipid A  core polysaccharides  O-antigen
  • 3. Gram stain method
  • 4. Cell wall:Peptidoglycane or murein network-like polymere surrounds the cell like net bag many layers very rigid Gram(+): lysozyme may break protoplast Gram(-): penicillin may break spheroplast L-form
  • 5. Flagella-for motilitylong spiral protein chains-flagellin
  • 6. Position Of flagella
  • 7. Pili- for attachment;- for changing ofDNA betweenbacteria (sex ofbacteria).