Detection of receptors for Fc of Ig or complement components (C3b and C3d receptors) by rosettle formation with antibody coated eryrothcytes (EA- rosetes) or antibody-complement – coated erythrocytes (EAC-rosettles)
Immunofluorescence & autoradiography
Bacterial adherence to lymphocytes (in conjuction with fluoresent antibody technique).
Helix prometia antigen chracterizes bovine and equine T-lymphocytes
Surface Ig is a reliable marker for B cells, but is labile and requires care Ig marker is to be differentiated from T-cells (few), macrophages, suppressor macrophages, suppressor cells (CD8+) and helper T-cells (CD8),
CD8+ and CD4+ cells binds sIg via Fc receptors.
Rosetting B cells produces higher values for B cells than B cells identified by surface markers.
Fig. 3: Human blood: Nucleus of the neutrophil (a) has not constricted into distinct lobes. Neutrophils with more or less band‑form of nucleus are called band neutrophils and are more immature than the lobed or segmented neutrophils. Band neutrophils comprise 3 to 5 % of leukocytes in normal blood. Blood platelets (b) and a small lymphocyte (c). Wrights stain x 400