Onion root tip – cell count Task #1 Now that you are able to identify cells in different stages of the cell cycle, now it is time to understand how long a cell will tend to be at each of these stages. The task is to count how many cells in your root tip specimen were ‘frozen in time’ in different stages of division when this slide was made. Tip 1 –For cells that do not appear to be in a stage of division, if there is no clearly defined nucleus, disregard the cell. If the nuclear envelope is clearly defined then this can be counted as a cell in interphase. Tip 2 - The most efficient way to conduct this part of the experiment is if one member of your group scans the sample whilst the other member keeps a tally of the cells cited. Stage of Interphase Prophase Metaphase Anaphase Telophase Total MitosisCell countPercentage 100% Task #2 This is your homework task Calculate the percentage of the total number of cells for each stage of the cell cycle and then put this information in to an Excel spreadsheet. Use this spreadsheet to create a pie chart, and bring this to next class for analysis. Discussion In which phase of the cycle did you find most cells? Based on your knowledge of what the cell is doing at each stage can you explain your findings?