2013 HID University Introduction
 

2013 HID University Introduction

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2013 HID University Introduction given at the Life Technologies Forensic Seminar Series

2013 HID University Introduction given at the Life Technologies Forensic Seminar Series

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2013 HID University Introduction 2013 HID University Introduction Presentation Transcript

  • 1Life Technologies™ | Introduction: Increasing Success Rates & Efficiency from Crime Scene to Courtroom Ariana Wheaton Senior Market Development Manager - Americas Life Technologies
  • 2Life Technologies™ | Increased  Efficiency   Ease  of  Use   Success  with   Difficult  Samples   Simplified   Interpreta;on   Higher   Throughput   Streamlined   Workflow   Technology  Improvements  Over  Last  7  Years…     Increased  Integra9on  and  Efficiency  
  • 3Life Technologies™ |    Time  to  Result     (hrs/per  24  samples)   ~1-­‐3.5  hrs   ~0-­‐2  hrs   ~4  hrs   ~1.5  hrs   ~1  hrs   Improved  Efficiency  for  Single  Source  Sample  Processing        Time  to  Result     (per  24  samples)   0   0   ~40  mins   ~35  mins   ~5-­‐10  mins   Total  Time  to  Result  =  ~7-­‐12  hrs   Collec;on   Extrac;on   Quan;ta;on/   Normaliza;on   Amplifica;on   Detec;on   Analysis   Total  Time  to  Result  =  ~1.5  hrs  
  • 4Life Technologies™ | Total  Time  to  Result  =  ~10-­‐58  hrs    Sample     Prepara;on   Quan;ta;on/   Normaliza;on   Amplifica;on   Detec;on   Analysis      Time  to  Result     (hrs/per  6-­‐16  samples)   ~1-­‐48  hrs   ~2  hrs   ~3.5  hrs     ~0.75  hrs   ~2-­‐3  hrs  Time  to  Result     (hrs/per  13  samples)   ~1-­‐3  hrs   ~1.5  hrs     ~1.5  hrs   ~0.55  hrs   ~1-­‐2  hrs   Total  Time  to  Result  =  ~6-­‐9  hrs   Dependent  on   sample  type   and  method   Improved  Efficiency  for  Casework  Sample  Processing  
  • 5Life Technologies™ | A  lot  of  progress…  But  how  much  have  we   increased  your  casework  success  rates?   Increased  Efficiency   Ease  of  Use   Success  with   Difficult  Samples   Simplified   Interpreta;on   Higher   Throughput   Streamlined   Workflow   Technology  Improvements  Over  Last  7  Years…  
  • 6Life Technologies™ | Results from recent Forensic Magazine survey… Failure rate following Amplification/CE Overall average from North American Crime Labs (n=126) §  20% - Note: Labs processing higher % of property crimes and touch DNA have much higher failure rates. Ø  We did a similar survey in 2006, and the average failure rate was also 20%! Ø  Survey indicated failure rates have not been reduced, but this is due to increase in property crime processing and more challenging samples (i.e. touch samples)
  • 7Life Technologies™ | What is the cost of failed samples? §  2006 study* in the USA estimates processing a single piece of evidence through a DNA workflow takes on average: §  157.6 minutes of analyst and labor time §  Anywhere between $425 and $1,720 §  In addition, outsourcing labs typically charge the same flat fee for service whether or not the submitted sample yields probative or useful information *Source: Cost Study of DNA Testing and Analysis North Carolina DOJ OSBM March 2006 Increasing success rates in the lab means more efficient use of investigative and laboratory resources
  • 8Life Technologies™ | What contributes to the total processing cost for a DNA evidence sample? 0%   10%   20%   30%   40%   50%   60%   70%   80%   90%   100%   Typical  Cost  Breakout*   Labor  Cost/Time   Facility  Overhead   Shipping     Reagents/Supplies   Most   impacted   by    1st  Pass   Success   Rate   *Actual cost breakout will vary by laboratory
  • 9Life Technologies™ | The “Hidden Cost” of Low First Pass Success Rates Higher  1st  Pass   Success  Rate  =   Less  Re-­‐runs   Lower  1st  Pass   Success  Rate  =   More  Re-­‐runs   Reduced  Labor  &  Consumable  Cost   Increased  Labor  &  Consumable  Cost  
  • 10Life Technologies™ | Detection & Analysis Our Mission: Improve Efficiency. Increase Success Rates. Provide More Info. LIFE Offers Complete Integrated HID Workflow GeneMapper® ID-X Expert System Software Next Generation STR Kits 3500/3500xL Genetic Analyzer 3130/3130xl Genetic Analyzer Extraction Quantification Amplification 7500 Real-Time PCR Instrument Next Generation Quantifiler® Kits PrepFiler™ Forensic DNA Extraction kit Veriti® 96-Well Thermal Cycler GeneAmp® PCR System 9700 Cycler AutoMate Express™ Collection Copan 4N6FLOQSwab™ & NUCLEIC-CARD™ Screening ParaDNA® Converge™ Software DNA Workflow Kinship and Paternity Direct Search Familial Search NEW NEW NEW NEW Converge™ Software NEW NEW
  • 11Life Technologies™ | Strategies for Increased Downstream Success Rates: Improve Collection & Sample Selection Collection 4N6FLOQSwabs™ Patented technology to maximize DNA collection and elution efficiency; enables significantly improved profile success rates compared to cotton swabs Screening Sample to Answer Casework Screening Device to help determine which samples contain the highest likelihood of generating a probative DNA profile in a laboratory workflow
  • 12Life Technologies™ | NUCLEIC-CARD™ System—Now Available Globally §  Scheduled toA reliable device for collection, preservation, and long-term storage of nucleic acids Chemically-treated matrix to enable cell lysis and protein denaturation NUCLEIC –CARD™ NUCLEIC –CARD™ COLOR NUCLEIC –CARD™ Collection Device
  • 13Life Technologies™ | Quantification – Areas for Improvement   Predic9on  of  M:F  Mixture  Ra9o   Improve  Sensi9vity  and  Reproducibility   Predict  Sample  Quality   Match  Performance  to  Newer  Genera9on  STR  kits   Improve  DNA  Standard  Consistency   Faster  Time  to  Result  
  • 14Life Technologies™ | Quantifiler® Kits Product Family   Quantifiler® Duo Kit Quantifiler® Human Kit Quantifiler® Y Human Kit Three kits – for different applications • Quantifies DNA from wide variety of sources • Works with wide variety of DNA input • Sample Extraction chemistry compatible • Guides selection of optimal AmpFSTR® PCR Amplification Kit • Accurate, Sensitive & Specific Next Gen Quantification   Two new Quantifiler® Kits in 2013 + Flexible workflow software To integrate with Next Generation STR kits Quantifiler® HP Kit Quantifiler® Trio Kit HID RT v1.2 Software NEW
  • 15Life Technologies™ | The GlobalFiler™ Kit: Going Further for Casework
  • 16Life Technologies™ | Additional 6-dye Application: Yfiler® Plus Kit
  • 17Life Technologies™ | Fall 2013 Yfiler® Plus: The First 6-Dye, 27 Marker Y-STR Multiplex Plus in Power of Discrimination •  All 17 loci amplified by the Yfiler® kit •  10 new Y-STR loci including 7 Rapidly Mutating1 Y-STRs for ability to discriminate between close male relatives •  11 mini STRs below 220bp Plus in Performance •  Improved sensitivity & results with challenging casework samples •  Improved recovery of alleles and intracolor balance in male/female mixtures containing high female background •  Improved resolution capacity for male/male mixtures •  Expanded allelic ladder •  Shorter amplification times •  Direct amplification protocol with single-source samples 1.  Rapidly Mutating Y-STRs have mutation rates above 1 x 10-2 per marker per generation. 17 Yfiler Y-STRs show mutation rates between 1 x 10-3 and 1 x 10-4 per marker per generation.  The content provided herein may relate to products that have not been officially released and is subject to change without notice.
  • 18Life Technologies™ | Ion Torrent™ Semiconductor Sequencing for Forensic Applications Casework Single Source
  • 19Life Technologies™ | Mitochondrial   DNA  Sequencing   SNP  Genotyping   Paternity,  Mixtures,  Missing   Persons,  DVI   Ancestral  &  Phenotypic   SNPs  for  Inves;ga;ve  Leads   Eye  Color     Hair  Color     Missing  Persons,  DVI   Facial   Reconstruc;on   Looking  ahead…   Poten9al  PGM™  Human  Iden9fica9on  Applica9ons  
  • 20Life Technologies™ | Webinar  hosted  by  Forensic  Magazine   Ion  Torrent™  Semiconductor  Sequencing  for  Forensic  Applica9ons   Lisa Calandro Director of Product Management Life Technologies Dr. Walther Parson Institute of Legal Medicine University of Innsbruck, Austria Available to view “on-demand”: www1.gotomeeting.com/register/134635184
  • 21Life Technologies™ | HID Workflow Software Solutions
  • 22Life Technologies™ | Converge™ Software - Integrate & Manage All Your Data, Analysis & Reporting Tools §  Modular system means easier deployment of new: −  Analysis tools (i.e. Paternity/Kinship, Database & Familial Searching) −  Lab management tools −  Data management tools −  Reporting tools Long term goal of Converge™ Centralize and integrate data, analysis and reporting across all points of the forensic DNA workflow including: −  At the crime scene −  At the police agency −  At the crime lab −  In the court room Converge™
  • 23Life Technologies™ | Our Mission: Improve Efficiency. Increase Success Rates. Provide More Info. More Workflow Efficiency Higher Success Rates More Information 4N6FLOQSwab™ & NUCLEIC-CARD™ Collection Devices ü   ParaDNA® Screening System ü   Next Gen Quantifiler® Kits ü   ü   ü   GlobalFiler® Kits ü   ü   ü   Yfiler® Plus Kit ü   ü   ü   Converge™ Software ü   ü   PGM™ for Forensics ü  
  • 24Life Technologies™ | Today’s  Agenda   Most of the presentations will be posted to SlideShare: http://www.slideshare.net/ Lifetech_HID
  • 25Life Technologies™ | THANK YOU!! Ariana Wheaton Senior Market Development Manager-Americas Life Technologies Phone: 650.638.6317 Email: ariana.wheaton@lifetechnologies.com FOR FORENSIC OR PATERNITY USE ONLY. ©2013 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners. The content provided herein may relate to products that have not been officially released and is subject to change without notice.