The fascinating new world of
STEM CELL THERAPY :
a medical revolution at the corner
Future of Stem Cell Medicine
• The Indian market potential slated to be in the range of US$ 500 million.
• More than 45,000 people across the world receiving stem cell
transplant every year.
• Potential to reduce pharmaceutical R&D by 25% each year, with
savings of up to US $25 million in each drug market.
• Potential to be the next revolution in the field of medicine.
LifeCare – ReeCure Centre
of STEM CELL Therapy
• The physical misery
• Psychological trauma
Of “No Hope Disorder” is great!!
• No cure available for most of these diseases
Rationale of is stem cell
Introduction of adult stem cells into
damaged tissue in order to treat
injuries / Disease in
“No Hope Disorders”
• Is it really working?
• Why not studies are coming from premier institutes of
• Is it really possible to segregate Mesenchymal cells?
• Differentiation of cells out side body possible in India???
• If it is too good then, why not NOBEL PRIZE given ?
• Is this therapy available in India?
We are sure there are a lot of doubts plaguing
your mind about STEM CELL BANKING and
STEM CELL THERAPY.
Let us help you to ERASE these doubts.
Doubts… on SCT
1913 the great Spanish neuroscientist Santiago Ramón y Cajal
pronounced “that in adult Neurone & the nerve paths –
Everything may die, nothing may be regenerated”.
Same is our perception
for any tissue !!
Beliefs turned out to be FALSE as
several types of cells can regenerate
Which Triggered Adult Stem Cell
BONE MARROW TRANSPLANTS
have been used for treatment of
leukemia (with Good results).
History of Human Embryonic
Stem Cell Research
In 1998, James Thomson (University of
Wisconsin-Madison) isolated cells from the inner
cell mass of the blastocyst, and developed the
first human embryonic stem cell line in culture.
Prediction was made of Making cells
and replacing all the diseased cells?
& even gametes
Stem Cell History
• 1970 – BM transplant for leukaemia
• 1998 - Researchers first extract stem cells from human
• 1999 - First Successful human transplant of insulin-making cells
• 2001 - President Bush restricts federal funding for embryonic
• 2002 - Juvenile Diabetes Research Foundation International
creates $20 million fund-raising effort to support stem-
• 2003?? - California ok stem cell research
• 2004 - Harvard researchers grow stem cells from embryos
using private funding
• 2004 - Ballot measure for $3 Billion bond for stem cells
• 2006-7 – Private Banking for cord & cord blood in india.
• 2014 – Public cord banking Kolkatta – Inst. Tropical med.
What are Stem Cells
• Ability to differentiate
• Ability to regenerate
Stem Cell is a
•primitive and unspecialized cell with a
remarkable potential to renew,
Develop into any desired tissue or organ of
What is stem cells ?
Self renewal: Unlimited proliferative potential.
Differentiation: Differentiate into various cell types
Plasticity : Trans-differentiation
Regeneration potential: a means of Repair
Cells having infinite self renewing capacity and
potential for differentiation.
What are Stem Cells?
1000 IEC ICAs in
STZ Diabetic mice
Blood glucose analysis of STZ induced
diabetic mice transplanted with hUCMSCs and
Islet like clusters (ILC)
0 10 20 30
Days after transplantation
0 25 50 75 100 125 150
non diabetic control
Fasting blood glucose concentration of diabetic mice after transplantation of ILCs showed
that the hyperglycemic mice reverted back to normoglycemia. Intraperitoneal glucose
tolerance test was performed on 45th day, post-transplantation. Mice transplanted with ILCs
showed similar blood glucose profile as compared to the normal non diabetic controls.
How MSCs work In vivo
Under normal circumstances MSC contribute to tissue maintenance, contributing to
immune homeostasis by avoiding unnecessary immune cell response.
With damaged tissue,
harmonious interaction between
MSC and endothelial cells are
disrupted, allowing blood
extravasations , blood clot form,
platelet release multitude of
bioactive molecule, immune
cells are attracted where they
start immune surveillance and
MSC secrete bioactive molecules that
exert anti-apoptotic, immunomodulatory ,
angiogenic , anti-scaring , anti-fibrotic
and chemoattractant effects
Source of Adult Stem cells
• Allogeneic or
Fetal tissue is the best current tissue source for
human stem cells,
However ethical issues are a major concern.
(ICMR – Autologous)
The embryos of
and cord blood of a
new born baby
Adult Tissues Amniotic Fluid
MENSTRUAL BLOOD Dental Pulp
Sources of Stem Cells
Cord blood -
Rich Source of
Cord Blood Stem Cells
• Can be used only
to cure chronic
disorders such as
Umbilical Cord Blood Stem Cell
• Umbilical cord blood
stem cell transplants are
less prone to
rejection than either
bone marrow or
peripheral blood stem
cells. This is probably
because the cells have
not yet developed the
features that can be
recognized and attacked
by the recipient's
Placental Blood as a Source of Hematopoietic
Stem Cells for Transplantation into
Joanne Kurtzberg, M.D.et al
PLASTICITY: The ability of one tissue to produce cells of other developmentally
Traditionally, stem cells were believed to be Lineage Restricted and
Bone marrow stem cells:
The gold standard
AUTOLOGUS – STEM CELLS
• While most blood stem
cells reside in the bone
marrow, a small number
are present in the
blood stem cells, or
PBSCs, can be used
just like bone marrow
stem cells to treat
cancers and various
Allogeneic – Stem Cells
• Sources of stem cells
from another donor
(familial- allogeneic) or
allogeneic). The stem
cells in this situation
are extracted from
either the donor's body
or cord blood
Xenogeny - Stem Cells
• In this stem cells from
different species are
transplanted, e.g. porcine
fetal ventral mesencephalic
(FVM) xenotransplants for
This has no major ethical
concerns and a large amount
of tissue is available,
however life long
immunosupression and risk
of rejection are the major
Prediction for Future
Cells from these Animal will
be used in human beings
Without risk of rejection
• The cells reach
the target cell or
• Cells transform
into target cell or
cell or tissue for
THE SUCCESS OF STEM CELL
THERAPY DEPENDS ON LAB.
• Mono Nuclear cells ( MNC )
• Hematopoietic Stem Cells [HSC] family:
CD34+; CD45; CD133
• Mesenchymel count in M.N.C is just 0.001%
• Mesenchymel stem cell [MSC]: CD73; CD90;
CD106 Etc ..........
Classification Of Stem Cells
Differentiation with different cluster
coding/desire cells type ( as per disorder)
through culture.. (Mesenchymal types are -
CD 73, CD 90, CD 105 etc . .. . .
• Multiplication up label of required cells
quantity.( near to 1.5 Cr.).
• Composition of all cells like Mnc, Hsc & Msc.
Technology Behind Success
high rate of cell
No risk of
rejection – USED
No risk of
Lower cost of
Why MSCs ?
Not Effective/ Need More Trails
Acute Leukaemia • Inherited Platelet Abnormalities
• Chronic Leukaemia • Other Inherited Disorder
• Myelodysplastic Syndromes • Inherited Metabolic Disorders
• Marrow Failure • Histiocytic Disorders
• Inherited Erythrocyte
• Myeloproliferative Disorders
• Inherited Immune System
• Plasma Cell Disorders
• Phagocyte Disorders • Other Malignancies
Drug Testing - Quicker
• Stem cells could allow scientists to test
new drugs using human cell line which
could speed up new drug development.
• Only drugs that were safe and had
beneficial effects in cell line testing would
graduate to whole animal or human
• It would allow quicker and safer
development of new drugs.
Create a better tomorrow
Spread the message
Please create Public Awareness
My cord blood treated
Bhai’s thalassemia major Cured of insulin dep. DM
Only company in India with 9 type of stem cell
banking and treatment through Reecure
50 centres spread across India
Mr. Ajay Saxena
Website : www.reelabs.com
Email : email@example.com
Q.& A.session Dr. Sharda Jain
Mr. Ajay Saxena
A HOPE TO NO