Ambion Cells-to-Ct
 

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Fact sheet for Ambion® Cells-to-Ct™ Kits new green features - compared to traditional RNA Extraction methods that contain harmful solvents such as Mercaptoethanol—which can be fatal.

Fact sheet for Ambion® Cells-to-Ct™ Kits new green features - compared to traditional RNA Extraction methods that contain harmful solvents such as Mercaptoethanol—which can be fatal.

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Ambion Cells-to-Ct Document Transcript

  • 1. Green featuresFact SheetAmbion® Cells-to-Ct™ KitsComparison to Traditional RNA Extraction Methods Introduction • Mercaptoethanol—may be fatal Life Technologies is committed when absorbed through the skin to designing products with the • Guanidine thiocyanate—causes environment in mind—it’s one more irritation and is harmful if step toward a smaller footprint. This swallowed or inhaled fact sheet provides the rationale • Guanidine hydrochloride—causes Green Benefits behind the environmental claims that irritation and is harmful if • Less use of hazardous materials use of this product results in reduced swallowed or inhaled • Less hazardous waste disposal exposure to hazardous material and generates less waste than comparable The use of Cells-to-Ct™ systems • Less use of plastic consumables products. The use of Ambion® Cells- eliminates the need to use any of the • Less plastic waste disposal to-Ct™ kits eliminates the need to use hazardous solvents mentioned above. hazardous solvents and requires far less plastic consumables from sample Please review the MSDS for the preparation to final analysis. Cells-to-Ct™ kits at http://www3. appliedbiosystems.com/sup/msds/ Product Description search.htm Ambion® Cells-to-Ct™ kits include reagents and enzyme mixtures Less Waste for reverse transcription (RT) and Traditional methodologies for RNA real-time PCR directly from cultured extraction require multiple steps cell lysates, without the need for a for RNA extraction and clean-up, separate RNA isolation step. requiring the use of multiple disposable tubes, vials, pipettes, and Green Features pipette tips. Cells-to-Ct™ kits require Less Hazardous fewer plastic consumables than Traditional RNA extraction protocols traditional technologies (Figure 1). A require clean-up with the use of comparison of Cells-to-Ct™ kits with hazardous reagents such as: traditional technology showed that 139 g of plastic waste (tubes, pipettes, • Ethanol—highly flammable and pipette tips) was generated with causes systemic toxicity traditional RNA extraction, compared to 7 g for Cells-to-Ct™ kits (Table 1).
  • 2. A BFigure 1. Comparison of Plastic Waste Generated Using Traditional RNA Extraction Methods (A) vs. Cells-to-Ct™ Kits (B). Table 1. Comparison of the Amount of Waste Generated Using Traditional RNA Extraction Methods vs. Cells-to-Ct™ Kits. Traditional RNA extraction methods Cells-to-Ct™ kits Step in Plastic Piece Total Step in Plastic Piece Total procedure description # Used weight (g) mass (g) procedure description # Used weight (g) mass (g) Add 100% 50 mL tip 1 20.75 20.75 Tube for lysis 1.5 mL 1 1.00 1.00 ethanol to RPE mix tube Add B-ME to 1 mL tip 1 0.85 0.85 Add lysis mix 1 mL tip 1 0.85 0.85 RLT to tube Tube for 50 mL 1 12.54 12.54 Add DNase to 20 µL tip 1 0.18 0.18 hazardous tube tube waste Add 350 1 mL tip 10 0.85 8.50 Add lysis 200 µL tip 10 0.28 2.80 µL RLT to solution to samples samples, mix Add 70% 1 mL tip 10 0.85 8.50 Add stop 20 µL tip 10 0.18 1.80 ethanol to solution to samples samples, mix Add 500 1 mL tip 10 0.85 8.50 Total used 23 6.63 µL RPE to samples Add another 1 mL tip 10 0.85 8.50 500 µL RPE Tubes for 1.5 mL 10 1.00 10.00 samples tube Add water to 200 µL tip 10 0.28 2.80 elute Add water to 200 µL tip 10 0.28 2.80 elute again gDNA column, 10 1.65 16.50 eliminator tube columns RNeasy column, 10 2.93 29.30 (Qiagen) spin tube columns 2 mL Tube 10 1.00 10.00 collection tubes Total used 103 139.54 Waste 95.2% reductionFor Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.© 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.Printed in the USA. CO21121-2 0711Headquarters5791 Van Allen Way | Carlsbad, CA 92008 USA | Phone +1.760.603.7200 | Toll Free in the USA 800.955.6288www.lifetechnologies.com