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Using Magnetic Beads in ChIP

by on Feb 09, 2012

  • 589 views

Loni Pickle, Life Technologies R&D Scientist, highlights some of the benefits of using magnetic beads in chromatin immunoprecipitation (ChIP) sample processing. ...

Loni Pickle, Life Technologies R&D Scientist, highlights some of the benefits of using magnetic beads in chromatin immunoprecipitation (ChIP) sample processing.

Tissue Processing Using Magnetic Beads -- Shearing Optimization

[audio transcript]

This is Loni Pickle, Scientist at Life Technologies; the next area I'd like to focus on is using magnetic beads in both the immunoprecipitation and DNA purification step.

I used to use sepharose beads in my ChIP protocol, but a few years ago I switched to magnetic beads and here's why:

Instead of pipetting eight 1.5 ml tubes at a time, here we switch to a system where you can magnetize 8 tubes at once, and also wash 8 tubes at a time using a multi-channel pipette, making it extremely easy, faster and more efficient to use.

Magnetic beads - particularly Dynabeads - are lower background binding, which is really important especially when heading into the sequencing field where background can cost you time, money, and your precious sample.

More and more researchers are switching to magnetic beads for the immunoprecipitation part of their ChIP, but another great place to use them is during the DNA magnetic purification.

So here's why I switched to magnetic beads for the DNA purification part of the ChIP protocol: Just like with immunoprecipitation, DNA magnetic beads will pellet conveniently on the side of your tube; again making washing easier, faster, and more efficient.

Lastly, but certainly not the least, was the environmental factor: Personally I didn't want to use reagents that were toxic if I didn't need to.

For more information on Dynabeads magnetic separation technology, visit http://www.invitrogen.com/Dynabeads

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